Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | purified enzyme is completely devoid of activity without addition of phospholipid or deoxycholate | Catharanthus roseus | |
Phospholipid | purified enzyme is completely devoid of activity without addition of phospholipid or deoxycholate | Catharanthus roseus |
General Stability | Organism |
---|---|
enzyme is unstable in membrane extract, upon storage overnight at 4°C 90% of the activity is lost | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Catharanthus roseus | |
Co2+ | - |
Catharanthus roseus | |
deoxycholate | 1 mM, about 50% inhibition | Catharanthus roseus | |
Triton X-100 | 0.5 mM, 50% inhibition | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ATP | pH 7.5, 25°C | Catharanthus roseus | |
0.25 | - |
1,2-diacyl-sn-glycerol | pH 7.5, 25°C | Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Catharanthus roseus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation is required for activity. Mg2+ is most effective, maximal activation at about 10 mM | Catharanthus roseus | |
Mn2+ | slight activation at 5 mM | Catharanthus roseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 51000, SDS-PAGE | Catharanthus roseus |
68000 | - |
less than, sucrose density gradient centrifugation | Catharanthus roseus |
250000 | - |
gel filtration | Catharanthus roseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Catharanthus roseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.231 | - |
- |
Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | - |
Catharanthus roseus | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | - |
Catharanthus roseus | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51000, SDS-PAGE | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Catharanthus roseus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | rapid decrease of activity below pH 6.5, pH 7: optimum | Catharanthus roseus |