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Literature summary for 4.2.1.108 extracted from
- Characterization of the ectoine biosynthesis genes of haloalkalotolerant obligate methanotroph "Methylomicrobium alcaliphilum 20Z" (2006), Arch. Microbiol., 184, 286-297.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme expression is 12fold induced by NaCl at 1 M | Methylotuvimicrobium alcaliphilum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ectC located in the singel operon ectABCask, DNA sequence determination and analysis, genetic organization, expression in Escherichia coli strain X1-Blue | Methylotuvimicrobium alcaliphilum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | required for activity | Methylotuvimicrobium alcaliphilum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-N-acetyl-L-2,4-diaminobutanoate | Methylotuvimicrobium alcaliphilum | final step in L-ectoine biosynthesis, pathway overview | L-ectoine + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylotuvimicrobium alcaliphilum | Q4JQJ3 | ectABCask gene cluster sequence; 20Z, obligate methanotroph, gene ectC | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cells are grown in an atmosphere of air-methane 1:1 in 1 M NaCl | Methylotuvimicrobium alcaliphilum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-N-acetyl-L-2,4-diaminobutanoate | final step in L-ectoine biosynthesis, pathway overview | Methylotuvimicrobium alcaliphilum | L-ectoine + H2O | - |
? | |
| 4-N-acetyl-L-2,4-diaminobutanoate | i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid | Methylotuvimicrobium alcaliphilum | L-ectoine + H2O | NMR product identification | r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EctC | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Methylotuvimicrobium alcaliphilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Methylotuvimicrobium alcaliphilum |
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