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Literature summary for 2.7.1.107 extracted from

  • Tu-Sekine, B.; Goldschmidt, H.; Petro, E.; Raben, D.
    Diacylglycerol kinase ?: regulation and stability. (2013), Adv. Biol. Regul., 53, 118-126.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information isozyme DGK-teta is dependent on an activating accessory protein containing a poly-basic region, the broadening of the pH profile of DGK-theta is also dependent on a PBR-containing activator Mus musculus
phosphatidylserine broadens the pH-dependent activity when the enzyme is assayed in cytosolic extracts. Phosphatidylserineconcentrates Mg2+ ions at the interface Mus musculus

General Stability

General Stability Organism
the enzyme exhibits a mean half-life of approximately 5.1 h in cultured cells Mus musculus
the enzyme is stabilized during prolonged incubations in the presence of phospholipid vesicles, in a diacylglycerol-independent manner Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by ATP, ADP, sodium pyrophosphate, and beta-glycerophosphate Mus musculus
R59022 is an effective inhibitor of purified DGK-theta in vitro and in vivo Mus musculus
R59949 is an effective inhibitor of purified DGK-theta in vitro and in vivo Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, activates. Phosphatidylserine concentrates Mg2+ ions at the interface Mus musculus
Zn2+ zinc can play a non-catalytic role in DGK regulation. Pre-incubation of DGK-theta with 0.1 mM zinc ions inhibits the enzyme at pH 6.0-7.5 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
native PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol Mus musculus
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q6P5E8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system isozyme DGK-theta shows a striking predominate localization in the nervous system Mus musculus
-
fibroblast
-
Mus musculus
-
Neuro-2a cell
-
Mus musculus
-
Swiss 3T3 cell
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
Mus musculus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
DGK-theta
-
Mus musculus
diacylglycerol kinase theta
-
Mus musculus
type V DGK
-
Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
the broadening of the pH profile of DGK-theta is also dependent on a PBR-containing activator Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus

General Information

General Information Comment Organism
physiological function the enzyme plays important roles is the nervous system Mus musculus