EC Number   |
General Stability |
Reference |
|---|
   2.7.1.107 | activation of tyrosine kinases is required for membrane stabilization of DGKalpha, phosphorylation of DGKalpha at Tyr335 appears to be essential for membrane localization |
701582 |
| 2.7.1.107 | DGKalpha is also activated when it binds to membranes |
702424 |
| 2.7.1.107 | enzyme activity is destroyed by trypsin, no protection by substrate |
640351 |
| 2.7.1.107 | enzyme is unstable in membrane extract, upon storage overnight at 4°C 90% of the activity is lost |
640354 |
| 2.7.1.107 | lipid activators stabilize the enzyme agisnt inactivation induced by diacylglycerol. Mg2+ and Mn2+ show only a small stabilization effect both in presence and in absence of 10 mol% phosphatidylglycerol |
640356 |
| 2.7.1.107 | microsomal activity is more unstable on storage at 0°C than the soluble enzyme |
640368 |
| 2.7.1.107 | the enzyme exhibits a mean half-life of approximately 5.1 h in cultured cells |
737399 |
| 2.7.1.107 | the enzyme is stabilized during prolonged incubations in the presence of phospholipid vesicles, in a diacylglycerol-independent manner |
737399 |
| 2.7.1.107 | the recombinant isoform DGKepsilon and a truncated version lacking the first 40 residues lose activity upon being stored, particularly upon freezing and thawing, which is minimized by the addition of glycerol. |
760582 |
| 2.7.1.107 | when cosolubilized with diacylglycerol in octylglucoside micelles, the enzyme undergoes rapid irreversible inactivation |
640352 |
