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Literature summary for 3.4.11.24 extracted from

  • Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Alteration of leucine aminopeptidase from Streptomyces septatus TH-2 to phenylalanine aminopeptidase by site-directed mutagenesis (2005), Appl. Environ. Microbiol., 71, 7229-7235.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.24

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces septatus

Protein Variants

Protein Variants Comment Organism
F221A site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme Streptomyces septatus
F221G site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme Streptomyces septatus
F221S site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme Streptomyces septatus
additional information alteration of leucine aminopeptidase to phenylalanine aminopeptidase by site-directed mutagenesis Streptomyces septatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of recombinant wild-type and F221 mutant enzymes Streptomyces septatus
0.25
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
0.34
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
0.36
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
0.44
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
0.68
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
1.85
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
6.9
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
6.98
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
7.68
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
8.31
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
12.8
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
15.6
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
15.8
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
16.1
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
24.2
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
42.7
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus

Organism

Organism UniProt Comment Textmining
Streptomyces septatus Q75V72 gene Ssap
-
Streptomyces septatus TH-2 Q75V72 gene Ssap
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Streptomyces septatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces septatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O preferred substrate of the wild-type enzyme Streptomyces septatus L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O preferred substrate of the wild-type enzyme Streptomyces septatus TH-2 L-Leu + 4-nitroaniline
-
 ?
L-Leu-NH2 + H2O
-
 Streptomyces septatus L-Leu + NH3
-
 ?
L-Leu-NH2 + H2O
-
 Streptomyces septatus TH-2 L-Leu + NH3
-
 ?
L-Leu-O-methyl ester + H2O
-
 Streptomyces septatus L-Leu + methanol
-
 ?
L-Leu-O-methyl ester + H2O
-
 Streptomyces septatus TH-2 L-Leu + methanol
-
 ?
L-Phe-4-nitroanilide + H2O
-
 Streptomyces septatus L-Phe + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O
-
 Streptomyces septatus TH-2 L-Phe + 4-nitroaniline
-
 ?
L-Phe-NH2 + H2O
-
 Streptomyces septatus L-Phe + NH3
-
 ?
L-Phe-O-methyl ester + H2O
-
 Streptomyces septatus L-Phe + methanol
-
 ?
additional information substrate specificities of recombinant wild-type and F221 mutant enzymes, overview Streptomyces septatus ?
-
 ?
additional information substrate specificities of recombinant wild-type and F221 mutant enzymes, overview Streptomyces septatus TH-2 ?
-
 ?

Synonyms

Synonyms Comment Organism
leucine aminopeptidase
-
 Streptomyces septatus
SSAP
-
 Streptomyces septatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces septatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.64
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
10.7
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
17.6
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
19.3
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
31.7
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
34
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
43.8
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
54.3
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
64.2
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
65.9
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
104
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
108
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
115
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
142
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
155
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
169
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
224
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
229
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces septatus