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Literature summary for 3.4.11.24 extracted from

  • Vosbeck, K.D.; Greenberg, B.D.; Awad, W.M., Jr.
    The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). Specificity and immobilization of aminopeptidase (1975), J. Biol. Chem., 250, 3981-3987.
    View publication on PubMed
Search for more literature for 3.4.11.24

Inhibitors

Inhibitors Comment Organism Structure
diisopropylphosphofluoridate pretreatment, complete inhibition Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 L-leucine-4-nitroanilide pH 8.0, 22°C, native aminopeptidases 1 and 2 Streptomyces griseus
0.72
-
 L-leucine-4-nitroanilide pH 8.0, 22°C, acetylated aminopeptidases 1 and 2 Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
 aminopeptidase 1 and 2, i.e. AP-1 and AP-2
-

Purification (Commentary)

Purification (Comment) Organism
from commercial product, purification of the acetylated enzyme derivatives by DEAE cellulose chromatography Streptomyces griseus

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues preference for hydrophobic residues at the ultimate and the penultimate positions, D-amino acids at these positions reduce the activity, activity is not restricted by the length of substrate chains Streptomyces griseus
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation pronase preparation, containing no prolidase acivity Streptomyces griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Leu-Gly + H2O
-
 Streptomyces griseus ?
-
 ?
human hemoglobin alpha-chain + H2O hydrolysis of the first few residues to proline at the 4th position Streptomyces griseus ?
-
 ?
human hemoglobin beta-chain + H2O hydrolysis of the first few residues to proline at the 5th position Streptomyces griseus ?
-
 ?
L-leucine-4-nitroanilide + H2O
-
 Streptomyces griseus L-leucine + 4-nitroaniline
-
 ?
Leu-Gly-Gly + H2O
-
 Streptomyces griseus Leu + Gly-Gly
-
 ?
additional information substrates with blocked amino groups are partially hydrolyzed, poor activity with Val-Gly, Val-Leu, and Trp-Leu, Gly-Gly-Gly and D-leucine-D-leucine are no substrates Streptomyces griseus ?
-
 ?
peptide + H2O
-
 Streptomyces griseus ?
-
 ?
peptide + H2O substrate specificity Streptomyces griseus ?
-
 ?

Synonyms

Synonyms Comment Organism
AP
-
 Streptomyces griseus
M28.003 Merops-ID Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
75
-
 L-leucine-4-nitroanilide pH 8.0, 22°C, acetylated aminopeptidases 2 Streptomyces griseus
111
-
 L-leucine-4-nitroanilide pH 8.0, 22°C, native aminopeptidases 2 Streptomyces griseus
116
-
 L-leucine-4-nitroanilide pH 8.0, 22°C, acetylated aminopeptidases 1 Streptomyces griseus
153
-
 L-leucine-4-nitroanilide pH 8.0, 22°C, native aminopeptidases 1 Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces griseus