1.1.1.67: mannitol 2-dehydrogenase

This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase, go to the full flat file.

Reaction

D-mannitol
+
NAD+
=
D-fructose
+
NADH
+
H+

Synonyms

D-mannitol dehydrogenase, DSF1, Hxt13, Hxt15, Hxt16, Hxt17, M2DH, Man1, MAN2, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol dehydrogenase 1, mannitol dehydrogenase 2, mannitol transporter, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, YNR073C

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.67 mannitol 2-dehydrogenase

Purification

Purification on EC 1.1.1.67 - mannitol 2-dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by heat treatment at 85C and on Ni-NTA column; Ni-NTA agarose column chromatography
by Ni2+-NTA affinity chromatography
-
Co2+ chelating Sepharose column chromatography and Superdex 200 gel filtration
-
HisBind affinity chromatography
-
histidine-tagged recombinant protein, expressed in Escherichia coli
-
mutants purified to apparent homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein