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Literature summary for 4.2.1.108 extracted from

  • Rajan, L.A.; Joseph, T.C.; Thampuran, N.; James, R.; Chinnusamy, V.; Bansal, K.C.
    Characterization and phylogenetic analysis of ectoine biosynthesis genes from Bacillus halodurans (2008), Arch. Microbiol., 190, 481-487.
    View publication on PubMed

Application

Application Comment Organism
industry the engineered Escherichia coli strain has potential industrial application since it produces ectoine at high rates and can avoid the complex down streaming process associated with the conventional bioprocess Halalkalibacterium halodurans

Cloned(Commentary)

Cloned (Comment) Organism
strain TCJAR021, expression in Escherichia coli M15 (pREP4) Halalkalibacterium halodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
SDS-PAGE, calculated Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
?
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans
-
L-ectoine + H2O
-
?
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans TCJAR021
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
?
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans TCJAR021
-
L-ectoine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans A5A5G3
-
-
Halalkalibacterium halodurans TCJAR021 A5A5G3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N4-acetyl-L-2,4-diaminobutyrate
-
Halalkalibacterium halodurans 2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
Halalkalibacterium halodurans TCJAR021 2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
Halalkalibacterium halodurans L-ectoine + H2O
-
?
N4-acetyl-L-2,4-diaminobutyrate
-
Halalkalibacterium halodurans TCJAR021 L-ectoine + H2O
-
?

Synonyms

Synonyms Comment Organism
ectoine synthase
-
Halalkalibacterium halodurans

pI Value

Organism Comment pI Value Maximum pI Value
Halalkalibacterium halodurans calculated
-
4.93