BRENDA - Enzyme Database
show all sequences of 4.2.1.108

Cloning and heterologous expression of ectoine biosynthesis genes from Bacillus halodurans in Escherichia coli

Rajan, L.A.; Joseph, T.C.; Thampuran, N.; James, R.; Kumar, K.A.; Viswanathan, C.; Bansal, K.C.; Biotechnol. Lett. 30, 1403-1407 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
ectoine has importance in dermopharmacy as anti ageing agents in skin creams, as components of shampoo, for oral care and as adjuvants for vaccines
Bacillus halodurans
Cloned(Commentary)
Cloned (Commentary)
Organism
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30
Bacillus halodurans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15000
-
SDS-PAGE, calculated
Bacillus halodurans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
L-ectoine + H2O
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
L-ectoine + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bacillus halodurans
A5A5G3
the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
-
Bacillus halodurans TCJAR021
A5A5G3
the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
L-ectoine + H2O
-
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
L-ectoine + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ectoine synthase
-
Bacillus halodurans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Bacillus halodurans
calculated
-
4.93
Application (protein specific)
Application
Commentary
Organism
biotechnology
ectoine has importance in dermopharmacy as anti ageing agents in skin creams, as components of shampoo, for oral care and as adjuvants for vaccines
Bacillus halodurans
Cloned(Commentary) (protein specific)
Commentary
Organism
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30
Bacillus halodurans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15000
-
SDS-PAGE, calculated
Bacillus halodurans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
L-ectoine + H2O
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
L-ectoine + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
L-ectoine + H2O
-
-
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
L-ectoine + H2O
-
-
-
?
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Bacillus halodurans
calculated
-
4.93
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749081
Widderich
Biochemistry and crystal stru ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
PLoS ONE
11
e0151285
2016
-
-
1
1
26
-
-
3
-
2
-
2
-
7
-
-
1
-
-
-
-
-
8
2
5
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
26
-
-
-
-
3
-
2
-
2
-
-
-
1
-
-
-
-
8
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
746650
Kobus
Overproduction, crystallizati ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Acta Crystallogr. Sect. F
71
1027-1032
2015
-
-
1
1
-
-
-
-
-
-
1
2
-
7
-
-
1
-
-
-
-
-
2
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
713911
Witt
Unexpected property of ectoine ...
Halomonas elongata, Halomonas elongata DSM 2581
Appl. Microbiol. Biotechnol.
91
113-122
2011
-
-
1
-
1
-
-
1
-
-
1
2
-
5
-
-
1
-
-
-
2
-
11
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
2
-
11
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
729687
Reshetnikov
Diversity and phylogeny of the ...
Methylarcula marina, Methylarcula marina ML1, Methylobacter marinus, Methylobacter marinus 7C, Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum ML1, Methylomicrobium kenyense, Methylomicrobium kenyense AMO1, Methylophaga alcalica, Methylophaga alcalica M8, Methylophaga thalassica, Methylophaga thalassica ATCC 33146
Extremophiles
15
653-663
2011
-
-
6
-
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
690643
Rajan
Characterization and phylogene ...
Bacillus halodurans, Bacillus halodurans TCJAR021
Arch. Microbiol.
190
481-487
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
691437
Rajan
Cloning and heterologous expre ...
Bacillus halodurans, Bacillus halodurans TCJAR021
Biotechnol. Lett.
30
1403-1407
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum
Arch. Microbiol.
184
286-297
2006
1
-
1
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
1
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Bacillus alcalophilus, Chromohalobacter salexigens, no activity in Aneurinibacillus aneurinilyticus, no activity in Aneurinibacillus aneurinilyticus DSM 5562, no activity in Bacillus cereus, no activity in Bacillus cereus DSM 31, no activity in Bacillus circulans, no activity in Bacillus licheniformis, no activity in Bacillus megaterium, no activity in Bacillus subtilis, no activity in Bacillus subtilis JH642, no activity in Bacillus thuringiensis, no activity in Paenibacillus polymyxa, Sporosarcina pasteurii, Sporosarcina psychrophila, Virgibacillus pantothenticus, Virgibacillus salexigens
Appl. Environ. Microbiol.
68
772-783
2002
6
-
2
-
-
-
-
-
-
1
-
12
-
27
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
-
1
-
12
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663114
Nakayama
Ectoine, the compatible solute ...
Halomonas elongata, Halomonas elongata OUT30018, no activity in Nicotiana tabacum
Plant Physiol.
122
1239-1247
2000
-
-
1
-
1
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
2
-
2
2
3
-
5
-
-
1
-
-
-
1
-
6
1
-
3
1
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
2
2
3
-
-
-
1
-
-
1
-
6
1
3
1
1
-
1
-
-
1
-
-
-
-
-
-
662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
1
1
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640090
Peters
-
The biosynthesis of ectoine ...
Halomonas elongata, Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
2
-
-
4
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-