Cloned (Comment) | Organism |
---|---|
gene PGRP-SB1, expression of His6-tagged wild-type and mutant enzyme | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
C168A | site-directed mutagenesis, the mutant is enzymatically inactive but retains its peptidoglycan affinity | Drosophila melanogaster |
C168S | site-directed mutagenesis, the mutant is enzymatically inactive but retains its peptidoglycan affinity | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
teichoic acid | - |
Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | absolutely required for activity, mutant PGRP-SC1B lacking a potential zinc ligand is enzymatically inactive but retains its peptidoglycan affinity | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylmuramoyl-L-alanine + H2O | Drosophila melanogaster | the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, the immunostimulatory properties of PGRP-SC1B-degraded peptidoglycan are highly reduced | N-acetylmuramate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q70PY2 | gene PGRP-SB1 or CG9681; gene PGRP-SB1 or CG9681 | - |
Purification (Comment) | Organism |
---|---|
gene PGRP-SB1, recombinant His6-tagged wild-type and mutant enzyme by nickel affinity chromatography | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme shows highest activity with peptidoglycan substrates from Staphylococcus aureus, and 34% and 23% of this activity with substrate from Micrococcus luteus and Bacillus megaterium, respectively, at pH 8.0 | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not show antibacterial activity | Drosophila melanogaster | ? | - |
? | |
N-acetylmuramoyl-L-alanine + H2O | the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, the immunostimulatory properties of PGRP-SC1B-degraded peptidoglycan are highly reduced | Drosophila melanogaster | N-acetylmuramate + L-alanine | - |
? | |
N-acetylmuramoyl-L-alanine + H2O | the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrates from Staphylococcus aureus, Micrococcus luteus, and Bacillus megaterium, the enzyme hydrolyzes the lactylamide bond between the glycan strand and the cross-linking peptides, analysis of the cleavage products by mass spectrometry | Drosophila melanogaster | N-acetylmuramate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the peptidoglycan recognition protein, PGRP, family | Drosophila melanogaster |
PGRP-SB1 | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Drosophila melanogaster |