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Literature summary for 2.7.1.107 extracted from

  • Daleo, G.R.; Piras, M.M.; Piras, R.
    Diglyceride kinase activity of microtubules. Characterization and comparison with the protein kinase and ATPase activities associated with vinblastine-isolated tubulin of chick embryonic muscles (1976), Eur. J. Biochem., 68, 339-346.
    View publication on PubMed

General Stability

General Stability Organism
enzyme activity is destroyed by trypsin, no protection by substrate Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
deoxycholate 0.5%, 30% inhibition Gallus gallus
ethanol 0.5%, 20% inhibition Gallus gallus
GTP
-
Gallus gallus
Nonidet 0.5%, 45% inhibition Gallus gallus
Triton N-101 0.5%, 45% inhibition Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
GTP pH 6.6, 30°C Gallus gallus
0.16
-
ATP pH 6.6, 30°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microtubule
-
Gallus gallus 5874
-
soluble
-
Gallus gallus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+ Gallus gallus
Mg2+ absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+. 50% stimulation by 0.4 mM Mg2+, maximal activity at 5-40 mM Gallus gallus
Mn2+ absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+. Mn2+ is more effective on the enzyme from microtubule than on the enzyme from supernatant Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the molecular weight of the peak fraction of enzyme from supernatant is 125000 Da and the average molecular weight is 160000 Da, suggesting that more than one species might be present Gallus gallus
75000
-
microtubular enzyme, sucrose density gradient centrifugation Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo muscle Gallus gallus
-
muscle embryonic Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-sn-glycerol + GTP
-
Gallus gallus GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-dipalmitoyl-sn-glycerol
-
Gallus gallus ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43 45 preincubation of microtubules, 10 min, 50% loss of activity Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
enzyme from supernantant fraction Gallus gallus
6.4
-
enzyme from microtubule Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 pH 4.5: about 50% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from supernatant. pH 4.5: about 55% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from microtubuli Gallus gallus