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Literature summary for 3.5.1.28 extracted from

  • Lee, M.; Zhang, W.; Hesek, D.; Noll, B.C.; Boggess, B.; Mobashery, S.
    Bacterial AmpD at the crossroads of peptidoglycan recycling and manifestation of antibiotic resistance (2009), J. Am. Chem. Soc., 131, 8742-8743.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP at pH 7.0, temperature not specified in the publication Citrobacter freundii
0.5
-
 1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala at pH 7.0, temperature not specified in the publication Citrobacter freundii
1.76
-
 4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala at pH 7.0, temperature not specified in the publication Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP + H2O Citrobacter freundii
-
 1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP
-
 ?
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O Citrobacter freundii
-
 1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala
-
 ?
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O Citrobacter freundii the substrate is likely not turned over in vitro by AmpD ?
-
 ?
bacterial peptidoglycan + H2O Citrobacter freundii
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
 strain ATCC6879
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP + H2O
-
 Citrobacter freundii 1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP
-
 ?
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O
-
 Citrobacter freundii 1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala
-
 ?
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O the substrate is likely not turned over in vitro by AmpD Citrobacter freundii ?
-
 ?
bacterial peptidoglycan + H2O
-
 Citrobacter freundii ?
-
 ?

Synonyms

Synonyms Comment Organism
AmpD
-
 Citrobacter freundii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala at pH 7.0, temperature not specified in the publication Citrobacter freundii
11
-
 1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala at pH 7.0, temperature not specified in the publication Citrobacter freundii
25
-
 1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP at pH 7.0, temperature not specified in the publication Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
 4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala at pH 7.0, temperature not specified in the publication Citrobacter freundii
22
-
 1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala at pH 7.0, temperature not specified in the publication Citrobacter freundii
69
-
 1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP at pH 7.0, temperature not specified in the publication Citrobacter freundii