BRENDA - Enzyme Database
show all sequences of 4.2.1.108

Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata

Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.; J. Bacteriol. 181, 91-99 (1999)

Data extracted from this reference:

General Stability
General Stability
Organism
NaCl stabilizes the enzyme at above 2 M
Halomonas elongata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8.4
-
4-N-acetyl-L-2,4-diaminobutanoate
pH 9.5, 15C, in presence of 0.77 M NaCl
Halomonas elongata
11
-
4-N-acetyl-L-2,4-diaminobutanoate
pH 9.5, 15C, in presence of 0.05 M NaCl
Halomonas elongata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme is not affected by Mg2+, Ca2+, Mn2+, Li+, K+, and NH4+
Halomonas elongata
NaCl
required for activity, maximal activity at 0.5 M
Halomonas elongata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19000
-
x * 19000, SDS-PAGE
Halomonas elongata
35000
-
gel filtration in presence of 2 M NaCl
Halomonas elongata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata OUT300318
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata OUT30018
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Halomonas elongata
-
formerly KS3
-
Halomonas elongata OUT30018
-
formerly KS3
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and hydroxylapatite chromatography, in presence of 1 mM L-2,4-diaminobutanoate and over 2 M NaCl to homogeneity
Halomonas elongata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
16
-
purified enzyme
Halomonas elongata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640091
Halomonas elongata
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
640091
Halomonas elongata
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640091
Halomonas elongata OUT300318
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
640091
Halomonas elongata OUT300318
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640091
Halomonas elongata OUT30018
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
640091
Halomonas elongata OUT30018
L-ectoine + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 19000, SDS-PAGE
Halomonas elongata
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
-
10
in presence of 0.05 M NaCl
Halomonas elongata
15
-
in presence of 0.77 M NaCl
Halomonas elongata
30
-
in presence of 3.0 M NaCl
Halomonas elongata
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
-
30
the temperature optimum shifts from 0C to 30C with increasing NaCl concentration
Halomonas elongata
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
the enzyme is quite stable below 30C in presence of 2 M NaCl
Halomonas elongata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Halomonas elongata
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Halomonas elongata
isoelectric focusing
4.4
4.2
General Stability (protein specific)
General Stability
Organism
NaCl stabilizes the enzyme at above 2 M
Halomonas elongata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8.4
-
4-N-acetyl-L-2,4-diaminobutanoate
pH 9.5, 15C, in presence of 0.77 M NaCl
Halomonas elongata
11
-
4-N-acetyl-L-2,4-diaminobutanoate
pH 9.5, 15C, in presence of 0.05 M NaCl
Halomonas elongata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme is not affected by Mg2+, Ca2+, Mn2+, Li+, K+, and NH4+
Halomonas elongata
NaCl
required for activity, maximal activity at 0.5 M
Halomonas elongata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19000
-
x * 19000, SDS-PAGE
Halomonas elongata
35000
-
gel filtration in presence of 2 M NaCl
Halomonas elongata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata OUT300318
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata OUT30018
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and hydroxylapatite chromatography, in presence of 1 mM L-2,4-diaminobutanoate and over 2 M NaCl to homogeneity
Halomonas elongata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
16
-
purified enzyme
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640091
Halomonas elongata
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
640091
Halomonas elongata
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640091
Halomonas elongata OUT300318
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
640091
Halomonas elongata OUT300318
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640091
Halomonas elongata OUT30018
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range
640091
Halomonas elongata OUT30018
L-ectoine + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 19000, SDS-PAGE
Halomonas elongata
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
-
10
in presence of 0.05 M NaCl
Halomonas elongata
15
-
in presence of 0.77 M NaCl
Halomonas elongata
30
-
in presence of 3.0 M NaCl
Halomonas elongata
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
-
30
the temperature optimum shifts from 0C to 30C with increasing NaCl concentration
Halomonas elongata
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
the enzyme is quite stable below 30C in presence of 2 M NaCl
Halomonas elongata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Halomonas elongata
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Halomonas elongata
isoelectric focusing
4.4
4.2
Other publictions for EC 4.2.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
756904
Anburajan
Functional characterization o ...
Alkalihalobacillus clausii, Alkalihalobacillus clausii NIOT-DSB04
Infect. Genet. Evol.
73
261-265
2019
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
749081
Widderich
Biochemistry and crystal stru ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
PLoS ONE
11
e0151285
2016
-
-
1
1
26
-
-
3
-
2
-
2
-
9
-
-
1
-
-
-
-
-
8
2
5
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
26
-
-
-
-
3
-
2
-
2
-
-
-
1
-
-
-
-
8
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
746650
Kobus
Overproduction, crystallizati ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Acta Crystallogr. Sect. F
71
1027-1032
2015
-
-
1
1
-
-
-
-
-
-
1
2
-
15
-
-
1
-
-
-
-
-
2
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
713911
Witt
Unexpected property of ectoine ...
Halomonas elongata, Halomonas elongata DSM 2581
Appl. Microbiol. Biotechnol.
91
113-122
2011
-
-
1
-
1
-
-
1
-
-
1
2
-
8
-
-
1
-
-
-
2
-
11
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
2
-
11
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
729687
Reshetnikov
Diversity and phylogeny of the ...
Methylophaga thalassica, Methylophaga alcalica, Methylobacter marinus, Methylomicrobium alcaliphilum, Methylarcula marina, Methylomicrobium kenyense, Methylobacter marinus 7C, Methylomicrobium kenyense AMO1, Methylophaga thalassica ATCC 33146, Methylomicrobium alcaliphilum ML1, Methylophaga alcalica M8, Methylarcula marina ML1
Extremophiles
15
653-663
2011
-
-
6
-
-
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
690643
Rajan
Characterization and phylogene ...
Alkalihalobacillus halodurans, Alkalihalobacillus halodurans TCJAR021
Arch. Microbiol.
190
481-487
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
691437
Rajan
Cloning and heterologous expre ...
Alkalihalobacillus halodurans, Alkalihalobacillus halodurans TCJAR021
Biotechnol. Lett.
30
1403-1407
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum
Arch. Microbiol.
184
286-297
2006
1
-
1
-
-
-
-
-
-
1
-
1
-
4
-
-
-
-
-
1
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Alkalihalobacillus alcalophilus, Virgibacillus pantothenticus, Sporosarcina psychrophila, no activity in Bacillus cereus, no activity in Bacillus circulans, no activity in Bacillus licheniformis, no activity in Bacillus megaterium, no activity in Bacillus subtilis, Virgibacillus salexigens, no activity in Bacillus thuringiensis, no activity in Aneurinibacillus aneurinilyticus, no activity in Paenibacillus polymyxa, Sporosarcina pasteurii, Chromohalobacter salexigens, no activity in Bacillus cereus DSM 31, no activity in Aneurinibacillus aneurinilyticus DSM 5562, no activity in Bacillus subtilis JH642
Appl. Environ. Microbiol.
68
772-783
2002
6
-
2
-
-
-
-
-
-
1
-
12
-
26
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
-
1
-
12
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663114
Nakayama
Ectoine, the compatible solute ...
Halomonas elongata, no activity in Nicotiana tabacum, Halomonas elongata OUT30018
Plant Physiol.
122
1239-1247
2000
-
-
1
-
1
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
2
-
2
2
3
-
5
-
-
1
-
-
-
1
-
6
1
-
3
1
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
2
2
3
-
-
-
1
-
-
1
-
6
1
3
1
1
-
1
-
-
1
-
-
-
-
-
-
662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
1
1
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640090
Peters
-
The biosynthesis of ectoine ...
Halorhodospira halochloris, Halomonas elongata
FEMS Microbiol. Lett.
71
157-162
1990
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
2
-
-
4
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-