Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme is useful in many biotechnological applications e.g. in processing of recombinant DNA, proteins, and fusion protein products | Streptomyces griseus |
Crystallization (Comment) | Organism |
---|---|
purified enzyme complexed with methionine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 0.1 M methionine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml reservoir of the precipitant solution, 3-4 days, X-ray diffraction structure determination at 1.53 A high resolution and analysis | Streptomyces griseus |
purified native enzyme in complex with product analogous weak inhibiting amino acids phenylalanine, leucine, and methionine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM L-methionine or 200 mM L-leucine, the precipitant solution contains 24% w/v PEG 4000 and 0.1 M ammonium sulfate, equilibration against 1 ml reservoir solution, microseeding, 3-4 days, with phenylalanine a protein solution containing 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM Phe is mixed with a reservoir solution containing 18% w/v PEG 4000, and 0.1 M acetate buffer, pH 5.5, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.8 A, 1.7 A, and 1.53 A resolution, respectively, structure modelling | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leucine | weak inhibitor, binding structure, overview | Streptomyces griseus | |
methionine | weak inhibitor, binding structure, overview | Streptomyces griseus | |
phenylalanine | weak inhibitor, binding structure, overview | Streptomyces griseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces griseus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | modulating the enzyme activity | Streptomyces griseus | |
Zn2+ | 2 mol zinc per mol of enzyme, binding structure | Streptomyces griseus | |
Zn2+ | the enzyme is a double-zinc exopeptidase, binding structure, overview | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000 | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Streptomyces griseus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Streptomyces griseus | P80561 | purified enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues | catalytic mechanism, Asp160, Met161, Gly201, Arg202, and Phe219 are involved, active site structure, modeling of enzyme-substrate complex | Streptomyces griseus | |
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues | the enzyme prefers large hydrophobic aminoterminal residues, Glu131, Asp160, and Arg202 are involved in binding of the N-terminal substrate amino acid, substrate binding and reaction mechanism, tetrahedral reaction intermediate, modelling of the enzyme-substrate and enzyme-product complexes | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | - |
Streptomyces griseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000 | Streptomyces griseus |
Synonyms | Comment | Organism |
---|---|---|
M28.003 | Merops-ID | Streptomyces griseus |
SGAP | - |
Streptomyces griseus |
Streptomyces griseus aminopeptidase | - |
Streptomyces griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is heat-stable | Streptomyces griseus |