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Literature summary for 3.5.1.28 extracted from

  • Mellroth, P.; Steiner, H.
    PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity (2006), Biochem. Biophys. Res. Commun., 350, 994-999.
    View publication on PubMed
Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment) Organism
gene PGRP-SB1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of his6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inactivation through depletion of zinc ions Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ absolutely required for activity Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q70PY2 gene PGRP-SB1 or CG9681; gene PGRP-SB1 or CG9681
-

Source Tissue

Source Tissue Comment Organism Textmining
S2 cell
-
 Drosophila melanogaster
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium Drosophila melanogaster ?
-
 ?
N-acetylmuramoyl-L-alanine + H2O the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrate from Escherichia coli, PGRP-SB1 is highly active against peptidoglycans that have a diaminopimelic acid residue in the cross-linking peptide, but lacks activity to most lysine-containing peptidoglycans Drosophila melanogaster N-acetylmuramate + L-alanine
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the peptidoglycan recognition protein, PGRP, family Drosophila melanogaster
PGRP-SB1
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 Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 25 assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Drosophila melanogaster