Literature summary for 4.2.1.108 extracted from

Reshetnikov, A.S.; Khmelenina, V.N.; Trotsenko, Y.A.
Characterization of the ectoine biosynthesis genes of haloalkalotolerant obligate methanotroph "Methylomicrobium alcaliphilum 20Z" (2006), Arch. Microbiol., 184, 286-297.

Search for more literature for 4.2.1.108

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	enzyme expression is 12fold induced by NaCl at 1 M	Methylotuvimicrobium alcaliphilum

Cloned(Commentary)

Cloned (Comment)	Organism
gene ectC located in the singel operon ectABCask, DNA sequence determination and analysis, genetic organization, expression in Escherichia coli strain X1-Blue	Methylotuvimicrobium alcaliphilum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	required for activity	Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-N-acetyl-L-2,4-diaminobutanoate	Methylotuvimicrobium alcaliphilum	final step in L-ectoine biosynthesis, pathway overview	L-ectoine + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylotuvimicrobium alcaliphilum	Q4JQJ3	ectABCask gene cluster sequence; 20Z, obligate methanotroph, gene ectC	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	cells are grown in an atmosphere of air-methane 1:1 in 1 M NaCl	Methylotuvimicrobium alcaliphilum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-N-acetyl-L-2,4-diaminobutanoate	final step in L-ectoine biosynthesis, pathway overview	Methylotuvimicrobium alcaliphilum	L-ectoine + H2O	-	?
4-N-acetyl-L-2,4-diaminobutanoate	i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid	Methylotuvimicrobium alcaliphilum	L-ectoine + H2O	NMR product identification	r

Synonyms

Synonyms	Comment	Organism
EctC	-	Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Methylotuvimicrobium alcaliphilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Methylotuvimicrobium alcaliphilum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)