BRENDA - Enzyme Database show
show all sequences of 1.1.1.79

Characterization of recombinant glyoxylate reductase from thermophile Thermus thermophilus HB27

Ogino, H.; Nakayama, H.; China, H.; Kawata, T.; Doukyu, N.; Yasuda, M.; Biotechnol. Prog. 24, 321-325 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Top10 as His-tag fusion protein
Thermus thermophilus HB27
expression of the Myc-His9-tagged enzyme in Escherichia coli
Thermus thermophilus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no activity at more than 500 mM NaCl, 50% activity in the presence of 900 mM sodium phosphate
Thermus thermophilus HB27
NaCl
complete inhibition at 0.5 M
Thermus thermophilus
Sodium phosphate
50% inhibition at 900 mM
Thermus thermophilus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
NADPH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
0.04
-
NADPH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
0.076
-
NADH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
0.076
-
NADH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
0.5
-
glyoxylate
pH 6.7, 45°C, cofactor NADPH, purified recombinant enzyme
Thermus thermophilus
0.5
-
glyoxylate
with as NADPH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
1.2
-
glyoxylate
pH 6.7, 45°C, cofactor NADH, purified recombinant enzyme
Thermus thermophilus
1.2
-
glyoxylate
with as NADH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, SDS-PAGE, native mass by gel filtration (native mass not given in the reference)
Thermus thermophilus HB27
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NAD(P)H
Thermus thermophilus
-
glycolate + NAD(P)+
-
-
?
glyoxylate + NADPH + H+
Thermus thermophilus HB27
-
glycolate + NADP+
-
-
ir
Organic Solvent Stability
Organic Solvent
Commentary
Organism
1,5-pentanediol
25%, half life reduced by approximately 70%
Thermus thermophilus HB27
diethylene glycol
half-life in the presence of 25% diethylene glycol significantly longer than that in the absence of an organic solvent
Thermus thermophilus HB27
dimethyl sulfoxide
half-life in the presence of 25% dimethyl sulfoxide significantly longer than that in the absence of an organic solvent
Thermus thermophilus HB27
Ethanol
25%, half life reduced by approximately 70%
Thermus thermophilus HB27
Glycerol
25%, unstable
Thermus thermophilus HB27
n-decane
25%, unstable
Thermus thermophilus HB27
n-hexane
25%, unstable
Thermus thermophilus HB27
n-octane
25%, unstable
Thermus thermophilus HB27
p-xylene
25%, unstable
Thermus thermophilus HB27
toluene
25%, unstable
Thermus thermophilus HB27
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermus thermophilus
-
strain HB27
-
Thermus thermophilus HB27
-
NBRC code 101085
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His9-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity
Thermus thermophilus
recombinant protein from Escherichia coli using His-tag
Thermus thermophilus HB27
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NAD(P)H
-
685774
Thermus thermophilus
glycolate + NAD(P)+
-
-
-
?
glyoxylate + NADH + H+
-
685774
Thermus thermophilus HB27
glycolate + NAD+
-
-
-
ir
glyoxylate + NADPH + H+
-
685774
Thermus thermophilus HB27
glycolate + NADP+
-
-
-
ir
additional information
phenylpyruvate, pyruvate, methylglyoxal, malate, and oxaloacetate not suitable as substrate
685774
Thermus thermophilus HB27
?
-
-
-
-
additional information
substrate specificity, several 2-oxo compounds including phenylpyruvate, pyruvate, methylglyoxal, and oxaloacetate act as inert electron acceptors, as do glycolate and malate, overview
685774
Thermus thermophilus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 36000, SDS-PAGE, native mass by gel filtration (native mass not given in the reference)
Thermus thermophilus HB27
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
recombinant protein
Thermus thermophilus HB27
45
-
recombinant enzyme
Thermus thermophilus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
10 min, recombinant enzyme, stable
Thermus thermophilus
70
-
stable up to 70°C
Thermus thermophilus HB27
80
-
complete loss of activity within 10 min when incubates above 80°C
Thermus thermophilus HB27
82
-
melting point
Thermus thermophilus HB27
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
22000
-
NADH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
30000
-
NADPH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
65000
-
glyoxylate
pH 6.7, 45°C, cofactor NADPH, purified recombinant enzyme
Thermus thermophilus
76000
-
glyoxylate
pH 6.7, 45°C, cofactor NADH, purified recombinant enzyme
Thermus thermophilus
220000
-
NADH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
300000
-
NADPH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
650000
-
glyoxylate
with as NADPH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
760000
-
glyoxylate
with as NADH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
recombinant enzyme
Thermus thermophilus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.7
-
recombinant protein
Thermus thermophilus HB27
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
less active than NADPH
Thermus thermophilus
NADH
yields higher Km but similar turnover than NADPH
Thermus thermophilus HB27
NADPH
is the preferred cofactor
Thermus thermophilus
NADPH
preferred cofactor, yields lower Km but similar turnover than NADH
Thermus thermophilus HB27
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Top10 as His-tag fusion protein
Thermus thermophilus HB27
expression of the Myc-His9-tagged enzyme in Escherichia coli
Thermus thermophilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
less active than NADPH
Thermus thermophilus
NADH
yields higher Km but similar turnover than NADPH
Thermus thermophilus HB27
NADPH
is the preferred cofactor
Thermus thermophilus
NADPH
preferred cofactor, yields lower Km but similar turnover than NADH
Thermus thermophilus HB27
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no activity at more than 500 mM NaCl, 50% activity in the presence of 900 mM sodium phosphate
Thermus thermophilus HB27
NaCl
complete inhibition at 0.5 M
Thermus thermophilus
Sodium phosphate
50% inhibition at 900 mM
Thermus thermophilus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
NADPH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
0.04
-
NADPH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
0.076
-
NADH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
0.076
-
NADH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
0.5
-
glyoxylate
pH 6.7, 45°C, cofactor NADPH, purified recombinant enzyme
Thermus thermophilus
0.5
-
glyoxylate
with as NADPH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
1.2
-
glyoxylate
pH 6.7, 45°C, cofactor NADH, purified recombinant enzyme
Thermus thermophilus
1.2
-
glyoxylate
with as NADH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, SDS-PAGE, native mass by gel filtration (native mass not given in the reference)
Thermus thermophilus HB27
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NAD(P)H
Thermus thermophilus
-
glycolate + NAD(P)+
-
-
?
glyoxylate + NADPH + H+
Thermus thermophilus HB27
-
glycolate + NADP+
-
-
ir
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
1,5-pentanediol
25%, half life reduced by approximately 70%
Thermus thermophilus HB27
diethylene glycol
half-life in the presence of 25% diethylene glycol significantly longer than that in the absence of an organic solvent
Thermus thermophilus HB27
dimethyl sulfoxide
half-life in the presence of 25% dimethyl sulfoxide significantly longer than that in the absence of an organic solvent
Thermus thermophilus HB27
Ethanol
25%, half life reduced by approximately 70%
Thermus thermophilus HB27
Glycerol
25%, unstable
Thermus thermophilus HB27
n-decane
25%, unstable
Thermus thermophilus HB27
n-hexane
25%, unstable
Thermus thermophilus HB27
n-octane
25%, unstable
Thermus thermophilus HB27
p-xylene
25%, unstable
Thermus thermophilus HB27
toluene
25%, unstable
Thermus thermophilus HB27
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His9-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity
Thermus thermophilus
recombinant protein from Escherichia coli using His-tag
Thermus thermophilus HB27
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NAD(P)H
-
685774
Thermus thermophilus
glycolate + NAD(P)+
-
-
-
?
glyoxylate + NADH + H+
-
685774
Thermus thermophilus HB27
glycolate + NAD+
-
-
-
ir
glyoxylate + NADPH + H+
-
685774
Thermus thermophilus HB27
glycolate + NADP+
-
-
-
ir
additional information
phenylpyruvate, pyruvate, methylglyoxal, malate, and oxaloacetate not suitable as substrate
685774
Thermus thermophilus HB27
?
-
-
-
-
additional information
substrate specificity, several 2-oxo compounds including phenylpyruvate, pyruvate, methylglyoxal, and oxaloacetate act as inert electron acceptors, as do glycolate and malate, overview
685774
Thermus thermophilus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 36000, SDS-PAGE, native mass by gel filtration (native mass not given in the reference)
Thermus thermophilus HB27
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
recombinant enzyme
Thermus thermophilus
45
-
recombinant protein
Thermus thermophilus HB27
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
10 min, recombinant enzyme, stable
Thermus thermophilus
70
-
stable up to 70°C
Thermus thermophilus HB27
80
-
complete loss of activity within 10 min when incubates above 80°C
Thermus thermophilus HB27
82
-
melting point
Thermus thermophilus HB27
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
22000
-
NADH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
30000
-
NADPH
pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme
Thermus thermophilus
65000
-
glyoxylate
pH 6.7, 45°C, cofactor NADPH, purified recombinant enzyme
Thermus thermophilus
76000
-
glyoxylate
pH 6.7, 45°C, cofactor NADH, purified recombinant enzyme
Thermus thermophilus
220000
-
NADH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
300000
-
NADPH
with glyoxylate as substrate, pH 6.7, 45°C
Thermus thermophilus HB27
650000
-
glyoxylate
with as NADPH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
760000
-
glyoxylate
with as NADH as cofactor, pH 6.7, 45°C
Thermus thermophilus HB27
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
recombinant enzyme
Thermus thermophilus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.7
-
recombinant protein
Thermus thermophilus HB27
Other publictions for EC 1.1.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685774
Ogino
Characterization of recombinan ...
Thermus thermophilus, Thermus thermophilus HB27
Biotechnol. Prog.
24
321-325
2008
-
-
2
-
-
-
3
8
-
-
1
2
10
2
-
-
2
-
-
-
-
-
5
1
2
-
4
8
1
1
-
4
-
-
-
-
-
2
4
-
-
-
-
3
-
8
-
-
1
2
10
-
-
2
-
-
-
-
5
1
2
-
4
8
1
1
-
-
-
-
-
-
-
-
688090
Simpson
Identification and characteriz ...
Arabidopsis thaliana
J. Exp. Bot.
59
2545-2554
2008
-
-
1
-
2
-
1
4
2
-
4
4
-
7
-
-
1
-
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
2
-
-
-
2
4
-
2
-
-
1
-
4
2
-
4
4
-
-
-
1
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
-
-
-
-
-
688091
Allan
Gamma-hydroxybutyrate accumula ...
Arabidopsis thaliana, Nicotiana tabacum
J. Exp. Bot.
59
2555-2564
2008
-
-
-
-
-
-
2
-
2
-
-
5
-
5
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
2
-
-
5
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
-
-
1
1
-
-
-
4
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
4
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
4
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
685902
Hoover
Kinetic mechanism of a recombi ...
Arabidopsis thaliana
Can. J. Bot.
85
896-902
2007
-
-
1
-
-
-
3
2
-
-
-
-
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
8
-
-
-
-
1
1
-
-
-
-
3
8
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690207
Rintala
The ORF YNL274c (GOR1) codes f ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Yeast
24
129-136
2007
-
-
1
-
1
-
-
-
-
-
-
2
-
21
-
-
-
-
-
-
3
-
6
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
669886
Booth
Structural basis of substrate ...
Homo sapiens
J. Mol. Biol.
360
178-189
2006
-
-
1
1
4
-
1
-
-
-
-
3
-
2
-
-
1
-
-
2
-
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
1
4
-
-
1
-
-
-
-
-
3
-
-
-
1
-
2
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
667814
Mdluli
A preliminary account of the p ...
Homo sapiens
Biochim. Biophys. Acta
1753
209-216
2005
-
-
1
-
-
-
-
7
1
-
-
2
-
1
-
-
-
-
-
-
1
-
8
-
1
-
-
4
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
7
1
-
-
2
-
-
-
-
-
-
1
-
8
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
669351
Genolet
Promoter rearrangements cause ...
Homo sapiens, Mus musculus
J. Biol. Chem.
280
24143-24152
2005
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287318
Kleczkowski
-
Kinetics and regulation of the ...
Spinacia oleracea
Z. Naturforsch. C
50
21-28
1995
1
-
-
-
-
-
15
4
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
15
-
4
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287319
Husic
NADH:hydroxypyruvate reductase ...
Chlamydomonas reinhardtii
Arch. Biochem. Biophys.
252
396-408
1987
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287320
Kleczkowski
Purification and characterizat ...
Glycine max, Spinacia oleracea, Triticum aestivum
Biochem. J.
239
653-659
1986
-
-
-
-
-
-
-
4
-
-
2
3
-
3
-
-
1
-
-
1
3
-
3
1
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
4
-
-
2
3
-
-
-
1
-
1
3
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287321
Yokota
Purification and some properti ...
Euglena gracilis
Biochem. J.
227
211-216
1985
-
-
-
-
-
-
4
2
3
-
1
1
-
3
-
-
1
-
-
1
1
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
2
3
-
1
1
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287322
Mulligan
Effects of glyoxylate on photo ...
Spinacia oleracea
Plant Physiol.
72
415-419
1983
-
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287324
Fukuda
Purification and properties of ...
Neurospora crassa
Agric. Biol. Chem.
45
1153-1158
1981
-
-
-
-
-
-
2
4
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
4
-
-
1
1
-
-
-
1
-
-
1
1
4
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287325
Fukuda
Purification and enzymatic pro ...
Saccharomyces cerevisiae
J. Biochem.
87
841-846
1980
-
-
-
-
-
-
4
4
-
-
-
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
4
-
-
-
1
-
-
-
1
-
-
1
1
3
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287326
Sagisaka
Occurrence of nicotinamide ade ...
Populus gelrica
Plant Physiol.
65
377-381
1980
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287327
Yokota
Occurrence and properties of t ...
Euglena gracilis
Biochem. J.
184
189-192
1979
-
-
-
-
-
-
-
2
3
-
-
1
-
5
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
3
-
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287328
Tochikura
Purification and properties of ...
Saccharomyces cerevisiae
J. Biochem.
86
105-110
1979
-
-
-
-
-
-
10
2
-
-
1
1
-
1
-
-
1
-
-
-
1
1
5
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
-
1
1
-
-
-
1
-
-
1
1
5
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
287329
Zeltich
Effect of glycidate, an inhibi ...
Nicotiana tabacum
Plant Physiol.
61
236-241
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-