General Stability | Organism |
---|---|
enzyme activity is destroyed by trypsin, no protection by substrate | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | 0.5%, 30% inhibition | Gallus gallus | |
ethanol | 0.5%, 20% inhibition | Gallus gallus | |
GTP | - |
Gallus gallus | |
Nonidet | 0.5%, 45% inhibition | Gallus gallus | |
Triton N-101 | 0.5%, 45% inhibition | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
GTP | pH 6.6, 30°C | Gallus gallus | |
0.16 | - |
ATP | pH 6.6, 30°C | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microtubule | - |
Gallus gallus | 5874 | - |
soluble | - |
Gallus gallus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+ | Gallus gallus | |
Mg2+ | absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+. 50% stimulation by 0.4 mM Mg2+, maximal activity at 5-40 mM | Gallus gallus | |
Mn2+ | absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+. Mn2+ is more effective on the enzyme from microtubule than on the enzyme from supernatant | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the molecular weight of the peak fraction of enzyme from supernatant is 125000 Da and the average molecular weight is 160000 Da, suggesting that more than one species might be present | Gallus gallus |
75000 | - |
microtubular enzyme, sucrose density gradient centrifugation | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | muscle | Gallus gallus | - |
muscle | embryonic | Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dipalmitoyl-sn-glycerol + GTP | - |
Gallus gallus | GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-dipalmitoyl-sn-glycerol | - |
Gallus gallus | ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | 45 | preincubation of microtubules, 10 min, 50% loss of activity | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
enzyme from supernantant fraction | Gallus gallus |
6.4 | - |
enzyme from microtubule | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | pH 4.5: about 50% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from supernatant. pH 4.5: about 55% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from microtubuli | Gallus gallus |