1.1.1.67: mannitol 2-dehydrogenase

This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase, go to the full flat file.

Reaction

D-mannitol
+
NAD+
=
D-fructose
+
NADH
+
H+

Synonyms

D-mannitol dehydrogenase, DSF1, Hxt13, Hxt15, Hxt16, Hxt17, M2DH, Man1, MAN2, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol dehydrogenase 1, mannitol dehydrogenase 2, mannitol transporter, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, YNR073C

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.67 mannitol 2-dehydrogenase

Crystallization

Crystallization on EC 1.1.1.67 - mannitol 2-dehydrogenase

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, binary complex of selenomethionine substituted proteins with NAD(H) and a ternary complex with NAD(H) and D-mannitol have been determined to resolutions of 1.7 and 1.8 A respectively
-
hanging drop vapor diffusion method, binary complex with NAD+ and ternary complex with NAD+ and D-mannitol have been determined to resolutions of 1.7 and 1.8 A and R-factors of 0.171 and 0.176 respectively
-
purified recombinant His-tagged enzyme, 10 mg/ml protein in 50 mM Tris-HCl, pH 8.5, room temperature, microbatch-under-oil method, mixing of equal volumes of protein and crystallization solutions, the latter containing 30% 2-methyl-2,4-pentanediol, and 0.1 M Na HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 3.3 A resolution, molecular-replacement
-