1.1.1.67: mannitol 2-dehydrogenase

This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase, go to the full flat file.

Reaction

D-mannitol
+
NAD+
=
D-fructose
+
NADH
+
H+

Synonyms

D-mannitol dehydrogenase, DSF1, Hxt13, Hxt15, Hxt16, Hxt17, M2DH, Man1, MAN2, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol dehydrogenase 1, mannitol dehydrogenase 2, mannitol transporter, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, YNR073C

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.67 mannitol 2-dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.67 - mannitol 2-dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
cells grown on D-glucose, crude extracts
0.012
-
cells grown on D-mannitol + glucose, crude extracts
0.015
-
cells grown on D-ribose, crude extracts
0.05
-
41C, pH 9.0
0.243
-
cells grown on D-mannitol, crude extracts
14
-
crude extract, at 25C, using 100 mM Tris-HCl buffer, pH 7.1
33
-
after 6.7fold purification, at 25C, using 100 mM Tris-HCl buffer, pH 7.1
46
-
histidine-tagged recombinant protein
54
purified enzyme at 80C with D-fructose as the substrate and NADH as the cofactor; purified enzyme, at 80C with fructose as the substrate and NADH as the cofactor
63
-
MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate
68.3
-
recombinant protein
additional information
-
the activity for inositol, sorbitol, galactitol, ribitol, xylitol, arabitol and erythritol is very low