Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, the reservoir solution contains 0.1 M HEPES/NaOH, pH 7.6, 2.0 M ammonium sulfate, and 2% PEG 400, 4 weeks, X-ray diffraction structure determination and analysis at 1.8 A resolution | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is completely abolished by metal chelating agents, but can be restored by addition of zinc or cobalt | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme contains two metal ions with high occupancy and a third metal ion with low occupancy at the active site of the enzyme molecule, Glu319 and a water molecule are bridging, Glu253, His348, His381, Tyr355, and Asp383 are involved in metal binding, structure analysis | Staphylococcus aureus | |
additional information | determination of metal ion identity | Staphylococcus aureus | |
Zn2+ | the enzyme contains two metal ions with high occupancy and a third metal ion with low occupancy at the active site of the enzyme molecule, Glu319 and a water molecule are bridging, Glu253, His348, His381, Tyr355, and Asp383 are involved in metal binding, structure analysis | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus NCTC8325-4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-enkephalin + H2O | substrate is a pentapeptide with sequence Tyr-Gly-Gly-Phe-Met, stepwise degradation from the N-terminus | Staphylococcus aureus | ? | - |
? | |
Met-enkephalin + H2O | substrate is a pentapeptide with sequence Tyr-Gly-Gly-Phe-Met, stepwise degradation from the N-terminus | Staphylococcus aureus NCTC8325-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the enzyme forms an inactive dimer in the crystal with a large internal cavity with the active sites located at the opposing ends of the cavity essentially inaccessible from the outside | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase S | - |
Staphylococcus aureus |
AmpS | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |