Cloned (Comment) | Organism |
---|---|
gene SA97_036, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged cell wall binding domain CBD fused to GFP in Escherichia coli strain BL21(DE3) | Staphylococcus phage SA97 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | the enzyme is stable up to 300 mM and the activity only slightly affected, best without NaCl, 20% inhibition at 300 mM | Staphylococcus phage SA97 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | the enzyme contains a cell wall binding domain | Staphylococcus phage SA97 | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus phage SA97 | A0A0F6N3N1 | i.e. Staphylococcus aureus phage SA97 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged cell wall binding domain CBD fused to GFP from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to near homogeneity | Staphylococcus phage SA97 |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme LysSA97 structure reveals a putative cell wall binding domain (CBD) as well as two enzymatically active domains (EADs) containing cysteine, histidine-dependent amidohydrolases/peptidases (CHAP, PF05257) and N-acetylmuramoyl-L-alanine amidase (amidase-3, PF01520) domains. The putative CBD of LysSA97 shows a specific binding spectrum against staphylococcal cells comparable to SH3 domain (PF08460) | Staphylococcus phage SA97 |
Synonyms | Comment | Organism |
---|---|---|
endolysin | - |
Staphylococcus phage SA97 |
LysSA97 | - |
Staphylococcus phage SA97 |
SA97_036 | - |
Staphylococcus phage SA97 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Staphylococcus phage SA97 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 65 | activity range, profile overview | Staphylococcus phage SA97 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Staphylococcus phage SA97 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | activity range, profile overview | Staphylococcus phage SA97 |
General Information | Comment | Organism |
---|---|---|
additional information | the putative cell wall binding domain (CBD) of LysSA97 shows a specific binding spectrum against staphylococcal cells comparable to SH3 domain (PF08460) | Staphylococcus phage SA97 |
physiological function | enzyme LysSA97 specifically lyses the staphylococcal strains and effectively disrupts staphylococcal biofilms | Staphylococcus phage SA97 |