Cloned (Comment) | Organism |
---|---|
expression in COS-7 cells and in HepG2/C3A human hepatoblastoma cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C419A | inactive mutant | Homo sapiens |
C530S | inactive mutant | Homo sapiens |
H411A | mutant with full amidase activity | Homo sapiens |
H436A | mutant with full amidase activity | Homo sapiens |
additional information | N-terminal DELTA1-343 deletion mutant of PGRP-L with reduced activity, inactive C-terminal DELTA344-576 deletion mutant of PGRP-L | Homo sapiens |
W442A | mutant with reduced amidase activity | Homo sapiens |
Y447A | inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition, restored by 1 mM Zn2+, partially restored by 10 mM Mg2+ | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | recombinant PGRP-L shows primarily intracellular and cell surface location | Homo sapiens | 9986 | - |
intracellular | recombinant PGRP-L shows primarily intracellular and cell surface location | Homo sapiens | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+-dependent | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96PD5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | PGRP-L, mainly in | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GlcNAc-MurNAc-L-Ala-D-isoGln-L-Lys-D-Ala)2 + H2O | - |
Homo sapiens | ? | - |
? | |
biotin-peptidoglucan + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | PGRP-L has no direct bacteriolytic activity for intact bacteria, not: MurNAc-L-Ala-D-isoGln, GlcNAc-MurNAc-L-Ala-D-isoGln | Homo sapiens | ? | - |
? | |
MurNAc-L-Ala-D-isoGln-L-Lys + H2O | MurNAc-tripeptide, minimum peptidoglycan fragment hydrolyzed by PGRP-L | Homo sapiens | MurNAc + L-Ala-D-isoGln-L-Lys | - |
? | |
MurNAc-L-Ala-D-isoGln-L-Lys-D-Ala + H2O | - |
Homo sapiens | MurNAc + L-Ala-D-isoGln-L-Lys-D-Ala | - |
? | |
peptidoglucan + H2O | PGRP-L hydrolyzes the amide bond between MurNAc and L-Ala of peptidoglycan, Cys-419 is required for the amidase activity, polymeric soluble uncross-linked peptidoglycan from Staphylococcus aureus, digest products | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidoglycan recognition protein-L | - |
Homo sapiens |
PGRP-L | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |