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release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
-
-
-
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
catalytic mechanism, Asp160, Met161, Gly201, Arg202, and Phe219 are involved, active site structure, modeling of enzyme-substrate complex
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
catalytic mechanism, high preference towards large hydrophobic amino terminus residues, active site structure, Glu131 is involved in the catalytic mechanism, enzyme-substrate and enzyme-product complex modeling
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
hydrolyses peptide bonds formed by terminal hydrophobic amino acids such as leucine, methionine, and phenylalanine
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
M161 is involved in substrate binding at the active site cleft
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
preference for hydrophobic residues at the ultimate and the penultimate positions, D-amino acids at these positions reduce the activity, activity is not restricted by the length of substrate chains
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
catalytic pathway and reaction mechanism, catalytic residues are Glu131 and Tyr246, Tyr246 is involved in stabilization of the reaction transition state intermediate, also residue Glu151 is involved
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
raction mechanism, catalytic residues are Glu131 and Tyr246, residues Arg202 and Asp160 stabilize the reaction intermediate together with Glu131
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
structure and reaction mechanism
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
the enzyme prefers large hydrophobic aminoterminal residues, Glu131, Asp160, and Arg202 are involved in binding of the N-terminal substrate amino acid, substrate binding and reaction mechanism, tetrahedral reaction intermediate, modelling of the enzyme-substrate and enzyme-product complexes
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
catalytic reaction mechanism, a single proton transfer is involved in catalysis at pH 8.0, whereas two proton transfers are implicated at pH 6.5, involvement of a zinc-bound hydroxide as the reaction nucleophile, Tyr246 polarizes the carbonyl carbon and stabilizes the transition state, enzyme-substrate interaction, overview
-
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
structure and reaction mechanism
-
-
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4-nitrophenyl phenylphosphonate + H2O
4-nitrophenol + phenylphosphonate
-
-
-
-
?
4-nitrophenyl-L-leucine + H2O
4-nitrophenol + L-leucine
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
beta-Ala-OBzl + beta-Ala-OBzl
(beta-Ala)2-OBzl + benzyl alcohol
bis(4-nitrophenyl) phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
bis(4-nitrophenyl) phosphate + H2O
?
-
assay at pH 8.0, 30°C
-
-
?
bis(p-nitrophenyl)phosphate + H2O
?
-
-
-
?
D-Arg-OMe + L-Pro-OBzl
c(L-Pro-D-Arg) + benzyl alcohol + methanol
-
-
-
-
?
D-Arg-OMe + L-Pro-OBzl
L-Pro-D-Arg-OMe + benzyl alcohol
-
-
-
-
?
D-Leu-OBzl + D-Leu-OBzl
D-Leu-D-Leu-OBzl + benzyl alcohol
-
-
-
-
?
D-Leu-OMe + D-Leu-OMe
D-Leu-D-Leu-OMe + methanol
-
-
-
-
?
D-Leu-OMe + D-Pro-OBzl
D-Pro-D-Leu-OMe + benzyl alcohol
-
-
-
-
?
D-Leu-OMe + L-Pro-OBzl
L-Pro-D-Leu-OMe + benzyl alcohol
-
-
-
-
?
D-Phe-OMe + D-Pro-OBzl
D-Pro-D-Phe-OMe + benzyl alcohol
-
-
-
-
?
D-Phe-OMe + L-Pro-OBzl
L-Pro-D-Phe-OMe + benzyl alcohol
-
-
-
-
?
D-Trp-OMe + D-Pro-OBzl
D-Pro-D-Trp-OMe + benzyl alcohol
-
-
-
-
?
D-Trp-OMe + L-Pro-OBzl
L-Pro-D-Trp-OMe + benzyl alcohol
-
-
-
-
?
D-Tyr-OMe + D-Pro-OBzl
D-Pro-D-Tyr-OMe + benzyl alcohol
-
-
-
-
?
D-Tyr-OMe + L-Pro-OBzl
L-Pro-D-Tyr-OMe + benzyl alcohol
-
-
-
-
?
D-Val-OBzl + D-Val-OBzl
D-Val-D-Val-OBzl + benzyl alcohol
-
-
-
-
?
D-Val-OMe + L-Pro-OBzl
L-Pro-D-Val-OMe + benzyl alcohol
-
-
-
-
?
Glu-4-nitroanilide + H2O
Glu + 4-nitroaniline
-
5.4% of the activity with Lys-4-nitroanilide
-
-
?
Gly-4-nitroanilide + H2O
Gly + 4-nitroaniline
-
-
-
?
Gly-Leu-Gly + H2O
?
-
-
-
?
human hemoglobin alpha-chain + H2O
?
-
hydrolysis of the first few residues to proline at the 4th position
-
?
human hemoglobin beta-chain + H2O
?
-
hydrolysis of the first few residues to proline at the 5th position
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
L-Ala-OMe + L-Pro-OBzl
L-Pro-L-Ala-OMe + benzyl alcohol
-
-
-
-
?
L-alanine-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
L-alanine-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
L-Arg-OMe + beta-Ala-OBzl
beta-Ala-L-Arg-OMe + benzyl alcohol
L-Arg-OMe + L-Pro-OBzl
c(L-Pro-L-Arg) + benzyl alcohol + methanol
-
-
-
-
?
L-Arg-OMe + L-Pro-OBzl
L-Pro-L-Arg-OMe + benzyl alcohol
-
-
-
-
?
L-Asn-OMe + beta-Ala-OBzl
beta-Ala-L-Asn-OMe + benzyl alcohol
L-Asp-OMe + L-Pro-OBzl
L-Pro-L-Asp-OMe + benzyl alcohol
-
-
-
-
?
L-Glu-OMe + L-Pro-OBzl
L-Pro-L-Glu-OMe + benzyl alcohol
-
-
-
-
?
L-His-OMe + beta-Ala-OBzl
beta-Ala-L-His-OMe + benzyl alcohol
L-His-OMe + D-Pro-OBzl
c(D-Pro-L-His) + benzyl alcohol + methanol
-
-
-
-
?
L-His-OMe + D-Pro-OBzl
D-Pro-L-His-OMe + benzyl alcohol
-
-
-
-
?
L-His-OMe + L-Pro-OBzl
c(L-Pro-L-His) + benzyl alcohol + methanol
-
-
-
-
?
L-His-OMe + L-Pro-OBzl
L-Pro-L-His-OMe + benzyl alcohol
-
-
-
-
?
L-Ile-OMe + beta-Ala-OBzl
beta-Ala-L-Ile-OMe + benzyl alcohol
L-Ile-OMe + D-Pro-OBzl
D-Pro-L-Ile-OMe + benzyl alcohol
-
-
-
-
?
L-Ile-OMe + L-Pro-OBzl
L-Pro-L-Ile-OMe + benzyl alcohol
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-Leu-NH2 + H2O
L-Leu + NH3
L-Leu-O-methyl ester + H2O
L-Leu + methanol
L-Leu-OEt + beta-Ala-OBzl
beta-Ala-L-Leu-OEt + benzyl alcohol
-
-
-
-
?
L-Leu-OEt + beta-Ala-OBzl + beta-Ala-OBzl
beta-Ala-L-Leu-beta-Ala-OBzl + benzyl alcohol + ethanol
-
-
-
-
?
L-Leu-OEt + D-Pro-OBzl
D-Pro-L-Leu-OEt + benzyl alcohol
-
-
-
-
?
L-Leu-OEt + L-Pro-OBzl
L-Pro-L-Leu-OEt + benzyl alcohol
-
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
L-Lys-OMe + beta-Ala-OBzl
beta-Ala-L-Lys-OMe + benzyl alcohol
-
-
-
-
?
L-Lys-OMe + L-Pro-OBzl
L-Pro-L-Lys-OMe + benzyl alcohol
-
-
-
-
?
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
good substrate
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
L-Met-OMe + beta-Ala-OBzl
beta-Ala-L-Met-OMe + benzyl alcohol
-
-
-
-
?
L-Met-OMe + beta-Ala-OBzl + beta-Ala-OBzl
(beta-Ala)2-L-Met-OMe + benzyl alcohol
-
-
-
-
?
L-Met-OMe + beta-Ala-OBzl + beta-Ala-OBzl
beta-Ala-L-Met-beta-Ala-OBzl + benzyl alcohol + methanol
-
-
-
-
?
L-Met-OMe + D-Pro-OBzl
D-Pro-L-Met-OMe + benzyl alcohol
-
-
-
-
?
L-Met-OMe + L-Met-OMe + beta-Ala-OBzl
beta-Ala-L-Met-L-Met-OMe + benzyl alcohol + methanol
-
-
-
-
?
L-Met-OMe + L-Pro-OBzl
L-Pro-L-Met-OMe + benzyl alcohol
-
-
-
-
?
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
very good substrate
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
L-Phe-NH2 + H2O
L-Phe + NH3
-
-
-
?
L-Phe-O-methyl ester + H2O
L-Phe + methanol
-
-
-
?
L-Phe-OEt + beta-Ala-OBzl
beta-Ala-L-Phe-OEt + benzyl alcohol
-
-
-
-
?
L-Phe-OEt + beta-Ala-OBzl + beta-Ala-OBzl
beta-Ala-L-Phe-beta-Ala-OBzl + benzyl alcohol + ethanol
-
-
-
-
?
L-Phe-OEt + D-Pro-OBzl
D-Pro-L-Phe-OEt + benzyl alcohol
-
-
-
-
?
L-Phe-OEt + L-Phe-OEt + beta-Ala-OBzl
beta-Ala-L-Phe-L-Phe-OMe + benzyl alcohol + ethanol
-
-
-
-
?
L-Phe-OEt + L-Pro-OBzl
L-Pro-L-Phe-OEt + benzyl alcohol
-
-
-
-
?
L-phenylalanine ethyl ester + L-phenylalanine + H2O
L-Phe-L-Phe + cyclo(L-phenylalanine-L-phenylalanine) + L-Phe-L-Phe ethyl ester
L-phenylalanine-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
-
very good substrate
-
?
L-Pro-4-nitroanilide + H2O
L-Pro + 4-nitroaniline
very low activity
-
-
?
L-Pro-OMe + beta-Ala-OBzl
L-Pro-beta-Ala-OBzl + methanol
-
-
-
-
?
L-Pro-OMe + beta-Ala-OBzl + beta-Ala-OBzl
L-Pro-(beta-Ala)2-OBzl + methanol + benzyl alcohol
-
-
-
-
?
L-proline-4-nitroanilide + H2O
L-proline + 4-nitroaniline
-
good substrate
-
?
L-proline-p-nitroanilide + H2O
L-Pro + 4-nitroaniline
L-Ser-OMe + L-Pro-OBzl
L-Pro-L-Ser-OMe + benzyl alcohol
-
-
-
-
?
L-Thr-OMe + beta-Ala-OBzl
beta-Ala-L-Thr-OMe + benzyl alcohol
-
-
-
-
?
L-Thr-OMe + L-Pro-OBzl
L-Pro-L-Thr-OMe + benzyl alcohol
-
-
-
-
?
L-Thr-OMe + L-Thr-OMe
(L-Thr)2-OMe + methanol
-
-
-
-
?
L-Thr-OMe + L-Thr-OMe
L-Thr-L-Thr-OMe + methanol
-
-
-
-
?
L-Trp-OMe + beta-Ala-OBzl
beta-Ala-L-Trp-OMe + benzyl alcohol
-
-
-
-
?
L-Trp-OMe + D-Pro-OBzl
D-Pro-L-Trp-OMe + benzyl alcohol
-
-
-
-
?
L-Trp-OMe + L-Pro-OBzl
L-Pro-L-Trp-OMe + benzyl alcohol
-
-
-
-
?
L-Tyr-OMe + beta-Ala-OBzl
beta-Ala-L-Tyr-OMe + benzyl alcohol
-
-
-
-
?
L-Tyr-OMe + beta-Ala-OBzl + beta-Ala-OBzl
(beta-Ala)2-L-Tyr-OMe + benzyl alcohol
-
-
-
-
?
L-Tyr-OMe + D-Pro-OBzl
D-Pro-L-Tyr-OMe + benzyl alcohol
-
-
-
-
?
L-Tyr-OMe + L-Pro-OBzl
L-Pro-L-Tyr-OMe + benzyl alcohol
-
-
-
-
?
L-Tyr-OMe + L-Tyr-OMe + beta-Ala-OBzl
beta-Ala-L-Tyr-L-Tyr-OMe + benzyl alcohol + methanol
-
-
-
-
?
L-Val-OBzl + L-Val-OBzl
L-Val-L-Val-OBzl + benzyl alcohol
-
-
-
-
?
L-Val-OMe + beta-Ala-OBzl
beta-Ala-L-Val-OMe + benzyl alcohol
-
-
-
-
?
L-Val-OMe + beta-Ala-OBzl + beta-Ala-OBzl
(beta-Ala)2-L-Val-OMe + benzyl alcohol
-
-
-
-
?
L-Val-OMe + D-Pro-OBzl
D-Pro-L-Val-OMe + benzyl alcohol
-
-
-
-
?
L-Val-OMe + L-Pro-OBzl
L-Pro-L-Val-OMe + benzyl alcohol
-
-
-
-
?
L-valine-4-nitroanilide + H2O
L-valine + 4-nitroaniline
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-Gly-Gly + H2O
Leu + Gly-Gly
-
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Pro-4-nitroanilide + H2O
Pro + 4-nitroaniline
-
11.7% of the activity with Lys-4-nitroanilide
-
-
?
Val-4-nitroanilide + H2O
Val + 4-nitroaniline
-
-
-
?
additional information
?
-
4-nitrophenyl-L-leucine + H2O
4-nitrophenol + L-leucine
-
-
-
-
?
4-nitrophenyl-L-leucine + H2O
4-nitrophenol + L-leucine
-
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
-
105.2% of the activity with Lys-4-nitroanilide
-
-
?
beta-Ala-OBzl + beta-Ala-OBzl
(beta-Ala)2-OBzl + benzyl alcohol
-
-
-
-
?
beta-Ala-OBzl + beta-Ala-OBzl
(beta-Ala)2-OBzl + benzyl alcohol
-
-
-
-
?
Hemoglobin + H2O
?
-
human hemoglobin
-
-
?
Hemoglobin + H2O
?
-
human hemoglobin
-
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
-
-
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
very low activity
-
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
very low activity
-
-
?
L-alanine-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
-
-
-
?
L-alanine-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
-
-
-
?
L-alanine-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
-
?
L-alanine-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
good substrate
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
low activity
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
low activity
-
-
?
L-Arg-OMe + beta-Ala-OBzl
beta-Ala-L-Arg-OMe + benzyl alcohol
-
-
-
-
?
L-Arg-OMe + beta-Ala-OBzl
beta-Ala-L-Arg-OMe + benzyl alcohol
-
-
-
-
?
L-Asn-OMe + beta-Ala-OBzl
beta-Ala-L-Asn-OMe + benzyl alcohol
-
-
-
-
?
L-Asn-OMe + beta-Ala-OBzl
beta-Ala-L-Asn-OMe + benzyl alcohol
-
-
-
-
?
L-His-OMe + beta-Ala-OBzl
beta-Ala-L-His-OMe + benzyl alcohol
-
-
-
-
?
L-His-OMe + beta-Ala-OBzl
beta-Ala-L-His-OMe + benzyl alcohol
-
-
-
-
?
L-Ile-OMe + beta-Ala-OBzl
beta-Ala-L-Ile-OMe + benzyl alcohol
-
-
-
-
?
L-Ile-OMe + beta-Ala-OBzl
beta-Ala-L-Ile-OMe + benzyl alcohol
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
best substrate
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
best substrate
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
preferred substrate of the wild-type enzyme
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
best substrate
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
preferred substrate of the wild-type enzyme
-
-
?
L-Leu-NH2 + H2O
L-Leu + NH3
-
-
-
?
L-Leu-NH2 + H2O
L-Leu + NH3
-
-
-
?
L-Leu-O-methyl ester + H2O
L-Leu + methanol
-
-
-
?
L-Leu-O-methyl ester + H2O
L-Leu + methanol
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
best of the amino acid 4-nitroanilide substrates
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
best of the amino acid 4-nitroanilide substrates
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
-
low activity
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
low activity
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
low activity
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
low activity
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
low activity
-
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
low activity
-
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
-
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
low activity
-
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
-
-
?
L-phenylalanine ethyl ester + L-phenylalanine + H2O
L-Phe-L-Phe + cyclo(L-phenylalanine-L-phenylalanine) + L-Phe-L-Phe ethyl ester
-
assay at 50°C
-
-
?
L-phenylalanine ethyl ester + L-phenylalanine + H2O
L-Phe-L-Phe + cyclo(L-phenylalanine-L-phenylalanine) + L-Phe-L-Phe ethyl ester
-
assay at 50°C
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
-
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
-
-
-
?
L-proline-p-nitroanilide + H2O
L-Pro + 4-nitroaniline
-
-
-
-
?
L-proline-p-nitroanilide + H2O
L-Pro + 4-nitroaniline
-
-
-
-
?
L-valine-4-nitroanilide + H2O
L-valine + 4-nitroaniline
-
-
-
?
L-valine-4-nitroanilide + H2O
L-valine + 4-nitroaniline
-
good substrate
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
12.2% of the activity with Lys-4-nitroanilide
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
assay at pH 8.0, 30°C
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
?
Met-enkephalin + H2O
?
-
substrate is a pentapeptide with sequence Tyr-Gly-Gly-Phe-Met, stepwise degradation from the N-terminus
-
-
?
Met-enkephalin + H2O
?
-
substrate is a pentapeptide with sequence Tyr-Gly-Gly-Phe-Met, stepwise degradation from the N-terminus
-
-
?
peptide + H2O
?
-
-
?
peptide + H2O
?
-
substrate specificity
-
?
peptide + H2O
?
-
high preference towards large hydrophobic amino terminus residues
-
?
additional information
?
-
-
no activity with Gly-Pro-4-nitroanilide and Ala-Pro-4-nitroanilide, no acitivity with Xaa-Pro N-terminal sequences, glycine-4-nitroanilide and acidic amino acid 4-nitroanilide are very poor substrates
-
?
additional information
?
-
-
substrates with blocked amino groups are partially hydrolyzed, poor activity with Val-Gly, Val-Leu, and Trp-Leu, Gly-Gly-Gly and D-leucine-D-leucine are no substrates
-
?
additional information
?
-
-
aminopeptidases are involved in peptides processing and degradation, and are important in uptake of nutrients, regulation, overview
-
-
?
additional information
?
-
-
no activity with L-Glu-4-nitroanilide, L-Arg-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide, L-Ala-4-nitroanilide and L-Pro-4-nitroanilide are poor substrates
-
-
?
additional information
?
-
-
the enzyme is active on a wide variety of peptides substrates, no activity with D-Leu-D-Leu, no prolidase activity, but release of N-terminal prolyl residues
-
-
?
additional information
?
-
-
the enzyme shows broad substrate specificity preferring N-terminal Leu or Met and Phe, but is not able to hydrolyse peptide substrates bonds with formed by acidic amino acids in the P1 position or proline in the P1 or P1' position
-
-
?
additional information
?
-
the enzyme is active toward various peptides with different N-terminal side chains and specific toward hydrophobic ones, the enzyme also exhibits a significant activity toward the hydrolysis of the phosphodiester bis(p-nitrophenyl)phosphate, overview, active site structure involves the three auxiliary amino acid side chains of Tyr246, Glu131, and Arg202 that are involved in catalysis, modeling of substrate binding using the crystal structure of the enzyme, overview, the activity shows proton inventory and viscosity dependence, overview
-
-
?
additional information
?
-
-
the enzyme is active toward various peptides with different N-terminal side chains and specific toward hydrophobic ones, the enzyme also exhibits a significant activity toward the hydrolysis of the phosphodiester bis(p-nitrophenyl)phosphate, overview, active site structure involves the three auxiliary amino acid side chains of Tyr246, Glu131, and Arg202 that are involved in catalysis, modeling of substrate binding using the crystal structure of the enzyme, overview, the activity shows proton inventory and viscosity dependence, overview
3.4.11.24 aminopeptidase S
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