Cloned (Comment) | Organism |
---|---|
gene TM1062, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain GMS407 | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
E383A | site-directed mutagenesis, the mutant enzyme shows reduced activity and similar thermal stability at 85°C compared to the wild-type enzyme | Thermotoga maritima |
E383Q | site-directed mutagenesis, the mutant enzyme shows reduced activity and thermal stability at 85°C compared to the wild-type enzyme | Thermotoga maritima |
E476A | site-directed mutagenesis, the mutant enzyme shows increased thermal stability at 85°C compared to the wild-type enzyme | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2-fluoro-beta-D-glucosyluronic acid fluoride | inactivation the recombinant His6-tagged enzyme | Thermotoga maritima | |
azide | - |
Thermotoga maritima | |
D-saccharic acid 1,4-lactone | competitive, protects the enzyme against inactivation by 2-deoxy-2-fluoro-beta-D-glucosyluronic acid fluoride | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of mutant enzymes, overview | Thermotoga maritima | |
0.152 | - |
4-nitrophenyl beta-D-glucuronide | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima | |
3.06 | - |
4-nitrophenyl beta-D-galactosiduronide | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima | |
145 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, recombinant His6-tagged enzyme, SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X0F2 | gene TM1062 | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | Q9X0F2 | gene TM1062 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain GMS407 by metal affinity chromatography to homogeneity | Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol | reaction mechanism, active site with catalytic nucleophile E576, residue E383 is also involved in catalysis | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactosiduronide + H2O | 32% of the activity with 4-nitrophenyl beta-D-glucuronide | Thermotoga maritima | 4-nitrophenol + beta-D-galactosiduronic acid | - |
? | |
4-nitrophenyl beta-D-galactosiduronide + H2O | 32% of the activity with 4-nitrophenyl beta-D-glucuronide | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-galactosiduronic acid | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | low activity | Thermotoga maritima | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | low activity | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucuronide + H2O | best substrate | Thermotoga maritima | 4-nitrophenol + beta-D-glucuronic acid | - |
? | |
4-nitrophenyl beta-D-glucuronide + H2O | best substrate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-glucuronic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, recombinant His6-tagged enzyme, SDS-PAGE | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
recombinant His6-tagged enzyme, half-life is 3 h | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima | |
29 | - |
4-nitrophenyl beta-D-glucuronide | pH 6.5, 65°C, recombinant His6-tagged enzyme | Thermotoga maritima | |
68 | - |
4-nitrophenyl beta-D-glucuronide | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima | |
443 | - |
4-nitrophenyl beta-D-galactosiduronide | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | - |
Thermotoga maritima |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermotoga maritima | |
0.024 | - |
D-saccharic acid 1,4-lactone | pH 6.5, 75°C, recombinant His6-tagged enzyme | Thermotoga maritima |