EC Number   |
|---|
   3.4.11.24 | DNA and amino acid sequence determination and analysis |
| 3.4.11.24 | DNA and amino acid sequence determination, subcloning and expression of the recombinant enzyme possessing a Asp70 and Asp184 residues in Escherichia coli strain BL21(DE3), the soluble enzyme is inducible by growth on 1 M sorbitol, optimization of the expression method |
| 3.4.11.24 | expression in Escherichia coli BL21 |
| 3.4.11.24 | expression in Streptomyces lividans by insertional cloning and protoplast transformation, the expression system contains the constitutive Streptomyces fradiae aph promoter |
| 3.4.11.24 | expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 3.4.11.24 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 3.4.11.24 | gene Sgap, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli |
| 3.4.11.24 | gene Sgap, screening of 21 strains with strain NBRC12875 showing the highest activity |
| 3.4.11.24 | gene SGAP, standard protoplast transformation and expression in Streptomyces lividans, excretion to the medium |
| 3.4.11.24 | gene Ssap, genomic library screening, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli strain BL21(DE3) |
