BRENDA - Enzyme Database
show all sequences of 4.2.1.108

The biosynthesis of ectoine

Peters, P.; Galinski, E.A.; Trüper, H.G.; FEMS Microbiol. Lett. 71, 157-162 (1990)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
Halorhodospira halochloris
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
L-ectoine + H2O
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Halomonas elongata
-
extremely halophilic eubacterium, strain DSM 2581
-
Halorhodospira halochloris
-
extremely halophilic eubacterium, strain DSM 1059
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
photosynthetically grown cells
Halorhodospira halochloris
-
culture condition:glucose-grown cell
-
Halomonas elongata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640090
Halorhodospira halochloris
L-ectoine + H2O
-
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
640090
Halomonas elongata
L-ectoine + H2O
-
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halomonas elongata
L-ectoine + H2O
NMR and thin layer chromatography product identification
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halorhodospira halochloris
L-ectoine + H2O
NMR and TLC product identification
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Halomonas elongata
37
-
assay at
Halorhodospira halochloris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Halomonas elongata
9
-
assay at
Halorhodospira halochloris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
Halorhodospira halochloris
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
Halomonas elongata
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
L-ectoine + H2O
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
photosynthetically grown cells
Halorhodospira halochloris
-
culture condition:glucose-grown cell
-
Halomonas elongata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
640090
Halorhodospira halochloris
L-ectoine + H2O
-
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M
640090
Halomonas elongata
L-ectoine + H2O
-
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halomonas elongata
L-ectoine + H2O
NMR and thin layer chromatography product identification
-
-
r
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
640090
Halorhodospira halochloris
L-ectoine + H2O
NMR and TLC product identification
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Halomonas elongata
37
-
assay at
Halorhodospira halochloris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Halomonas elongata
9
-
assay at
Halorhodospira halochloris
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749081
Widderich
Biochemistry and crystal stru ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
PLoS ONE
11
e0151285
2016
-
-
1
1
26
-
-
3
-
2
-
2
-
7
-
-
1
-
-
-
-
-
8
2
5
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
26
-
-
-
-
3
-
2
-
2
-
-
-
1
-
-
-
-
8
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
746650
Kobus
Overproduction, crystallizati ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Acta Crystallogr. Sect. F
71
1027-1032
2015
-
-
1
1
-
-
-
-
-
-
1
2
-
7
-
-
1
-
-
-
-
-
2
1
4
-
-
-
-
-
-
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1
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1
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-
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1
2
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1
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
713911
Witt
Unexpected property of ectoine ...
Halomonas elongata, Halomonas elongata DSM 2581
Appl. Microbiol. Biotechnol.
91
113-122
2011
-
-
1
-
1
-
-
1
-
-
1
2
-
5
-
-
1
-
-
-
2
-
11
1
1
1
-
-
-
1
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-
-
-
-
-
-
-
1
-
-
1
-
-
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1
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1
2
-
-
-
1
-
-
2
-
11
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
729687
Reshetnikov
Diversity and phylogeny of the ...
Methylarcula marina, Methylarcula marina ML1, Methylobacter marinus, Methylobacter marinus 7C, Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum ML1, Methylomicrobium kenyense, Methylomicrobium kenyense AMO1, Methylophaga alcalica, Methylophaga alcalica M8, Methylophaga thalassica, Methylophaga thalassica ATCC 33146
Extremophiles
15
653-663
2011
-
-
6
-
-
-
-
-
-
-
-
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19
-
-
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6
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
690643
Rajan
Characterization and phylogene ...
Bacillus halodurans, Bacillus halodurans TCJAR021
Arch. Microbiol.
190
481-487
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
4
-
1
-
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1
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1
1
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1
4
-
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4
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
691437
Rajan
Cloning and heterologous expre ...
Bacillus halodurans, Bacillus halodurans TCJAR021
Biotechnol. Lett.
30
1403-1407
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
4
-
1
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-
-
-
-
-
-
-
-
1
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-
1
1
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-
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-
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1
4
-
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-
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4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum
Arch. Microbiol.
184
286-297
2006
1
-
1
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
1
-
-
2
-
1
1
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-
-
1
-
-
-
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-
1
-
1
-
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-
-
-
-
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1
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1
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-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Bacillus alcalophilus, Chromohalobacter salexigens, no activity in Aneurinibacillus aneurinilyticus, no activity in Aneurinibacillus aneurinilyticus DSM 5562, no activity in Bacillus cereus, no activity in Bacillus cereus DSM 31, no activity in Bacillus circulans, no activity in Bacillus licheniformis, no activity in Bacillus megaterium, no activity in Bacillus subtilis, no activity in Bacillus subtilis JH642, no activity in Bacillus thuringiensis, no activity in Paenibacillus polymyxa, Sporosarcina pasteurii, Sporosarcina psychrophila, Virgibacillus pantothenticus, Virgibacillus salexigens
Appl. Environ. Microbiol.
68
772-783
2002
6
-
2
-
-
-
-
-
-
1
-
12
-
27
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
-
1
-
12
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663114
Nakayama
Ectoine, the compatible solute ...
Halomonas elongata, Halomonas elongata OUT30018, no activity in Nicotiana tabacum
Plant Physiol.
122
1239-1247
2000
-
-
1
-
1
-
-
-
-
-
-
2
-
9
-
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-
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4
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1
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1
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2
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-
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4
-
-
-
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-
-
-
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-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
2
-
2
2
3
-
5
-
-
1
-
-
-
1
-
6
1
-
3
1
1
-
1
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1
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-
1
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2
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2
2
3
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1
-
-
1
-
6
1
3
1
1
-
1
-
-
1
-
-
-
-
-
-
662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
1
1
1
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2
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3
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3
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1
1
1
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3
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-
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640090
Peters
-
The biosynthesis of ectoine ...
Halomonas elongata, Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
-
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-
-
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2
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2
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