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Literature summary for 3.4.11.24 extracted from
- Purification and characterization of recombinant Streptomyces griseus aminopeptidase (2003), Protein Expr. Purif., 30, 62-68.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Streptomyces lividans by insertional cloning and protoplast transformation, the expression system contains the constitutive Streptomyces fradiae aph promoter | Streptomyces griseus |
| gene SGAP, standard protoplast transformation and expression in Streptomyces lividans, excretion to the medium | Streptomyces griseus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces griseus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-metallo-exoprotease | Streptomyces griseus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26800 | - |
recombinant enzyme, gel filtration | Streptomyces griseus |
| 30800 | - |
1 * 30800, recombinant enzyme, SDS-PAGE | Streptomyces griseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptide + H2O | Streptomyces griseus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Streptomyces lividans cells by diafiltration, hydrophobic interaction chromatography, and gel filtration to homogeneity | Streptomyces griseus |
| recombinant from Streptomyces lividans cell culture medium, high purity, 19% recovery | Streptomyces griseus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 625.1 | - |
purified enzyme | Streptomyces griseus |
| 625.1 | - |
purified recombinant enzyme | Streptomyces griseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, liquid and freeze-dried, stable for at least 8 weeks, no loss of activity | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Streptomyces griseus | ? | - |
? | |
| L-leucine-4-nitroanilide + H2O | - |
Streptomyces griseus | L-leucine + 4-nitroaniline | - |
? | |
| peptide + H2O | - |
Streptomyces griseus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30800, recombinant enzyme, SDS-PAGE | Streptomyces griseus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M28.003 | Merops-ID | Streptomyces griseus |
| SGAP | - |
Streptomyces griseus |
| Streptomyces griseus aminopeptidase | - |
Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Streptomyces griseus |
| 37 | - |
assay at | Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 69 | - |
recombinant enzyme, stable at for at least 1 h | Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Streptomyces griseus |
| 8 | - |
assay at | Streptomyces griseus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces griseus | isoelectric focusing | - |
5.2 |
| Streptomyces griseus | recombinant enzyme, isoelectric focusing and amino acid sequence calculation | - |
5.2 |
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