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Literature summary for 2.7.1.107 extracted from

  • Wissing, J.; Heim, S.; Wagner, K.G.
    Diacylglycerol kinase from suspension cultured plant cells (1989), Plant Physiol., 90, 1546-1551.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate purified enzyme is completely devoid of activity without addition of phospholipid or deoxycholate Catharanthus roseus
Phospholipid purified enzyme is completely devoid of activity without addition of phospholipid or deoxycholate Catharanthus roseus

General Stability

General Stability Organism
enzyme is unstable in membrane extract, upon storage overnight at 4°C 90% of the activity is lost Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Catharanthus roseus
Co2+
-
Catharanthus roseus
deoxycholate 1 mM, about 50% inhibition Catharanthus roseus
Triton X-100 0.5 mM, 50% inhibition Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
ATP pH 7.5, 25°C Catharanthus roseus
0.25
-
1,2-diacyl-sn-glycerol pH 7.5, 25°C Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Catharanthus roseus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation is required for activity. Mg2+ is most effective, maximal activation at about 10 mM Catharanthus roseus
Mn2+ slight activation at 5 mM Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
1 * 51000, SDS-PAGE Catharanthus roseus
68000
-
less than, sucrose density gradient centrifugation Catharanthus roseus
250000
-
gel filtration Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Catharanthus roseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.231
-
-
Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
Catharanthus roseus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + sn-1,2-dioleoylglycerol
-
Catharanthus roseus ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Catharanthus roseus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7 rapid decrease of activity below pH 6.5, pH 7: optimum Catharanthus roseus