1.1.1.79: glyoxylate reductase (NADP+)

This is an abbreviated version, for detailed information about glyoxylate reductase (NADP+), go to the full flat file.

Reaction

glycolate
+
NADP+
=
glyoxylate
+
NADPH
+
H+

Synonyms

AtGR1, AtGR2, D-2-hydroxy-acid dehydrogenase, glyoxylate reductase, glyoxylate reductase 1, glyoxylate reductase 2, glyoxylate reductase isoform 1, glyoxylate reductase/hydroxypyruvate reductase, GLYR1, GLYR2, GOR1, GR/HPR, GR1, GR2, GRHPR, GRHRP, More, TthGR1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.79 glyoxylate reductase (NADP+)

Purification

Purification on EC 1.1.1.79 - glyoxylate reductase (NADP+)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, affinity chromatography, Sephadex G-75SF, AgdApP-4
-
centrifugation at 10500g, isoelectric focusing
Populus gelrica
-
ethanol precipitation, DEAE-cellulose, CM-cellulose, affinity chromatography
-
isoenzyme 1, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, hydroxylapatite
-
isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150, phosphocellulose
-
isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, phospho-cellulose
-
nickel affinity column chromatography; recombinant truncated protein using His-tag
recombinant C-terminally His9-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity
-
recombinant enzyme; recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, the His-tag is cleaved by thrombin followed by gel filtration, over 95% purity
-
recombinant protein from Escherichia coli
recombinant protein from Escherichia coli using His-tag
-