Application | Comment | Organism |
---|---|---|
analysis | the enzyme is clinically important as a model for undestanding the structure and mechanism of action of other metallopeptidases | Streptomyces griseus |
Cloned (Comment) | Organism |
---|---|
gene Sgap, screening of 21 strains with strain NBRC12875 showing the highest activity | Streptomyces griseus |
Protein Variants | Comment | Organism |
---|---|---|
D3A/D262G | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Streptomyces griseus |
E196A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces griseus |
additional information | construction of chimeric enzymes from the enzymes of Streptomyces griseus and Streptomyces septatus by DNA in vivo shuffling and site-directed mutagenesis for calcium activation and binding studies, overview | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-histidine | - |
Streptomyces griseus | |
L-serine | - |
Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.54 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant D3A/D262G, in absence of Ca2+ | Streptomyces griseus | |
2.61 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme, in absence of Ca2+ | Streptomyces griseus | |
3.47 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant E196A, in presence of Ca2+ | Streptomyces griseus | |
3.79 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant E196A, in absence of Ca2+ | Streptomyces griseus | |
3.91 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant chimeric mutant, in presence of Ca2+ | Streptomyces griseus | |
4.02 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant chimeric mutant, in absence of Ca2+ | Streptomyces griseus | |
4.02 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme, in presence of Ca2+ | Streptomyces griseus | |
4.07 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant D3A/D262G, in presence of Ca2+ | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates and stabilizes, influences substrate specificity, Asp173 and Asp174 are key residues in Ca2+ binding and important for enzyme activity, residues Asp3, Ile4, Asp262, and Asp266 are also involved in calcium binding but are important for enzyme stabilization, overview, binding capacity of recombinant wild-type and mutant enzymes, overview | Streptomyces griseus | |
Zn2+ | the enzyme is a double-zinc aminopeptidase, metal ions are bound at the active site | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | Q5WA30 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant wild-type and mutant enzymes, overview | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Streptomyces griseus | L-leucine + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Streptomyces griseus |
SGAP | - |
Streptomyces griseus |
Streptomyces griseus leucine aminopeptidase | - |
Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant E196A, in presence of Ca2+ | Streptomyces griseus | |
0.55 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant E196A, in absence of Ca2+ | Streptomyces griseus | |
0.97 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme, in presence of Ca2+ | Streptomyces griseus | |
1.08 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant D3A/D262G, in presence of Ca2+ | Streptomyces griseus | |
2.12 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme, in absence of Ca2+ | Streptomyces griseus | |
2.71 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant D3A/D262G, in absence of Ca2+ | Streptomyces griseus | |
3 | 6 | L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant D3A/D262G, in absence of Ca2+ | Streptomyces griseus | |
4.72 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant chimeric mutant, in presence of Ca2+ | Streptomyces griseus | |
5.2 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant chimeric mutant, in absence of Ca2+ | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces griseus |