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Information on EC 4.2.1.108 - ectoine synthase
for references in articles please use BRENDA:EC4.2.1.108
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IUBMB Comments
Ectoine is an osmoprotectant that is found in halophilic eubacteria. This enzyme is part of the ectoine biosynthesis pathway and only acts in the direction of ectoine formation. cf. EC 3.5.4.44, ectoine hydrolase.
The enzyme appears in viruses and cellular organisms
- 4.2.1.108
-
halophil
-
diaminobutyric
- halomonas
- elongata
-
hydroxyectoine
-
osmoprotectant
-
ectabc
-
methanotrophs
-
halotolerant
-
l-2,4-diaminobutyric
- salexigens
-
methylomicrobium
-
osmoadaptation
- biotechnology
- industry
showSynonyms
ectoine synthase, l-ectoine synthase, saectc, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(Sa)Ect
EctC
ectoine synthase
L-ectoine synthase
SaEctC
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2S)-4-acetamido-2-aminobutanoate = L-ectoine + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
condensation
condensation
Halalkalibacterium halodurans TCJAR021
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
ectoine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
N4-acetyl-L-2,4-diaminobutanoate hydro-lyase (L-ectoine-forming)
Ectoine is an osmoprotectant that is found in halophilic eubacteria. This enzyme is part of the ectoine biosynthesis pathway and only acts in the direction of ectoine formation. cf. EC 3.5.4.44, ectoine hydrolase.
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CAS REGISTRY NUMBER
COMMENTARY
130457-09-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
Substrates: -
Products: -
Products: -
?
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
-
Substrates: -
Products: -
Products: -
?
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
Substrates: i.e. N-gamma-acetyl-L-2,4-diaminobutanoate
Products: -
Products: -
?
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Substrates: -
Products: -
Products: -
?
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Substrates: i.e. N-gamma-acetyl-L-2,4-diaminobutanoate
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR product identification
Products: NMR product identification
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR product identification
Products: NMR product identification
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine is the compatible solute in the cell
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
Products: -
Products: -
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR and thin layer chromatography product identification
Products: NMR and thin layer chromatography product identification
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine is the compatible solute in the cell
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR and TLC product identification
Products: NMR and TLC product identification
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR product identification
Products: NMR product identification
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR identification
Products: NMR identification
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR identification
Products: NMR identification
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR product identification
Products: NMR product identification
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
Products: NMR product identification
Products: NMR product identification
?
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
?
-
Substrates: -
Products: -
Products: -
r
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
?
-
Substrates: -
Products: -
Products: -
r
L-glutamine
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
-
Substrates: -
Products: -
Products: -
r
L-glutamine
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
-
Substrates: -
Products: -
Products: -
r
N-alpha-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: -
Products: -
Products: -
?
N-alpha-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: an intramolecular condensation reaction
Products: -
Products: -
r
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: an intramolecular condensation reaction
Products: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
Products: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
r
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: an intramolecular condensation reaction
Products: -
Products: -
r
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: an intramolecular condensation reaction
Products: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
Products: i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase
r
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
Halalkalibacterium halodurans
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
Halalkalibacterium halodurans TCJAR021
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutyrate
L-ectoine + H2O
Halalkalibacterium halodurans
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutyrate
L-ectoine + H2O
Halalkalibacterium halodurans TCJAR021
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
Products: -
Products: -
?
additional information
?
-
Substrates: EctC not only effectively converts its natural substrate N-gamma-acetyl-L-2,4-diaminobutyric acid into ectoine through a cyclocondensation reaction, but it can also use the isomer N-alpha-acetyl-L-2,4-diaminobutyric acid as its substrate, albeit with substantially reduced catalytic efficiency
Products: -
Products: -
?
additional information
?
-
-
Substrates: EctC not only effectively converts its natural substrate N-gamma-acetyl-L-2,4-diaminobutyric acid into ectoine through a cyclocondensation reaction, but it can also use the isomer N-alpha-acetyl-L-2,4-diaminobutyric acid as its substrate, albeit with substantially reduced catalytic efficiency
Products: -
Products: -
?
additional information
?
-
Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Substrates: EctC not only effectively converts its natural substrate N-gamma-acetyl-L-2,4-diaminobutyric acid into ectoine through a cyclocondensation reaction, but it can also use the isomer N-alpha-acetyl-L-2,4-diaminobutyric acid as its substrate, albeit with substantially reduced catalytic efficiency
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
Substrates: -
Products: -
Products: -
?
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
-
Substrates: -
Products: -
Products: -
?
(2S)-4-acetamido-2-aminobutanoate
L-ectoine + H2O
Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Substrates: -
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine is the compatible solute in the cell
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
Products: -
Products: -
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine is the compatible solute in the cell
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
r
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, pathway overview
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: an intramolecular condensation reaction
Products: -
Products: -
r
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
-
Substrates: an intramolecular condensation reaction
Products: -
Products: -
r
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
Halalkalibacterium halodurans
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
Halalkalibacterium halodurans TCJAR021
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutyrate
L-ectoine + H2O
Halalkalibacterium halodurans
Substrates: -
Products: -
Products: -
?
N4-acetyl-L-2,4-diaminobutyrate
L-ectoine + H2O
Halalkalibacterium halodurans TCJAR021
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
NaCl
additional information
additional information
-
the enzyme is not affected by Mg2+, Ca2+, Mn2+, Li+, K+, and NH4+
additional information
determination of metal content of recombinant SaEctC protein by ICP-MS. Zn2+ or Co2+ can only weakly substitute for Fe2+. No activation with Ni2+, Mn2+, Cu2+, and Fe3+ at 1 mM
additional information
-
determination of metal content of recombinant SaEctC protein by ICP-MS. Zn2+ or Co2+ can only weakly substitute for Fe2+. No activation with Ni2+, Mn2+, Cu2+, and Fe3+ at 1 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
high osmolarity induces the enzyme expression, requirements
-
additional information
high osmolarity induces the enzyme expression, requirements
-
additional information
high salinity/osmolarity of the medium induces enzyme expression
-
additional information
-
high salinity/osmolarity of the medium induces enzyme expression
-
additional information
enzyme expression is 12fold induced by NaCl at 1 M
-
additional information
high osmolarity induces the enzyme expression, requirements
-
additional information
-
high osmolarity induces the enzyme expression, requirements
-
additional information
-
high osmolarity induces the enzyme expression, requirements
-
additional information
-
high osmolarity induces the enzyme expression, requirements
-
additional information
-
high osmolarity induces the enzyme expression, requirements
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.9
(2S)-4-acetamido-2-aminobutanoate
pH 8.5, 15°C, recombinant enzyme
25.4
N-alpha-acetyl-L-2,4-diaminobutanoate
pH 8.5, 15°C, recombinant enzyme
8.4
4-N-acetyl-L-2,4-diaminobutanoate
-
pH 9.5, 15°C, in presence of 0.77 M NaCl
11
4-N-acetyl-L-2,4-diaminobutanoate
-
pH 9.5, 15°C, in presence of 0.05 M NaCl
additional information
additional information
Michaelis-Menten-kinetics
-
additional information
additional information
-
Michaelis-Menten-kinetics
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
7.2
(2S)-4-acetamido-2-aminobutanoate
pH 8.5, 15°C, recombinant enzyme
0.6
N-alpha-acetyl-L-2,4-diaminobutanoate
pH 8.5, 15°C, recombinant enzyme
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.47
(2S)-4-acetamido-2-aminobutanoate
pH 8.5, 15°C, recombinant enzyme
0.02
N-alpha-acetyl-L-2,4-diaminobutanoate
pH 8.5, 15°C, recombinant enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.16
-
purified recombinant enzyme, substrate is 4,5-dihydro-2-methylimidazole-4-carboxylate, hydrolytic reaction, pH 8.5, 21°C
0.3
-
purified recombinant enzyme, substrate is 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, hydrolytic reaction, pH 8.5, 21°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
15
20
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0 - 30
-
the temperature optimum shifts from 0°C to 30°C with increasing NaCl concentration
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
formerly Halomonas elongata, strain DSM 3043, gene ectC encoded in the ectABC gene cluster
SwissProt
brenda
Halalkalibacterium halodurans
Halalkalibacterium halodurans TCJAR021
i.e. Methylobacter alcaliphilus, gene ectC
UniProt
brenda
no activity in Aneurinibacillus aneurinilyticus DSM 5562
formerly Bacillus aneurinilyticus
-
-
brenda
strain DSM 33, gene ectC encoded in the ectABC gene cluster
SwissProt
brenda
Halalkalibacterium halodurans
the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
TrEMBL
brenda
Halalkalibacterium halodurans TCJAR021
the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
TrEMBL
brenda
ectABCask gene cluster sequence; 20Z, obligate methanotroph, gene ectC
SwissProt
brenda
i.e. Methylobacter alcaliphilus, gene ectC
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
cells are grown in an atmosphere of air-methane 1:1 in 1 M NaCl
brenda
Highest Expressing Human Cell Lines