Crystallization (Comment) | Organism |
---|---|
protein with or without bound Zn2+ or replaced with Hg2+, hanging drop vapour diffusion method, 20 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 4-5 weeks to full size crystals, X-ray diffraction structure determination at 2.1 to 1.75 A resolution and analysis | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | location of binding site is in about 25 A distance from the zinc binding site, determination of structural environment | Streptomyces griseus | |
Zn2+ | 2 mol zinc per mol of enzyme, tightly bound at the active site in a distance of 3.6 A of each other, determination of structural environment | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Streptomyces griseus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
purified enzyme from commercial product | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues | M161 is involved in substrate binding at the active site cleft | Streptomyces griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial product | protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus | Streptomyces griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | - |
Streptomyces griseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M28.003 | Merops-ID | Streptomyces griseus |
SGAP | - |
Streptomyces griseus |