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Information on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase
for references in articles please use BRENDA:EC3.5.1.28
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EC Tree 3 Hydrolases
3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
3.5.1 In linear amides
3.5.1.28 N-acetylmuramoyl-L-alanine amidase
The enzyme appears in viruses and cellular organisms
- 3.5.1.28
- endolysins
- staphylococcus
- aureus
- autolysins
- streptococcus
- listeria
- pneumococcal
-
bacteriolytic
- murein
-
autolytic
-
holins
- prophage
- muropeptides
-
pgrps
-
wall-binding
-
teichoic
- lysostaphin
- transglycosylase
-
histidine-dependent
-
n-acetylmuramidase
-
choline-containing
-
bacteriophage-encoded
-
diaminopimelic
- muramidase
- siphoviridae
-
phage-encoded
- n-acetylglucosaminidase
-
endo-beta-n-acetylglucosaminidase
- lysodeikticus
- drug development
- agriculture
- diagnostics
Reaction Schemes
showSynonyms
n-acetylmuramoyl-l-alanine amidase, pglyrp2, cell wall hydrolase, t7 lysozyme, n-acetylmuramyl-l-alanine amidase, phage endolysin, namlaa, pgrp-l, cwlj1, amic2, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylmuramoyl-alanine amidase
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-
-
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acetylmuramyl-alanine amidase
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acetylmuramyl-L-alanine amidase
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AMI1
AmiA
AmiC
AmiC2
AmiE
AmpD
Autolysin
cell separation amidase
cell wall amidase
Cell wall hydrolase
CwlB protein
cwlC
EC 3.4.17.7
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-
formerly
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EC 3.4.19.10
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formerly
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endolysin
GC-AmiC
LysBPS13
lysostaphin
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activities: N-acetyl muramyl-L-alanine amidase, glycylglycine endopeptidase and endo-beta-N-acetyl glucosamidase
LytA
LytA-like N-acetylmuramoyl-L-alanine amidase
LytAB6
LytAHER
Lytic amidase
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-
-
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LytN
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LytN harbors LysM and histidine-dependent amidohydrolase/peptidase domains, the latter of which functions as both an N-acetylmuramoyl-L-alanine amidase and D-alanyl-glycine endopeptidase
MSMEG_6281
Mtb peptidoglycan amidase
Mucopeptide aminohydrolase
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-
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murein hydrolase
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-
-
-
MurnAc-lAA
N-acetyl-muramoyl-L-alanine amidase
N-acetylmuramic acid L-alanine amidase
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-
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N-acetylmuramoyl-L-alanine amidase
N-acetylmuramoyl-L-alanine amidase type I
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-
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N-acetylmuramoyl-L-alanine amidase type II
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N-acetylmuramyl-L-alanine amidase
N-acetylmuramylalanine amidase
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N-acylmuramyl-L-alanine amidase
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-
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NAM-amidase
NAMLAA
Npun_F1846
ORFL3
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-
-
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Peptidoglycan hydrolase
PG amidase
phage endolysin
RC0497
RS15875
Rv3717
Skl
T3 lysozyme
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-
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T7 lysozyme
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-
-
-
additional information
Autolysin
-
-
-
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Cell wall hydrolase
-
-
-
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endolysin
Bacillus cereus phage BPS13
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-
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LysBPS13
Bacillus cereus phage BPS13
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-
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N-acetylmuramoyl-L-alanine amidase
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-
-
-
N-acetylmuramoyl-L-alanine amidase
activity of a bifunctional autolysin
N-acetylmuramyl-L-alanine amidase
-
-
-
-
N-acetylmuramyl-L-alanine amidase
Bacillus cereus phage BPS13
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-
-
additional information
the enzyme belongs to the peptidoglycan recognition protein, PGRP, family
additional information
the enzyme belongs to the peptidoglycan recognition protein, PGRP, family
additional information
-
the enzyme is a member of the choline-binding family, subfamily CHAP-containing enzymes, of proteins
additional information
-
the enzyme is a member of the choline-binding family, subfamily CHAP-containing enzymes, of proteins
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala + H2O = N-acetylglucosaminyl-N-acetylmuramic acid + L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala + H2O = N-acetylglucosaminyl-N-acetylmuramic acid + L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
-
-
-
-
GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala + H2O = N-acetylglucosaminyl-N-acetylmuramic acid + L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
catalytic mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
peptidoglycan recycling I, peptidoglycan recycling II
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SYSTEMATIC NAME
IUBMB Comments
peptidoglycan amidohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
9013-25-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(GlcNAc-MurNAc-L-Ala-D-isoGln-L-Lys-D-Ala)2 + H2O
?
Substrates: -
Products: -
Products: -
?
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP + H2O
1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP
-
Substrates: -
Products: -
Products: -
?
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O
1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala
-
Substrates: -
Products: -
Products: -
?
1,6-anhydro-N-acetylmuramic acid-tripeptide + H2O
?
Substrates: -
Products: -
Products: -
?
1,6-anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys + H2O
1,6-anhydro-N-acetylmuramate + L-Ala-gamma-D-Glu-L-Lys
Substrates: -
Products: -
Products: -
?
1,6-anhydromuropeptide + H2O
?
-
Substrates: AmpD has a strict specificity for 1,6-anhydromuropeptides, three-dimensional structure, active site structure, catalytic mechanism, Lys-162 and Tyr-63 are probably involved in substrate binding
Products: -
Products: -
?
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O
?
-
Substrates: the substrate is likely not turned over in vitro by AmpD
Products: -
Products: -
?
7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGln-Lys(2,4-dinitrophenyl)-D-Ala-Arg + H2O
?
-
Substrates: -
Products: -
Products: -
?
7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGlu-Lys(2,4-dinitrophenyl)-D-Ala-Arg + H2O
?
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N,6-O-diacetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
beta-N-acetylglucosaminyl-(1-4)-N,6-O-diacetylmuramoate + L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N,6-O-diacetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine + H2O
?
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N,6-O-diacetylmuramyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
?
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoyl-L-Ala-D-isoglutaminyl-(L)-(beta-N-acetyl-glucosaminyl-(1-4)-N-acetylmuramoyl-L-Ala-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-Ala)-(D)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
?
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-(beta-N-acetyl-glucosaminyl-(1-4)-N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine)-(D)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoate + L-alanyl-D-isoglutaminyl-(L)-(beta-N-acetyl-glucosaminyl-(1-4)-N-acetylmuramoate + L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine)-(D)-meso-2,6-diaminopimelic acid-(D)-amide
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoate + L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine + H2O
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramoate + L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
?
-
Substrates: -
Products: -
Products: -
?
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine + H2O
beta-N-acetylglucosaminyl-(1-4)-N-acetylmuramic acid + L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine
-
Substrates: -
Products: -
Products: -
?
GlcNAc-anhMurNAc-L-Ala-D-Glu-Dap + H2O
L-Ala-D-Glu-Dap + GlcNAc-anhMurNAc
Substrates: -
Products: -
Products: -
?
GlcNAc-anhydroMurNAc-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala + H2O
GlcNAc-anhydroMurNAc + L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala
Substrates: biphasic behavior: rapid exponential phase preceding a linear phase (0.027 mM substrate, 0.000106 mM enzyme)
Products: -
Products: -
?
GlcNAc-MurNAc-anhydro-L-Ala-D-gluc-meso-diaminopimelyl-D-Ala + H2O
?
-
Substrates: -
Products: -
Products: -
?
GlcNAc-MurNAc-L-Ala-D-isoGIn-meso-diaminopimelyl-(D)-amide-(L)-D-Ala-D-Ala + H2O
?
-
Substrates: -
Products: -
Products: -
?
GlcNAc-MurNAc-L-Ala-D-isoGln-meso-diaminopimelyl-D-Ala + H2O
?
-
Substrates: -
Products: -
Products: -
?
GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala + H2O
N-acetylglucosaminyl-N-acetylmuramic acid + L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
murein + H2O
?
-
Substrates: also murein extracted from cells grown in the presence of penicillin, N-acetylated murein
Products: -
Products: -
?
MurNAc-L-Ala-D-isoGln-L-Lys + H2O
MurNAc + L-Ala-D-isoGln-L-Lys
Substrates: MurNAc-tripeptide, minimum peptidoglycan fragment hydrolyzed by PGRP-L
Products: -
Products: -
?
MurNAc-L-Ala-D-isoGln-L-Lys-D-Ala + H2O
MurNAc + L-Ala-D-isoGln-L-Lys-D-Ala
Substrates: -
Products: -
Products: -
?
N-(beta-1,4-N-acetylglucosaminyl-N-acetylmuramoyl-L-alanyl-gamma-D-isoglutaminyl)-L-lysyl-D-alanine + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetyl-glucosaminyl(beta1-4)N-acetylmuramoyl-tetrapeptide + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetyl-glucosaminyl(beta1-4)N-acetylmuramoyl-tripeptide + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylglucosaminyl-1,6-anhydro-N-acetylmuramyl-tripeptide + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine-gamma-D-glutamyl-mesodiaminopimelic acid + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide-(L)-D-alanine + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutaminyl-meso-diaminopimelic acid + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine + H2O
?
-
Substrates: -
Products: -
Products: -
?
phospho-N-acetylmuramoyl-L-alanyl-D-gamma-glutaminyl-meso-diaminopimelyl-D-alanyl-D-alanine + H2O
?
-
Substrates: -
Products: -
Products: -
?
uridine diphospho-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelic acid + H2O
?
-
Substrates: -
Products: -
Products: -
?
cell walls
?
-
Substrates: from Bacillus megaterium, Bacillus subtilis
Products: -
Products: -
?
cell walls
?
-
Substrates: from Bacillus subtilis, [14C] glucosamine-labeled
Products: -
Products: -
?
cell walls
?
-
Substrates: from Micrococcus luteus, Staphylococcus aureus, Bacillus megaterium
Products: -
Products: -
?
cell walls
?
-
Substrates: from Bacillus subtilis, [14C] glucosamine-labeled
Products: -
Products: -
?
cell walls
?
-
Substrates: from Micrococcus luteus, Staphylococcus aureus, Bacillus megaterium
Products: -
Products: -
?
cell walls
?
Dubowvirus dv11
-
Substrates: cell wall anchor structures from staphylococcal surface proteins
Products: -
Products: -
?
cell walls
?
-
Substrates: from Micrococcus luteus, Listeria monocytogenes
Products: -
Products: -
?
cell walls
?
Pneumococcal bacteriophage HB-3
-
Substrates: pneumococcal cell walls, labeled with [methyl-3H]choline, L-[4,5-3H]lysine monohydrochloride, or [2-14C]ethanolamine
Products: -
Products: -
?
cell walls
?
-
Substrates: from Bacillus megaterium, Bacillus subtilis
Products: -
Products: -
?
cell walls
?
-
Substrates: from Micrococcus luteus ATCC 46898
Products: -
Products: -
?
cell walls
?
-
Substrates: from Micrococcus luteus ATCC 46898
Products: -
Products: -
?
cell walls
?
-
Substrates: pneumococcal cell walls labeled with [methyl-3H] or [3H]lysine
Products: -
Products: -
?
cell walls
?
-
Substrates: from Diplococcus pneumoniae
Products: -
Products: -
?
GlcNAc-MurNAc(pentapeptide)-GlcNAc-MurNAc + H2O
?
-
Substrates: the cell separation amidase can utilize the small synthetic peptidoglycan a pentapeptide fragment as substrate. AmiC can cleave one peptide stem from synthetic PG dimer
Products: -
Products: -
?
GlcNAc-MurNAc(pentapeptide)-GlcNAc-MurNAc + H2O
?
-
Substrates: the cell separation amidase can utilize the small synthetic peptidoglycan a pentapeptide fragment as substrate. AmiC can cleave one peptide stem from synthetic PG dimer
Products: -
Products: -
?
GlcNAc-MurNAc(pentapeptide)-GlcNAc-MurNAc(pentapeptide) + H2O
?
-
Substrates: small synthetic peptidoglycan fragment
Products: -
Products: -
?
GlcNAc-MurNAc(pentapeptide)-GlcNAc-MurNAc(pentapeptide) + H2O
?
-
Substrates: small synthetic peptidoglycan fragment
Products: -
Products: -
?
GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala + H2O
N-acetylglucosaminyl-N-acetylmuramic acid + L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
-
Substrates: -
Products: -
Products: -
?
GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala + H2O
N-acetylglucosaminyl-N-acetylmuramic acid + L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
-
Substrates: -
Products: -
Products: -
?
MurNAc-L-Ala-D-isoGln + H2O
N-acetylmuramic acid + L-alanyl-iso-D-glutamine
Substrates: -
Products: -
Products: -
?
MurNAc-L-Ala-D-isoGln + H2O
N-acetylmuramic acid + L-alanyl-iso-D-glutamine
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Bacillus cereus phage
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Bacillus cereus phage BPS13
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Substrates: the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, the immunostimulatory properties of PGRP-SC1B-degraded peptidoglycan are highly reduced
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Substrates: the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrate from Escherichia coli, PGRP-SB1 is highly active against peptidoglycans that have a diaminopimelic acid residue in the cross-linking peptide, but lacks activity to most lysine-containing peptidoglycans
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Substrates: the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrates from Staphylococcus aureus, Micrococcus luteus, and Bacillus megaterium, the enzyme hydrolyzes the lactylamide bond between the glycan strand and the cross-linking peptides, analysis of the cleavage products by mass spectrometry
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
Substrates: the enzyme hydrolyzes bacterial peptidoglycan
Products: -
Products: -
?
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
-
Substrates: the enzyme performs cell wall lysis by cleavage of N-acetylmuramyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-D-gamma-glutaminyl-meso-diaminopimelic acid + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-D-gamma-glutaminyl-meso-diaminopimelic acid + H2O
?
-
Substrates: -
Products: -
Products: -
?
N-acetylmuramoyl-L-alanyl-D-gamma-glutaminyl-meso-diaminopimelic acid + H2O
?
-
Substrates: N-acetylmuramoyl-L-alanyl-D-gamma-glutaminyl-meso-diamino [3,4,5-3H]pimelic acid (3H-MTP)
Products: -
Products: -
?
peptidoglucan + H2O
?
Substrates: ChwA primarily hydrolyzes the N-acetylmuramoyl-L-alanyl amide bond, splits the linkage between polysaccharides and peptides, bacteriolytic/cell wall hydrolytic amidase, CwhA lyses CHCl3-treated Escherichia coli JM109 most efficiently, followed by Micrococcus luteus, Staphylococcus aureus IFO 13276, Enterococcus faecalis IFO 3971, Pediococcus acidilactici IFO3385 and intact Escherichia coli JM109
Products: -
Products: -
?
peptidoglucan + H2O
?
Substrates: PGRP-L hydrolyzes the amide bond between MurNAc and L-Ala of peptidoglycan, Cys-419 is required for the amidase activity, polymeric soluble uncross-linked peptidoglycan from Staphylococcus aureus, digest products
Products: -
Products: -
?
peptidoglucan + H2O
?
Substrates: cell autolysis, LytA rules the self-destruction of pneumococcal cells through degradation of their peptidoglycan backbone, LytA is an important pneumococcal virulence factor
Products: -
Products: -
?
peptidoglucan + H2O
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Substrates: LytA structure, 36.6 kDa modular enzyme comprising an N-terminal catalytic domain plus the C-terminal choline-binding domain, the former catalyzes the hydrolysis of the N-acetylmuramoyl-L-alanine bond present in the pneumococcal peptidoglycan backbone, fundamental role of the 11 C-terminal residues in the catalytic activity of LytA
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peptidoglycan + H2O
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Substrates: enzyme is required for the recognition of peptidoglycan, a component of bacterial cell walls, and therefore critical for the immune response
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peptidoglycan + H2O
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Substrates: the enzyme can act on macromolecular peptidoglycan to liberate cross-linked and non-cross-linked peptides
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peptidoglycan + H2O
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Substrates: the enzyme can act on macromolecular peptidoglycan to liberate cross-linked and non-cross-linked peptides
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peptidoglycan + H2O
peptidoglycan-derived fragment peptides
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Substrates: enzyme AmiC displays high basal activity on peptidoglycan. In vivo assay of HA-tagged recombinant enzyme in Escherichia coli cells, usage of fluorescein-labeled peptidoglycan. Digesting gonococcal PG with AmiC results in the recovery of soluble fragments of predicted masses matching tripeptide stem (L-Ala-gamma-D-Glu-mDAP), tetrapeptide stem (L-Ala-gamma-D-Glu-mDAP-D-Ala), pentapeptide stem (L-Ala-gamma-D-Glu-mDAPD-Ala-D-Ala), cross-linked tetrapeptide-tripeptide (4-3), crosslinked tetrapeptide-tetrapeptide (4-4), and cross-linked tetrapeptide-tetrapeptide-tetrapeptide (4-4-4). Substrate specificity of wild-type and mutant enzymes and product release analysis, detailed overview
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peptidoglycan + H2O
peptidoglycan-derived fragment peptides
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Substrates: enzyme AmiC displays high basal activity on peptidoglycan. In vivo assay of HA-tagged recombinant enzyme in Escherichia coli cells, usage of fluorescein-labeled peptidoglycan. Digesting gonococcal PG with AmiC results in the recovery of soluble fragments of predicted masses matching tripeptide stem (L-Ala-gamma-D-Glu-mDAP), tetrapeptide stem (L-Ala-gamma-D-Glu-mDAP-D-Ala), pentapeptide stem (L-Ala-gamma-D-Glu-mDAPD-Ala-D-Ala), cross-linked tetrapeptide-tripeptide (4-3), crosslinked tetrapeptide-tetrapeptide (4-4), and cross-linked tetrapeptide-tetrapeptide-tetrapeptide (4-4-4). Substrate specificity of wild-type and mutant enzymes and product release analysis, detailed overview
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additional information
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Substrates: bacteriolytic/cell wall hydrolytic amidase
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additional information
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Substrates: bacteriolytic/cell wall hydrolytic amidase
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additional information
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A0A1J9W3F9
Substrates: recombinant enzyme CwlJ1 shows activity against the spore cortical fragments of Bacillus anthracis when added exogenously. CwlJ1 is active on both decoated spores and spore cortical fragments. The Bacillus spore cortex is composed of a thick layer of peptidoglycan (PG) containing alternating beta-1,4 linked N-acetylglucosamine (NAG), N-acetylmuramic acid (NAM), and muramic acid-delta-lactam (MAL) residues, with NAM residues attached to either tetrapeptide (TP) or a single L-Ala residue
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additional information
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Substrates: recombinant enzyme CwlJ1 shows activity against the spore cortical fragments of Bacillus anthracis when added exogenously. CwlJ1 is active on both decoated spores and spore cortical fragments. The Bacillus spore cortex is composed of a thick layer of peptidoglycan (PG) containing alternating beta-1,4 linked N-acetylglucosamine (NAG), N-acetylmuramic acid (NAM), and muramic acid-delta-lactam (MAL) residues, with NAM residues attached to either tetrapeptide (TP) or a single L-Ala residue
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additional information
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A0A1J9W3F9
Substrates: CwlJ1 mainly recognizes large segments of glycan chains in the cortex instead of the minimal structural unit tetrasaccharide, with specificity for muramic acid-delta-lactam-containing glycan chains and preference for the tetrapeptide side chain. The enzyme cleaves Bacillus anthracis spore cortex peptidoglycan, structure, and specificity of CwlJ1 on spore core fragements, overview. Mass spectrometry analysis of muropeptides released from digested spore cortical fragments
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additional information
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Substrates: CwlJ1 mainly recognizes large segments of glycan chains in the cortex instead of the minimal structural unit tetrasaccharide, with specificity for muramic acid-delta-lactam-containing glycan chains and preference for the tetrapeptide side chain. The enzyme cleaves Bacillus anthracis spore cortex peptidoglycan, structure, and specificity of CwlJ1 on spore core fragements, overview. Mass spectrometry analysis of muropeptides released from digested spore cortical fragments
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additional information
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Substrates: the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival
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additional information
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Substrates: the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival
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additional information
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Substrates: the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival
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additional information
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Substrates: AmiE hydrolyzes the N-acetylmuramoyl-L-Ala bond of N-acetylmuramic acid peptides releasing N-acetylglucosamine, N-acetylmuramic acid, and peptides, but does not hydrolyze this bond of muropeptides containing N-acetylglucosamine at the nonreducing end
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