BRENDA - Enzyme Database
show all sequences of 4.2.1.108

Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli

Louis, P.; Galinski, E.A.; Microbiology 143, 1141-1149 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
high salinity/osmolarity of the medium induces enzyme expression
Marinococcus halophilus
Application
Application
Commentary
Organism
biotechnology
inducible recombinant enzyme expression in Escherichia coli confers the capability to grow on high concentration of osmolytes by increasing the osmotolerance via L-ectoine production
Marinococcus halophilus
Cloned(Commentary)
Cloned (Commentary)
Organism
gene ectC, DNA and amino acid sequence determination and analysis, genetic mapping and organization, functional expression in Escherichia coli, the recombinant enzyme expression is osmoregulated in response to medium salinity in Escherichia coli
Marinococcus halophilus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
Marinococcus halophilus
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
additional information
Marinococcus halophilus
the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Marinococcus halophilus
O06061
gene ectC
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
662773
Marinococcus halophilus
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
662773
Marinococcus halophilus
L-ectoine + H2O
-
-
-
?
additional information
the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
662773
Marinococcus halophilus
?
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
high salinity/osmolarity of the medium induces enzyme expression
Marinococcus halophilus
Application (protein specific)
Application
Commentary
Organism
biotechnology
inducible recombinant enzyme expression in Escherichia coli confers the capability to grow on high concentration of osmolytes by increasing the osmotolerance via L-ectoine production
Marinococcus halophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ectC, DNA and amino acid sequence determination and analysis, genetic mapping and organization, functional expression in Escherichia coli, the recombinant enzyme expression is osmoregulated in response to medium salinity in Escherichia coli
Marinococcus halophilus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
Marinococcus halophilus
final step in L-ectoine biosynthesis, pathway overview
L-ectoine + H2O
-
-
?
additional information
Marinococcus halophilus
the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-N-acetyl-L-2,4-diaminobutanoate
final step in L-ectoine biosynthesis, pathway overview
662773
Marinococcus halophilus
L-ectoine + H2O
-
-
-
?
4-N-acetyl-L-2,4-diaminobutanoate
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid
662773
Marinococcus halophilus
L-ectoine + H2O
-
-
-
?
additional information
the enzyme is osmoregulated in response to medium salinity allowing the organism to live in environments with high concentration of osmolytes
662773
Marinococcus halophilus
?
-
-
-
?
Other publictions for EC 4.2.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
756904
Anburajan
Functional characterization o ...
Alkalihalobacillus clausii, Alkalihalobacillus clausii NIOT-DSB04
Infect. Genet. Evol.
73
261-265
2019
-
-
1
-
-
-
-
-
-
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4
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1
1
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1
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1
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1
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2
2
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-
749081
Widderich
Biochemistry and crystal stru ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
PLoS ONE
11
e0151285
2016
-
-
1
1
26
-
-
3
-
2
-
2
-
9
-
-
1
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8
2
5
1
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2
1
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1
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1
26
-
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3
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2
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2
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1
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-
8
2
1
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-
2
1
-
-
-
-
2
2
-
2
2
746650
Kobus
Overproduction, crystallizati ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256
Acta Crystallogr. Sect. F
71
1027-1032
2015
-
-
1
1
-
-
-
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-
1
2
-
15
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1
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2
1
4
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1
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1
2
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1
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2
1
-
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-
-
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-
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2
2
-
-
-
713911
Witt
Unexpected property of ectoine ...
Halomonas elongata, Halomonas elongata DSM 2581
Appl. Microbiol. Biotechnol.
91
113-122
2011
-
-
1
-
1
-
-
1
-
-
1
2
-
8
-
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1
-
-
-
2
-
11
1
1
1
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1
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1
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1
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1
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1
2
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1
-
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2
-
11
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
729687
Reshetnikov
Diversity and phylogeny of the ...
Methylophaga thalassica, Methylophaga alcalica, Methylobacter marinus, Methylomicrobium alcaliphilum, Methylarcula marina, Methylomicrobium kenyense, Methylobacter marinus 7C, Methylomicrobium kenyense AMO1, Methylomicrobium alcaliphilum ML1, Methylophaga alcalica M8, Methylarcula marina ML1, Methylophaga thalassica ATCC 33146
Extremophiles
15
653-663
2011
-
-
6
-
-
-
-
-
-
-
-
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-
21
-
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-
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6
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-
-
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-
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6
6
-
-
-
690643
Rajan
Characterization and phylogene ...
Alkalihalobacillus halodurans, Alkalihalobacillus halodurans TCJAR021
Arch. Microbiol.
190
481-487
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
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4
-
1
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1
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1
1
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1
4
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4
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-
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-
1
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-
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-
691437
Rajan
Cloning and heterologous expre ...
Alkalihalobacillus halodurans, Alkalihalobacillus halodurans TCJAR021
Biotechnol. Lett.
30
1403-1407
2008
-
1
1
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
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4
-
1
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-
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1
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1
1
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1
4
-
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4
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-
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-
-
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-
1
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum
Arch. Microbiol.
184
286-297
2006
1
-
1
-
-
-
-
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-
1
-
1
-
4
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1
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2
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1
1
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1
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1
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1
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1
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1
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-
1
-
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2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Alkalihalobacillus alcalophilus, Virgibacillus pantothenticus, Sporosarcina psychrophila, no activity in Bacillus cereus, no activity in Bacillus circulans, no activity in Bacillus licheniformis, no activity in Bacillus megaterium, no activity in Bacillus subtilis, Virgibacillus salexigens, no activity in Bacillus thuringiensis, no activity in Aneurinibacillus aneurinilyticus, no activity in Paenibacillus polymyxa, Sporosarcina pasteurii, Chromohalobacter salexigens, no activity in Bacillus cereus DSM 31, no activity in Aneurinibacillus aneurinilyticus DSM 5562, no activity in Bacillus subtilis JH642
Appl. Environ. Microbiol.
68
772-783
2002
6
-
2
-
-
-
-
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1
-
12
-
27
-
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18
-
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6
-
2
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1
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12
-
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18
-
-
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-
-
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-
663114
Nakayama
Ectoine, the compatible solute ...
Halomonas elongata, no activity in Nicotiana tabacum, Halomonas elongata OUT30018
Plant Physiol.
122
1239-1247
2000
-
-
1
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1
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2
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9
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4
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1
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4
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640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
2
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2
2
3
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5
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1
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1
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6
1
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3
1
1
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1
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1
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1
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2
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2
2
3
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1
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1
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6
1
3
1
1
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1
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1
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662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
1
1
1
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640090
Peters
-
The biosynthesis of ectoine ...
Halorhodospira halochloris, Halomonas elongata
FEMS Microbiol. Lett.
71
157-162
1990
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