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Literature summary for 3.4.11.24 extracted from

  • Gilboa, R.; Greenblatt, H.M.; Perach, M.; Spungin-Bialik, A.; Lessel, U.; Wohlfahrt, G.; Schomburg, D.; Blumberg, S.; Shoham, G.
    Interactions of Streptomyces griseus aminopeptidase with a methionine product analogue: a structural study at 1.53 A resolution (2000), Acta Crystallogr. Sect. D, D56, 551-558.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the enzyme is useful in many biotechnological applications e.g. in processing of recombinant DNA, proteins, and fusion protein products Streptomyces griseus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme complexed with methionine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 0.1 M methionine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml reservoir of the precipitant solution, 3-4 days, X-ray diffraction structure determination at 1.53 A high resolution and analysis Streptomyces griseus
purified native enzyme in complex with product analogous weak inhibiting amino acids phenylalanine, leucine, and methionine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM L-methionine or 200 mM L-leucine, the precipitant solution contains 24% w/v PEG 4000 and 0.1 M ammonium sulfate, equilibration against 1 ml reservoir solution, microseeding, 3-4 days, with phenylalanine a protein solution containing 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM Phe is mixed with a reservoir solution containing 18% w/v PEG 4000, and 0.1 M acetate buffer, pH 5.5, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.8 A, 1.7 A, and 1.53 A resolution, respectively, structure modelling Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
leucine weak inhibitor, binding structure, overview Streptomyces griseus
methionine weak inhibitor, binding structure, overview Streptomyces griseus
phenylalanine weak inhibitor, binding structure, overview Streptomyces griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces griseus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ modulating the enzyme activity Streptomyces griseus
Zn2+ 2 mol zinc per mol of enzyme, binding structure Streptomyces griseus
Zn2+ the enzyme is a double-zinc exopeptidase, binding structure, overview Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
1 * 30000 Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide + H2O Streptomyces griseus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-
Streptomyces griseus P80561 purified enzyme
-

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues catalytic mechanism, Asp160, Met161, Gly201, Arg202, and Phe219 are involved, active site structure, modeling of enzyme-substrate complex Streptomyces griseus
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues the enzyme prefers large hydrophobic aminoterminal residues, Glu131, Asp160, and Arg202 are involved in binding of the N-terminal substrate amino acid, substrate binding and reaction mechanism, tetrahedral reaction intermediate, modelling of the enzyme-substrate and enzyme-product complexes Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptide + H2O
-
Streptomyces griseus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000 Streptomyces griseus

Synonyms

Synonyms Comment Organism
M28.003 Merops-ID Streptomyces griseus
SGAP
-
Streptomyces griseus
Streptomyces griseus aminopeptidase
-
Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme is heat-stable Streptomyces griseus