1.1.1.67: mannitol 2-dehydrogenase

This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase, go to the full flat file.

Reaction

D-mannitol
+
NAD+
=
D-fructose
+
NADH
+
H+

Synonyms

D-mannitol dehydrogenase, DSF1, Hxt13, Hxt15, Hxt16, Hxt17, M2DH, Man1, MAN2, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol dehydrogenase 1, mannitol dehydrogenase 2, mannitol transporter, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, YNR073C

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.67 mannitol 2-dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.67 - mannitol 2-dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, 1 month, 50% loss of activity
-
-16C, 0.02 M sodium acetate buffer pH 6.0, 1 mM 2-mercaptoethanol, several weeks
-
-20C, 10 mM dithiothreitol, 45 days
-
3C, crystalline enzyme in ammonium sulfate, 2 months
-
4C or 20C, enzyme loses approximately 60% of its specific activity after storage for 3 weeks in presence of 1 mM DTT, without DTT the same loss is observed after 2 days
-