Cloned (Comment) | Organism |
---|---|
gene mtdh, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, 10 mg/ml protein in 50 mM Tris-HCl, pH 8.5, room temperature, microbatch-under-oil method, mixing of equal volumes of protein and crystallization solutions, the latter containing 30% 2-methyl-2,4-pentanediol, and 0.1 M Na HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 3.3 A resolution, molecular-replacement | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene mtdh | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
mannitol dehydrogenase | - |
Thermotoga maritima |
MtDH | - |
Thermotoga maritima |