Literature summary for 3.5.1.28 extracted from

Buettner, F.M.; Faulhaber, K.; Forchhammer, K.; Maldener, I.; Stehle, T.
Enabling cell-cell communication via nanopore formation structure, function and localization of the unique cell wall amidase AmiC2 of Nostoc punctiforme (2016), FEBS J., 283, 1336-1350 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene amiC, functional recombinant expression of GST-tagged wild-type enzyme and isolated catalytic domain in Escherichia coli strain MG165	Nostoc punctiforme

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant catalytic domain AmiC2-cat, X-ray diffraction structure determination and analysis at 1.12 A resolution	Nostoc punctiforme

Protein Variants

Protein Variants	Comment	Organism
additional information	none of the Glu578-variants shows enzyme activity, suggesting that this residue is indispensable for catalysis	Nostoc punctiforme

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Nostoc punctiforme	5618	-
additional information	native AmiC2 localizes to the maturing septum	Nostoc punctiforme	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the active site harbors an essential zinc ion, residues H447, E462 and H515 coordinate a zinc ion and mark the center of the active site	Nostoc punctiforme

Organism

Organism	UniProt	Comment	Textmining
Nostoc punctiforme	B2J2S4	-	-
Nostoc punctiforme ATCC 29133	B2J2S4	-	-

Synonyms

Synonyms	Comment	Organism
AmiC2	-	Nostoc punctiforme
cell wall amidase	-	Nostoc punctiforme
Npun_F1846	-	Nostoc punctiforme

General Information

General Information	Comment	Organism
additional information	overall structure and active site architecture of the enzyme's catalytic domain, AmiC2-cat, overview. AmiC2-cat adopts a mixed alphabeta-fold that is characteristic for Amidase_3 enzymes. Residues H447, E462, and H515 coordinate a zinc ion and mark the center of the active site. The conserved GHGG motif of residues Gly446, His447, Gly448, and Gly449 after strand beta1 permits two close turns and the tight folding at the top of the active site by the absence of side chains. Residue Glu578 is indispensable for catalysis	Nostoc punctiforme
physiological function	to orchestrate a complex life style in changing environments, the filamentous cyanobacterium Nostoc punctiforme facilitates communication between neighboring cells through septal junction complexes. This is achieved by nanopores that perforate the peptidoglycan (PGN) layer and traverse the cell septa. The N-acetylmuramoyl-L-alanine amidase AmiC2 (Npun_F1846) in Nostoc punctiforme generates arrays of such nanopores in the septal PGN, in contrast to homologous amidases that mediate daughter cell separation after cell division in unicellular bacteria	Nostoc punctiforme

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Release 2023.1 (January 2023)