BRENDA - Enzyme Database show
show all sequences of 1.1.1.79

Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate

Yoshikawa, S.; Arai, R.; Kinoshita, Y.; Uchikubo-Kamo, T.; Wakamatsu, T.; Akasaka, R.; Masui, R.; Terada, T.; Kuramitsu, S.; Shirouzu, M.; Yokoyama, S.; Acta Crystallogr. Sect. D 63, 357-365 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL and Escherichia coli B834(DE3)pRARE
Pyrococcus horikoshii OT3
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method in the presence of NAD, crystal structure analysis reveals tightly bound NADP(H) at the enzyme originating from Escherichia coli expression, which is not replaceable by NAD
Pyrococcus horikoshii OT3
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
NADH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
0.015
-
NADH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
0.021
-
NADPH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
0.025
-
NADPH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000, SDS-PAGE, native mass by analytical ultracentrifugation
Pyrococcus horikoshii OT3
76500
-
analytical ultracentrifugation
Pyrococcus horikoshii OT3
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus horikoshii OT3
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein from Escherichia coli
Pyrococcus horikoshii OT3
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADH + H+
-
684141
Pyrococcus horikoshii OT3
glycolate + NAD+
-
-
-
?
glyoxylate + NADPH + H+
-
684141
Pyrococcus horikoshii OT3
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NADH + H+
-
684141
Pyrococcus horikoshii OT3
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
684141
Pyrococcus horikoshii OT3
D-glycerate + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
x * 38000, SDS-PAGE, native mass by analytical ultracentrifugation
Pyrococcus horikoshii OT3
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8
-
NADPH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
2.4
-
NADPH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
4.1
-
NADH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
11
-
NADH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
enzyme has a higher affinity for NADPH than for NADH when incubated without substrate
Pyrococcus horikoshii OT3
NADPH
enzyme has a higher affinity for NADPH than for NADH when incubated without substrate
Pyrococcus horikoshii OT3
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL and Escherichia coli B834(DE3)pRARE
Pyrococcus horikoshii OT3
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
enzyme has a higher affinity for NADPH than for NADH when incubated without substrate
Pyrococcus horikoshii OT3
NADPH
enzyme has a higher affinity for NADPH than for NADH when incubated without substrate
Pyrococcus horikoshii OT3
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method in the presence of NAD, crystal structure analysis reveals tightly bound NADP(H) at the enzyme originating from Escherichia coli expression, which is not replaceable by NAD
Pyrococcus horikoshii OT3
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
NADH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
0.015
-
NADH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
0.021
-
NADPH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
0.025
-
NADPH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000, SDS-PAGE, native mass by analytical ultracentrifugation
Pyrococcus horikoshii OT3
76500
-
analytical ultracentrifugation
Pyrococcus horikoshii OT3
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein from Escherichia coli
Pyrococcus horikoshii OT3
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADH + H+
-
684141
Pyrococcus horikoshii OT3
glycolate + NAD+
-
-
-
?
glyoxylate + NADPH + H+
-
684141
Pyrococcus horikoshii OT3
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NADH + H+
-
684141
Pyrococcus horikoshii OT3
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
684141
Pyrococcus horikoshii OT3
D-glycerate + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
x * 38000, SDS-PAGE, native mass by analytical ultracentrifugation
Pyrococcus horikoshii OT3
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8
-
NADPH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
2.4
-
NADPH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
4.1
-
NADH
with hydroxypyruvate as substrate, pH 7.5
Pyrococcus horikoshii OT3
11
-
NADH
with glyoxylate as substrate, pH 7.5
Pyrococcus horikoshii OT3
Other publictions for EC 1.1.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
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-
3
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1
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-
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1
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1
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-
-
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-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
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-
-
3
-
-
2
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-
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-
-
4
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1
4
-
-
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-
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-
2
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-
2
-
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-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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1
-
-
2
-
-
-
-
-
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685774
Ogino
Characterization of recombinan ...
Thermus thermophilus, Thermus thermophilus HB27
Biotechnol. Prog.
24
321-325
2008
-
-
2
-
-
-
3
8
-
-
1
2
10
2
-
-
2
-
-
-
-
-
5
1
2
-
4
8
1
1
-
4
-
-
-
-
-
2
4
-
-
-
-
3
-
8
-
-
1
2
10
-
-
2
-
-
-
-
5
1
2
-
4
8
1
1
-
-
-
-
-
-
-
-
688090
Simpson
Identification and characteriz ...
Arabidopsis thaliana
J. Exp. Bot.
59
2545-2554
2008
-
-
1
-
2
-
1
4
2
-
4
4
-
7
-
-
1
-
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
2
-
-
-
2
4
-
2
-
-
1
-
4
2
-
4
4
-
-
-
1
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
-
-
-
-
-
688091
Allan
Gamma-hydroxybutyrate accumula ...
Arabidopsis thaliana, Nicotiana tabacum
J. Exp. Bot.
59
2555-2564
2008
-
-
-
-
-
-
2
-
2
-
-
5
-
5
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
2
-
-
5
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
-
-
1
1
-
-
-
4
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
4
-
-
-
2
-
-
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-
-
1
2
1
-
-
-
-
-
4
-
-
2
-
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-
1
-
-
-
-
4
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
685902
Hoover
Kinetic mechanism of a recombi ...
Arabidopsis thaliana
Can. J. Bot.
85
896-902
2007
-
-
1
-
-
-
3
2
-
-
-
-
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
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-
1
8
-
-
-
-
1
1
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3
8
2
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1
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2
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690207
Rintala
The ORF YNL274c (GOR1) codes f ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Yeast
24
129-136
2007
-
-
1
-
1
-
-
-
-
-
-
2
-
21
-
-
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-
-
-
3
-
6
-
1
-
-
-
1
-
-
2
-
-
-
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-
1
2
-
1
-
-
-
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2
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-
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-
-
-
3
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
669886
Booth
Structural basis of substrate ...
Homo sapiens
J. Mol. Biol.
360
178-189
2006
-
-
1
1
4
-
1
-
-
-
-
3
-
2
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1
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2
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5
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1
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2
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1
2
1
4
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1
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3
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1
-
2
-
-
5
-
-
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1
-
-
-
-
-
-
-
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667814
Mdluli
A preliminary account of the p ...
Homo sapiens
Biochim. Biophys. Acta
1753
209-216
2005
-
-
1
-
-
-
-
7
1
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2
-
1
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-
1
-
8
-
1
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4
1
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2
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1
2
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7
1
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2
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1
-
8
-
1
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4
1
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-
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-
-
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-
669351
Genolet
Promoter rearrangements cause ...
Homo sapiens, Mus musculus
J. Biol. Chem.
280
24143-24152
2005
-
-
1
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2
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5
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2
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2
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1
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2
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2
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287318
Kleczkowski
-
Kinetics and regulation of the ...
Spinacia oleracea
Z. Naturforsch. C
50
21-28
1995
1
-
-
-
-
-
15
4
1
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1
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1
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-
-
1
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1
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2
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1
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2
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15
-
4
1
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1
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1
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1
-
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-
-
-
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-
-
-
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-
-
-
-
287319
Husic
NADH:hydroxypyruvate reductase ...
Chlamydomonas reinhardtii
Arch. Biochem. Biophys.
252
396-408
1987
-
-
-
-
-
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-
1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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-
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-
287320
Kleczkowski
Purification and characterizat ...
Glycine max, Spinacia oleracea, Triticum aestivum
Biochem. J.
239
653-659
1986
-
-
-
-
-
-
-
4
-
-
2
3
-
3
-
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1
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-
1
3
-
3
1
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1
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3
-
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-
3
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4
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2
3
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1
-
1
3
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287321
Yokota
Purification and some properti ...
Euglena gracilis
Biochem. J.
227
211-216
1985
-
-
-
-
-
-
4
2
3
-
1
1
-
3
-
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1
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1
1
-
4
-
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1
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2
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2
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4
-
2
3
-
1
1
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-
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1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
287322
Mulligan
Effects of glyoxylate on photo ...
Spinacia oleracea
Plant Physiol.
72
415-419
1983
-
-
-
-
-
-
-
1
1
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-
1
-
1
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-
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1
-
1
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1
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1
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-
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1
1
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1
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-
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1
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1
-
-
-
-
-
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-
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-
-
-
286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
1
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-
1
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1
-
-
1
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-
-
-
1
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
287324
Fukuda
Purification and properties of ...
Neurospora crassa
Agric. Biol. Chem.
45
1153-1158
1981
-
-
-
-
-
-
2
4
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
4
-
-
1
1
-
-
-
1
-
-
1
1
4
-
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-
1
-
2
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-
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-
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287325
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1
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1
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1
-
-
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1
1
3
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1
-
2
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1
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1
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4
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4
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1
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1
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1
1
3
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1
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2
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Sagisaka
Occurrence of nicotinamide ade ...
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1
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1
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1
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1
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1
1
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1
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-
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1
1
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2
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2
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1
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1
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-
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1
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1
1
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1
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1
1
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287327
Yokota
Occurrence and properties of t ...
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2
1
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1
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1
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1
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1
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2
3
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1
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2
1
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1
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1
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Tochikura
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1
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1
1
5
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1
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1
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2
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2
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10
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2
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1
1
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1
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1
1
5
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1
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1
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Zeltich
Effect of glycidate, an inhibi ...
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
1
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1
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