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Information on EC 3.2.1.31 - beta-glucuronidase
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3.2.1.31 beta-glucuronidaseSpecify your search results
Word Map
- 3.2.1.31
- lysosomal
- urine
- tobacco
-
histochemical
- urinary
- neutrophil
- bile
- leukocyte
- lysozyme
-
polymorphonuclear
- granule
- hydrolases
- cathepsins
- agrobacterium
- mucopolysaccharidosis
-
protoplasts
-
degranulation
-
glucuronic
- glycosaminoglycans
-
cytochemical
-
sulfatase
- cauliflower
- tumefaciens
-
fecal
- biliary
-
n-acetyl-beta-d-glucosaminidase
- beta-n-acetylglucosaminidase
- alpha-mannosidase
- alpha-galactosidase
-
deconjugation
- hygromycin
- arylsulphatase
-
calli
-
bombard
- nitroreductase
- beta-d-galactosidase
- beta-hexosaminidase
-
azurophilic
- zymosan
-
agrobacterium-mediated
- fmlp
- glycosidases
- neomycin
- diagnostics
-
alpha-naphthyl
- analysis
- alpha-l-fucosidase
- pomatia
- alpha-l-iduronidase
- hexosaminidase
- molecular biology
- medicine
- toxicology
- biotechnology
- synthesis
- nutrition
- drug development
-
coliforms
-
crevicular
The enzyme appears in viruses and cellular organisms
Synonyms
beta-D-glucuronidase, Beta-D-glucuronoside glucuronosohydrolase, Beta-G1, beta-Gluc, beta-glucuronidase, beta-glucuronidase A, betaG, BLG, exo-beta-D-glucuronidase, exo-beta-glucuronidase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Beta-D-glucuronoside glucuronosohydrolase
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-
-
-
Beta-G1
-
-
-
-
beta-glucuronidase
betaG
exo-beta-D-glucuronidase
-
-
-
-
GlcAase
glucuronidase, beta-glucuronide glucuronohydrolase
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-
-
-
GUS
GusA
GusB
ketodase
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-
-
-
PGUS
TM1062
additional information
beta-glucuronidase
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fusion product with stachyose synthetase, EC 2.4.1.67
GUS
-
-
-
-
additional information
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the enzyme belongs to glycosyl hydrolase family 2 with three conserved domains
additional information
-
the enzyme belongs to glycosyl hydrolase family 2 with three conserved domains
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additional information
the enzyme belongs to the glycosyl hydrolases family 2
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol
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-
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a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol
reaction mechanism, active site with catalytic nucleophile E576, residue E383 is also involved in catalysis
a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol
reaction mechanism, active site with catalytic nucleophile E576, residue E383 is also involved in catalysis
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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exoglycosidic
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
beta-D-glucuronoside glucuronosohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9001-45-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1S)-1-(chloromethyl)-3-([5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]carbonyl)-2,3-dihydro-1H-benzo[e]indol-5-yl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + [(1S)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indol-3-yl][5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]methanone
-
-
-
-
?
(1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-{[({2-[({[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy}carbonyl)amino]-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl}oxy)carbonyl]amino}-alpha-D-lyxo-hexopyranoside + H2O
D-glucuronate + (1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-[({[2-({[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl}amino)-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl]oxy}carbonyl)amino]-alpha-D-lyxo-hexopyranoside
-
-
-
-
?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2 + H2O
D-glucuronate + ?
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no activity with enzyme acting on chondroitin, 142% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3 + H2O
D-glucuronate + ?
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no activity with enzyme acting on chondroitin, 63% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2 + H2O
D-glucuronate + ?
-
no activity with enzyme acting on chondroitin, 50% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3 + H2O
D-glucuronate + ?
-
no activity with enzyme acting on chondroitin, 33% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAcbeta1-)2 + H2O
D-glucuronate + ?
-
66% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 90% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAcbeta1-)3 + H2O
D-glucuronate + ?
-
56% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 71% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GlcNAcbeta1-)3 + H2O
D-glucuronate + ?
-
81% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 73% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranoside + H2O
6-mercaptopurine + D-glucopyranose
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-
-
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1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosiduronamide + H2O
6-mercaptopurine + 1-deoxy-D-glucopyranosiduronamide
-
-
-
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1-O-([4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamoyl)-beta-D-glucuronate + H2O
D-glucuronate + [4-[(10R,11S)-10-[[((2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamic acid
-
-
-
-
?
1-O-[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamic acid
-
-
-
-
?
1-O-[(4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamic acid
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-
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?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4,7-dimethyl-3,8,13-trioxo-11,13-diphenyl-2,9-dioxa-4,7,12-triazatridec-1-yl]phenyl beta-D-glucopyranosiduronate + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4,7-dimethyl-3,8-dioxo-2,9-dioxa-4,7-diazaundecan-11-oate
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-
-
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?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
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-
-
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?
4-([[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamate
-
-
-
-
?
4-([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-9-yl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (4S)-4-ethyl-4-hydroxy-9-[(4-hydroxy-3-nitrobenzyl)oxy]-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione
-
-
-
-
?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O
?
-
rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide
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-
?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O
?
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rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide
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-
?
4-Me-beta-GlcA-(1-6)-Gal + H2O
?
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rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + beta-D-glucuronic acid
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronic acid + H2O
4-methylumbelliferone + beta-D-glucuronic acid
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferol + D-glucuronate
-
-
-
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?
4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
preferred substrate
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-
?
4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]-2-nitrophenyl beta-D-glucuronate + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-10-[(S)-(benzoylamino)(phenyl)methyl]-1-(4-hydroxy-3-nitrophenyl)-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
-
-
-
-
?
4-[([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)methyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-carboxylate
-
-
-
-
?
4-[([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamoyl]oxy)methyl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxybenzyl [(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamate
-
-
-
-
?
4-[2-[1-(2-[2-[2-(beta-D-glucopyranuronosyloxy)ethoxy]ethoxy]ethyl)-1H-1,2,3-triazol-4-yl]-1-([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)ethyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 2-[2-(2-[4-[2-([[(3beta,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)-2-(4-hydroxy-3-nitrophenyl)ethyl]-1H-1,2,3-triazol-1-yl]ethoxy)ethoxy]ethyl beta-D-glucopyranosiduronic acid
-
-
-
-
?
5-bromo-4-chloro-3-indoyl-beta-D-glucuronide + H2O
5-bromo-4-chloro-1H-indol-3-ol + beta-D-glucuronate
-
-
-
-
?
ammonium 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosidurate + H2O
6-mercaptopurine + ammonium 1-deoxy-(6-thiopurinyl)-beta-D-glucopyranosiduronate
-
-
-
-
-
apigenin 7,4'-diglucuronide + H2O
apigenin 7-O-glucuronide + glucuronic acid
-
-
-
-
-
beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
-
67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
beta-GlcA(1-6)betaGal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
-
-
94.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
beta-GlcA-(1-3)-Gal + H2O
?
-
rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O
beta-Gal-(1-6)-Gal + beta-D-glucuronic acid
-
rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
beta-GlcA-(1-6)-Gal + H2O
D-galactose + beta-D-glucuronic acid
-
rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
carboxyumbelliferyl-beta-D-glucuronide + H2O
carboxyumbelliferone + beta-D-glucuronate
-
-
-
-
?
chondroitin + H2O
D-glucuronate + ?
-
MW 3500 Da: 23% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 41% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 8000 Da: 11% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 28% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 15000 Da: 6% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 9% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
curcumin beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
-
-
-
?
curcumin-beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
curcumin, despite low systemic bioavailability, may be enzymatically activated (deconjugated) within GUSB-enriched bone to exert protective effects, a metabolic process that can also contribute to bone-protective effects of other highly glucuronidated dietary polyphenols
-
-
?
GlcA-beta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
-
?
GlcAbeta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
-
85.7% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
-
?
GlcAbeta1-3GalNAc(4-OSO3-) + H2O
D-glucuronate + N-acetylamino-beta-D-galactopyranose 4-sulfate
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no activity with enzyme acting on chondroitin, 4% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-glucuronide + H2O
?
-
-
-
-
-
methylumbelliferyl-beta-D-glucuronide + H2O
methylumbelliferone + beta-D-glucuronate
-
-
-
-
?
N-([[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy]carbonyl)-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-met + H2O
D-glucuronate + N-[[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl]-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide
-
-
-
-
?
o-nitrophenyl-beta-D-glucuronide + H2O
o-nitrophenol + D-glucuronic acid
-
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
D-glucuronate + ?
-
1% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 192% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronic acid
-
-
-
-
?
phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + beta-D-glucuronate
-
determination of specific activity in betaG-expressing tumour CT26 cells using radio-labeled substrate
-
-
?
phenolphthalein-beta-D-glucuronic acid + H2O
phenolphthalein + D-glucuronic acid
-
-
-
-
ir
sodium (p-nitrophenyl beta-D-glucopyranoside)uronate + p-nitrophenyl alpha-D-galactopyranoside
p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside + p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside
-
the enzyme shows transglucuronidation activity: when (p-nitrophenyl beta-D-glucopyranoside)uronate is used as a donor and p-nitrophenyl alpha-D-galactopyranoside is used as an acceptor, p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside and p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside are synthesized in yields of 16% and 21% respectively
-
-
-
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
4-methyl-D-glucuronate + beta-Gal(1-6)Gal
-
-
42.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
4-methyl-D-glucuronate + beta-Gal(1-6)Gal
-
4.8% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
DQ459484
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
-
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
-
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-nitrophenol beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucoronate
-
-
-
?
4-nitrophenol beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucoronate
Aspergillus oryzae Li-3
-
-
-
?
4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
32% of the activity with 4-nitrophenyl beta-D-glucuronide
-
-
?
4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
-
-
?
4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
-
-
?
4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
32% of the activity with 4-nitrophenyl beta-D-glucuronide
-
-
?
4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
-
the enzyme shows transglucuronidation activity: when sodium (p-nitrophenyl beta-D-glucopyranoside)uronate is used as an acceptor and as a donor, sodium (sodium beta-D-glucopyranosyluronate)-(1-3)-(p-nitrophenyl beta-D-glucopyranosid)uronate and sodium (sodium beta-D-glucopyranosyluronate)-(1-2)-(p-nitrophenyl beta-D-glucopyranosid)uronate are obtained in yields of 18% and 15% respectively
-
-
-
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
-
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
low activity
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
low activity
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
-
-
?
4-nitrophenyl beta-D-glucuronic acid + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronic acid + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
best substrate
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
best substrate
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
-
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
-
-
?
baicalin + H2O
baicalein + beta-D-glucuronate
-
a major flavonoid derived from the root of Scutellaria baicalensis
productivity of 73%
-
?
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + H2O
glycyrrhetic acid + D-glucuronate
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
-
-
?
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + H2O
glycyrrhetic acid + D-glucuronate
Aspergillus oryzae Li-3
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
-
-
?
glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
-
specific substrate of isozymes I and II
-
-
?
glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
-
specific substrate of isozymes I and II
-
-
?
glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
-
-
-
?
glycyrrhizin + H2O
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + D-glucuronate
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
-
-
?
glycyrrhizin + H2O
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + D-glucuronate
Aspergillus oryzae Li-3
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
-
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
-
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
-
-
-
-
?
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
-
-
-
-
?
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
-
-
-
-
-
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
-
-
-
-
-
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
-
-
-
-
-
wogonin 7-O-beta-D-glucuronide + H2O
wogonin + beta-D-glucuronate
-
-
-
-
?
wogonin 7-O-beta-D-glucuronide + H2O
wogonin + beta-D-glucuronate
-
-
-
-
?
additional information
?
-
-
activity is slightly inducible by Met-Gly
-
-
-
additional information
?
-
-
enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose
-
-
-
additional information
?
-
the enzyme does not show strict specificity toward the aglycone moiety and exhibits activity toward all the natural and artificial substrates. Strict glycan specificity and hydrolyzes only the artificial substrate containing glucuronide groups
-
-
-
additional information
?
-
Aspergillus oryzae Li-3
the enzyme does not show strict specificity toward the aglycone moiety and exhibits activity toward all the natural and artificial substrates. Strict glycan specificity and hydrolyzes only the artificial substrate containing glucuronide groups
-
-
-
additional information
?
-
-
activity is not inducible by Met-Gly
-
-
-
additional information
?
-
-
role in physiology, in tissues, in body fluids
-
-
-
additional information
?
-
-
the potent beta-glucuronidase activity caused by the glucuronic acid conjugates from xenobiotics and endogenous compounds is a prime factor in the etiology of colon cancer
-
-
-
additional information
?
-
-
when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed
-
-
-
additional information
?
-
-
when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed
-
-
-
additional information
?
-
-
substrate specificity with flavonoids and phytoestrogens of crude and purified enzyme from digestive juice, overview
-
-
-
additional information
?
-
-
transfer of beta-glucuronosyl residues from aryl and alicyclic glucuronides to aliphatic alcohols and glycerols
-
-
-
additional information
?
-
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its retinoyl ester hydrolase activity
-
-
-
additional information
?
-
-
enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose
-
-
-
additional information
?
-
enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose
-
-
-
additional information
?
-
-
no activity with enzyme acting on chondroitin: N-acetylhyalobiuronic acid, N-acetylchondrosine, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2, GlcAbeta1-3galNAc(4-OSO3-), (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2. No activity with enzyme acting on p-nitrophenyl-beta-D-glucuronide: N-acetylhyalobiuronic acid, N-acetylchondrosine
-
-
-
additional information
?
-
-
the enzyme is involved in glycosaminoglycan metabolism
-
-
-
additional information
?
-
-
activity is not inducible by Met-Gly
-
-
-
additional information
?
-
-
activity is not inducible by Met-Gly
-
-
-
additional information
?
-
-
no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide
-
-
-
additional information
?
-
-
no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
curcumin-beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
curcumin, despite low systemic bioavailability, may be enzymatically activated (deconjugated) within GUSB-enriched bone to exert protective effects, a metabolic process that can also contribute to bone-protective effects of other highly glucuronidated dietary polyphenols
-
-
?
additional information
?
-
-
activity is slightly inducible by Met-Gly
-
-
-
additional information
?
-
-
activity is not inducible by Met-Gly
-
-
-
additional information
?
-
-
role in physiology, in tissues, in body fluids
-
-
-
additional information
?
-
-
the potent beta-glucuronidase activity caused by the glucuronic acid conjugates from xenobiotics and endogenous compounds is a prime factor in the etiology of colon cancer
-
-
-
additional information
?
-
-
when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed
-
-
-
additional information
?
-
-
when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed
-
-
-
additional information
?
-
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its retinoyl ester hydrolase activity
-
-
-
additional information
?
-
-
the enzyme is involved in glycosaminoglycan metabolism
-
-
-
additional information
?
-
-
activity is not inducible by Met-Gly
-
-
-
additional information
?
-
-
activity is not inducible by Met-Gly
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
K+
Mg2+
Na+
NaCl
additional information
K+
DQ459484
below 0.1 mM, recombinant enzyme expressed in Escherichia coli is activated
Mg2+
DQ459484
below 1 mM, recombinant enzyme expressed in Escherichia coli is activated
Na+
DQ459484
below 1 mM, recombinant enzyme expressed in Escherichia coli is activated
NaCl
-
below 0.075 M the activity of beta-glucuronidase acting on chondroitin is increased with increasing NaCl concentrations.The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition
additional information
-
Na+, K+, Mg2+, Mn2+, and Ca2+ increase the activity but with no significance
additional information
the catalytic activity of recombinant PGUS expressed under low-shear modeled microgravity is less affected by metal ions and EDTA as compared with that of normal gravity
additional information
-
the catalytic activity of recombinant PGUS expressed under low-shear modeled microgravity is less affected by metal ions and EDTA as compared with that of normal gravity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,1'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-diyl)bis(4,4,4-triethoxybut-2-en-1-one)
-
-
Al3+
DQ459484
10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
Ca2+
DQ459484
recombinant enzyme expressed in Escherichia coli is slightly inhibited
Cr3+
DQ459484
10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
Fe2+
DQ459484
1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
Fe3+
DQ459484
10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
Mn2+
DQ459484
1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
NaCl
-
between 0.075 and 0.25 M, the activity is decreased with increasing NaCl concentration. Above 0.25 M, the enzyme shows no activity. The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition
Pb2+
DQ459484
10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
S-saccharic acid 1,4-lactone
-
inhibits enzyme in several patients with colon cancer in addition to the healthy controls
2-deoxy-2-fluoro-beta-D-glucosyluronic acid fluoride
inactivation the recombinant His6-tagged enzyme
2-deoxy-2-fluoro-beta-D-glucosyluronic acid fluoride
mechanism-based inactivator, D-saccharic acid 1,4-lactone protects the enzyme against inactivation
azide
-
50 mM, 70% loss of activity of wild-type enzyme and mutant enzyme E451A. 50 mM-0.5 M, stimulation of mutant enzyme E451A. 1 mM, inhibition of wild-type enzyme and mutant enzyme E451A
Cu2+
DQ459484
1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
D-saccharic acid 1,4-lactone
competitive, protects the enzyme against inactivation by 2-deoxy-2-fluoro-beta-D-glucosyluronic acid fluoride
saccharic acid 1,4-lactone
80% inhibition by 0.5 mM, 88% inhibition by 1.0 mM
Zn2+
DQ459484
1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited
additional information
-
lower beta-glucuronidase activity is significantly associated with higher intakes of calcium, iron, and magnesium. Several dietary and nondietary factors are associated with beta-glucuronidase activity. The botanical families Cruciferae, Rutaceae, Compositae, Roseaceae, and Umbelliferae might decrease the enzyme activity when being part of the diet
-
additional information
-
substituted thieno[2,3-b]thiophenes and related congeners, synthesis of compounds incorporating the thieno[2,3-b]thiophene moiety, beta-glucuronidase inhibition activity, cytotoxicity, crystal structure, and Petra/Osiris/Molinspiration analyses, overview. No activity with 1,1'-(3-phenyl-4-methylthieno[2,3-b]thiene-2,5-diyl)bis[3-(dimethylamino)prop-2-en-1-one] and diethyl-4,4'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-dicarbonyl)bis(1-phenyl-1H-pyrazole-3-carboxylate)
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
abscisic acid
-
1.49fold increased enzyme activity after application of 100 micromo/l abscisic acid for 6 h
pectin-protein complex
-
adsorption of beta-glucuronidase on biopolymers is studied by the retention of the enzyme on the membrane of a concentrator with a pore diameter of 300 kDa and by native PAGE. Pectin-protein complexes (fractions PPC/CP and PPC/C) are established to increase the activity of beta-glucuronidase by 50 and 100%, respectively. There is a positive correlation between the increase of beta-glucuronidase activity in the presence of carbohydrates and enzyme adsorption on the polymers. The activity of the enzyme in the gel after electrophoresis of the PPC/+ beta-glucuronidase mixture is inversely proportional to the concentration of PPC/C in the mixture
-
salicylic acid
-
44.6% increase of enzyme activity after treatment with 1 mmol/l salicylic acid for 5 h
additional information
-
85.2% increased activity in response to 250 mmol/l NaCl for 8 h; increased expression and 1.87fold enzyme activity at dehydratation
-
additional information
-
higher beta-glucuronidase activity is significantly associated with being male, over 30 years old, non-Caucasian, overweighted, and exhibiting higher intakes of gamma-tocopherol. Several dietary and nondietary factors are associated with beta-glucuronidase activity
-
additional information
-
organophosphorous insecticides lead to several fold increased plasma enzyme activity, overview
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.25
4-methylumbelliferyl-beta-D-glucuronic acid
pH and temperature not specified in the publication
0.91
carboxyumbelliferyl-beta-D-glucuronide
-
pH 6.5, 38°C, Vmax: 0.041 mM/min
0.065
methylumbelliferyl-beta-D-glucuronide
-
pH 6.5, 38°C, Vmax: 0.026 mM/min
1.33
4-methylumbelliferyl-beta-D-glucuronide
-
37°C, pH 4.0-5.0, mutant enzyme E451A
3.06
4-nitrophenyl beta-D-galactosiduronide
pH 6.5, 75°C, recombinant His6-tagged enzyme
3.06
4-nitrophenyl beta-D-galactosiduronide
75°C, pH 6.5, wild-type enzyme
145
4-nitrophenyl beta-D-glucoside
pH 6.5, 75°C, recombinant His6-tagged enzyme
0.0046
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, mutant enzyme E383Q
0.0065
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, mutant enzyme E383A
0.083
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, wild-type enzyme
0.15
4-nitrophenyl beta-D-glucuronide
75°C, pH 6.5, wild-type enzyme
0.152
4-nitrophenyl beta-D-glucuronide
pH 6.5, 75°C, recombinant His6-tagged enzyme
0.414
4-nitrophenyl-beta-D-glucuronide
-
mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S
0.91
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme R563K
0.92
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, wild-type enzyme
1.06
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme V447Q
1.1
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme A365T
1.72
glycyrrhizin
pH 5, 55°C, low-shear modeled microgravity
additional information
additional information
kinetics of mutant enzymes, overview
-
additional information
additional information
-
kinetics of mutant enzymes, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.004
4-methylumbelliferyl-beta-D-glucuronide
-
37°C, pH 5.0, mutant enzyme E540A
0.015
4-methylumbelliferyl-beta-D-glucuronide
-
37°C, pH 4.0-5.0, mutant enzyme E451A
443
4-nitrophenyl beta-D-galactosiduronide
pH 6.5, 75°C, recombinant His6-tagged enzyme
443
4-nitrophenyl beta-D-galactosiduronide
75°C, pH 6.5, wild-type enzyme
9
4-nitrophenyl beta-D-glucoside
pH 6.5, 75°C, recombinant His6-tagged enzyme
0.33
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, mutant enzyme E383A
0.98
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, mutant enzyme E383Q
29
4-nitrophenyl beta-D-glucuronide
pH 6.5, 65°C, recombinant His6-tagged enzyme
29
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, wild-type enzyme
68
4-nitrophenyl beta-D-glucuronide
pH 6.5, 75°C, recombinant His6-tagged enzyme
68
4-nitrophenyl beta-D-glucuronide
75°C, pH 6.5, wild-type enzyme
1100
4-nitrophenyl-beta-D-glucuronide
-
mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S
0.89
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme R563K
3.32
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme V447Q
4.36
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme A365T
7.81
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, wild-type enzyme
23.3
glycyrrhizin
pH 5, 55°C, low-shear modeled microgravity
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
145
4-nitrophenyl beta-D-galactosiduronide
75°C, pH 6.5, wild-type enzyme
0.06
4-nitrophenyl beta-D-glucoside
75°C, pH 6.5, wild-type enzyme
51
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, mutant enzyme E383A
213
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, mutant enzyme E383Q
352
4-nitrophenyl beta-D-glucuronide
65°C, pH 6.5, wild-type enzyme
453
4-nitrophenyl beta-D-glucuronide
75°C, pH 6.5, wild-type enzyme
0.98
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme R563K
3.06
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme A365T
3.13
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, mutant enzyme V447Q
8.49
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
pH 5.0, 40°C, wild-type enzyme
13.55
glycyrrhizin
pH 5, 55°C, low-shear modeled microgravity
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.024
D-saccharic acid 1,4-lactone
pH 6.5, 75°C, recombinant His6-tagged enzyme
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0009
1,1'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-diyl)bis(4,4,4-triethoxybut-2-en-1-one)
Homo sapiens
-
pH not specified in the publication, 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.08
activity in crude cell extract from cell after growth for 9 h, activity-growth curve
29.3
additional information
additional information
-
the specific activity of the wild type and mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S are approximately 142.9 and 43.1 units/mg at 37°C and pH 6.5
additional information
-
activities of crude and purified enzyme from digestive juice
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.4
4 - 4.5
4.3
4.4
4.5
4.6
4.7
5.5
6.5
6.8
7.4
additional information
additional information
-
comparison of values for different substrates and organisms
additional information
-
comparison of values for different substrates and organisms
additional information
-
comparison of values for different substrates and organisms
additional information
-
comparison of values for different substrates and organisms
additional information
-
comparison of values for different substrates and organisms
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 5
4 - 5.5
4 - 7
pH 4.0: about 50% of maximal activity, pH 7.0: 25% of maximal activity
5 - 8.5
-
pH 5.0: about 45% of maximal activity, pH 8.5: about 50% of maximal activity
3 - 5
-
pH 3.0: about 50% of maximal activity, pH 5.0: about 70% of maximal activity, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide
4 - 5.5
-
pH 4.0: about 45% of maximal activity, pH 5.5: about 90% of maximal activity, pH 6.0: no activity, beta-glucuronidase acting on chondroitin
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
45
52
55
60
65
70
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37 - 100
37°C: about 40% of maximal activity, 100°C: about 50% of maximal activity
37 - 75
37°C: about 60% of maximal activity, 75°C: about 90% of maximal activity
38 - 70
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated from human intestinal microflora, gene uidA
SwissProt
brenda
isolated from human intestinal microflora, gene uidA
SwissProt
brenda
part of the normal flora of the respiratory and urogenital tracts of equines
UniProt
brenda
isolated from human intestinal microflora, two isozymes I and II
-
-
brenda
isolated from human intestinal microflora, two isozymes I and II
-
-
brenda
-
26854, 26874, 26875, 484862, 654242, 654243, 654393, 655150, 679012, 680580, 682636, 698525, 699502, 699925, 708894, 709548, 714869, 715238, 716508, 730451, 749576
-
-
brenda
-
26849, 26859, 26861, 26873, 654474, 654921, 655971, 656608, 679012, 682587, 697076, 697926, 698168, 710159, 729672, 730451, 731480, 739626
-
-
brenda
2 different exo-beta-glucuronidases: a beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide and a beta-glucuronidase acting on chondroitin
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
activity in round spermatids is detected in multivesicular bodies and acrosomal vesicles, activity in elongated spermatids is detected in sickle-shaped acrosome and in the residual body
brenda
additional information
additional information
-
beta-glucuronidase is a lysosomal enzyme that increases in the tissues of animals in response to xenobiotics entering the body
brenda
additional information
-
nearly all mammalian tissues and body fluids
brenda
additional information
-
distribution in tissues and organs; nearly all mammalian tissues and body fluids
brenda
additional information
-
CT26/betaG cell, colon carcinoma CT26 cells expressing beta-G
brenda
additional information
-
from armadillo liver infected with Mycobacterium leprae
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
activity in acrosome and in cytoplasmic droplets. Activity in round spermatids is detected in acrosomal vesicles, activity in elongated spermatids is detected in sickle-shaped acrosome
-
brenda
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its RE hydrolase activity
brenda
-
digestive juice, composition of the digestive juice containing enzyme and phytoestrogen compounds, overview
-
brenda
beta-glucuronidase is present in every compartment of the digestive cell endo-lysosomal compartment and appears to be not necessarily membrane-bound. Enzyme is released to the digestive alveolar lumen in secretory lysosomes produced by basophilic cells and endocytosed by digestive cells
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
-
beta-glucuronidase, an acid hydrolase that deconjugates glucuronides, may increase cancer risk
malfunction
mucopolysaccharidosis VII (MPS VII), called Sly syndrome, is caused by deficiency of the beta-glucuronidase
metabolism
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
metabolism
-
microbiome-encoded beta-glucuronidase enzymes play important roles in human health by metabolizing drugs in the gastrointestinal tract
-
physiological function
-
beta-glucuronidase forms a complex with esterase 22 in the liver, both esterase Es22 and Gus play a role in liver retinoid metabolism
physiological function
-
beta-glucuronidase, an acid hydrolase that deconjugates glucuronides, may increase cancer risk
Show AA Sequence and Transmembrane Helices (1963 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Clostridium perfringens (strain 13 / Type A)
Escherichia coli (strain K12)
Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)