BRENDA - Enzyme Database show
show all sequences of 1.1.1.79

Glyoxylate reductase isoform 1 is localized in the cytosol and not peroxisomes in plant cells

Ching, S.L.; Gidda, S.K.; Rochon, A.; van Cauwenberghe, O.R.; Shelp, B.J.; Mullen, R.T.; J. Integr. Plant Biol. 54, 152-168 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
GLYR1 is exclusively located in the cytosol
Arabidopsis thaliana
5829
-
additional information
GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycolate + NAD+
Arabidopsis thaliana
-
glyoxylate + NADH + H+
-
-
r
glycolate + NADP+
Arabidopsis thaliana
-
glyoxylate + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LSV0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycolate + NAD+
-
722899
Arabidopsis thaliana
glyoxylate + NADH + H+
-
-
-
r
glycolate + NADP+
-
722899
Arabidopsis thaliana
glyoxylate + NADPH + H+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
GLYR1 is exclusively located in the cytosol
Arabidopsis thaliana
5829
-
additional information
GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycolate + NAD+
Arabidopsis thaliana
-
glyoxylate + NADH + H+
-
-
r
glycolate + NADP+
Arabidopsis thaliana
-
glyoxylate + NADPH + H+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycolate + NAD+
-
722899
Arabidopsis thaliana
glyoxylate + NADH + H+
-
-
-
r
glycolate + NADP+
-
722899
Arabidopsis thaliana
glyoxylate + NADPH + H+
-
-
-
r
General Information
General Information
Commentary
Organism
additional information
due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
physiological function
GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
additional information
due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
physiological function
GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively
Arabidopsis thaliana
Other publictions for EC 1.1.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
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1
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1
1
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722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
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1
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2
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696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
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685774
Ogino
Characterization of recombinan ...
Thermus thermophilus, Thermus thermophilus HB27
Biotechnol. Prog.
24
321-325
2008
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2
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3
8
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1
2
10
2
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1
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8
1
1
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10
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1
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8
1
1
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688090
Simpson
Identification and characteriz ...
Arabidopsis thaliana
J. Exp. Bot.
59
2545-2554
2008
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1
-
2
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1
4
2
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7
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1
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1
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15
1
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1
1
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2
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2
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1
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4
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1
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4
15
1
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4
1
1
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2
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688091
Allan
Gamma-hydroxybutyrate accumula ...
Arabidopsis thaliana, Nicotiana tabacum
J. Exp. Bot.
59
2555-2564
2008
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5
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7
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7
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684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
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1
1
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4
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1
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4
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685902
Hoover
Kinetic mechanism of a recombi ...
Arabidopsis thaliana
Can. J. Bot.
85
896-902
2007
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1
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3
2
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690207
Rintala
The ORF YNL274c (GOR1) codes f ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Yeast
24
129-136
2007
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1
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21
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6
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1
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1
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669886
Booth
Structural basis of substrate ...
Homo sapiens
J. Mol. Biol.
360
178-189
2006
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1
1
4
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1
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2
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2
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5
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1
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667814
Mdluli
A preliminary account of the p ...
Homo sapiens
Biochim. Biophys. Acta
1753
209-216
2005
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1
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7
1
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1
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669351
Genolet
Promoter rearrangements cause ...
Homo sapiens, Mus musculus
J. Biol. Chem.
280
24143-24152
2005
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287318
Kleczkowski
-
Kinetics and regulation of the ...
Spinacia oleracea
Z. Naturforsch. C
50
21-28
1995
1
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15
4
1
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287319
Husic
NADH:hydroxypyruvate reductase ...
Chlamydomonas reinhardtii
Arch. Biochem. Biophys.
252
396-408
1987
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1
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287320
Kleczkowski
Purification and characterizat ...
Glycine max, Spinacia oleracea, Triticum aestivum
Biochem. J.
239
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1986
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287321
Yokota
Purification and some properti ...
Euglena gracilis
Biochem. J.
227
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1985
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4
2
3
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287322
Mulligan
Effects of glyoxylate on photo ...
Spinacia oleracea
Plant Physiol.
72
415-419
1983
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286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
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287324
Fukuda
Purification and properties of ...
Neurospora crassa
Agric. Biol. Chem.
45
1153-1158
1981
-
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2
4
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1
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1
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1
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1
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287325
Fukuda
Purification and enzymatic pro ...
Saccharomyces cerevisiae
J. Biochem.
87
841-846
1980
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4
4
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1
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1
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1
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3
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1
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4
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1
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1
3
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1
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2
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287326
Sagisaka
Occurrence of nicotinamide ade ...
Populus gelrica
Plant Physiol.
65
377-381
1980
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1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287327
Yokota
Occurrence and properties of t ...
Euglena gracilis
Biochem. J.
184
189-192
1979
-
-
-
-
-
-
-
2
3
-
-
1
-
5
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
3
-
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287328
Tochikura
Purification and properties of ...
Saccharomyces cerevisiae
J. Biochem.
86
105-110
1979
-
-
-
-
-
-
10
2
-
-
1
1
-
1
-
-
1
-
-
-
1
1
5
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
-
1
1
-
-
-
1
-
-
1
1
5
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
287329
Zeltich
Effect of glycidate, an inhibi ...
Nicotiana tabacum
Plant Physiol.
61
236-241
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-