Literature summary for 3.5.1.28 extracted from

Oliveira, A.; Leite, M.; Kluskens, L.D.; Santos, S.B.; Melo, L.D.; Azeredo, J.
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high potential to control american foulbrood (2015), PLoS ONE, 10, e0132095 .

Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment)	Organism
gene IBBPl23_21, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged in Escherichia coli strains JM109 and BL21(DE3)	Paenibacillus phage phiIBB_P123

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	no signal peptide is identified	Paenibacillus phage phiIBB_P123	-	-

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus phage phiIBB_P123	R9VY83	i.e. Paenibacillus larvae phage phiIBB_Pl23 or Paenibacillus larvae bacteriophage	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged from Escherichia coli strains JM109 and BL21(DE3) by nickel affinity chromatography and ultrafiltration	Paenibacillus phage phiIBB_P123

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the Paenibacillus larvae strain Pl02-23 is used as the host for phiIBB_Pl23 amplification	Paenibacillus phage phiIBB_P123	-

Subunits

Subunits	Comment	Organism
?	x * 25800, about, sequence calculation, x * 25000, SDS-PAGE	Paenibacillus phage phiIBB_P123

Synonyms

Synonyms	Comment	Organism
endolysin	-	Paenibacillus phage phiIBB_P123
PlyPl23	-	Paenibacillus phage phiIBB_P123

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Paenibacillus phage phiIBB_P123

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Paenibacillus phage phiIBB_P123

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	over 70% of maximal activity	Paenibacillus phage phiIBB_P123

pI Value

Organism	Comment	pI Value Maximum	pI Value
Paenibacillus phage phiIBB_P123	sequence calculation	-	6.08

General Information

General Information	Comment	Organism
evolution	the enzyme's the C-terminus does not display homology to any identifiable domain	Paenibacillus phage phiIBB_P123
additional information	the enzyme has an N-acetylmuramoyl-L-alanine amidase catalytic domain and exhibits a broad-spectrum activity against common Paenibacillus larvae genotypes. Identification of five putative amidase catalytic residues: His29, His129, Phe53, Lys135, and Cys137	Paenibacillus phage phiIBB_P123
physiological function	phage phiIBB_Pl23 is able to lyse 16 of the 20 strains (80%), its endolysin is active against all Paenibacillus larvae strains tested, but Bacillus and Lactobacillus strains are not lysed by the phage or by the enzyme, overview	Paenibacillus phage phiIBB_P123

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Release 2023.1 (January 2023)