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Literature summary for 4.2.1.108 extracted from

  • Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.
    Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata (1999), J. Bacteriol., 181, 91-99.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.108

General Stability

General Stability Organism
NaCl stabilizes the enzyme at above 2 M Halomonas elongata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.4
-
 4-N-acetyl-L-2,4-diaminobutanoate pH 9.5, 15°C, in presence of 0.77 M NaCl Halomonas elongata
11
-
 4-N-acetyl-L-2,4-diaminobutanoate pH 9.5, 15°C, in presence of 0.05 M NaCl Halomonas elongata

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by Mg2+, Ca2+, Mn2+, Li+, K+, and NH4+ Halomonas elongata
NaCl required for activity, maximal activity at 0.5 M Halomonas elongata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, SDS-PAGE Halomonas elongata
35000
-
 gel filtration in presence of 2 M NaCl Halomonas elongata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-N-acetyl-L-2,4-diaminobutanoate Halomonas elongata final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate Halomonas elongata OUT300318 final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate Halomonas elongata OUT30018 final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Halomonas elongata
-
 formerly KS3
-
Halomonas elongata OUT30018
-
 formerly KS3
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and hydroxylapatite chromatography, in presence of 1 mM L-2,4-diaminobutanoate and over 2 M NaCl to homogeneity Halomonas elongata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
 purified enzyme Halomonas elongata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Halomonas elongata L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range Halomonas elongata L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Halomonas elongata OUT300318 L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range Halomonas elongata OUT300318 L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Halomonas elongata OUT30018 L-ectoine + H2O
-
 ?
4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range Halomonas elongata OUT30018 L-ectoine + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Halomonas elongata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
-
 10 in presence of 0.05 M NaCl Halomonas elongata
15
-
 in presence of 0.77 M NaCl Halomonas elongata
30
-
 in presence of 3.0 M NaCl Halomonas elongata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 30 the temperature optimum shifts from 0°C to 30°C with increasing NaCl concentration Halomonas elongata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 the enzyme is quite stable below 30°C in presence of 2 M NaCl Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Halomonas elongata

pI Value

Organism Comment pI Value Maximum pI Value
Halomonas elongata isoelectric focusing 4.4 4.2