18.104.22.168: mannitol 2-dehydrogenase
This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase, go to the full flat file.
D-mannitol dehydrogenase, DSF1, Hxt13, Hxt15, Hxt16, Hxt17, M2DH, Man1, MAN2, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol dehydrogenase 1, mannitol dehydrogenase 2, mannitol transporter, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, YNR073C
Specific Activity on EC 22.214.171.124 - mannitol 2-dehydrogenase
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cells grown on D-glucose, crude extracts
cells grown on D-mannitol + glucose, crude extracts
cells grown on D-ribose, crude extracts
cells grown on D-mannitol, crude extracts
crude extract, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1
after 6.7fold purification, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1
histidine-tagged recombinant protein
purified enzyme at 80°C with D-fructose as the substrate and NADH as the cofactor; purified enzyme, at 80°C with fructose as the substrate and NADH as the cofactor
MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate
the activity for inositol, sorbitol, galactitol, ribitol, xylitol, arabitol and erythritol is very low