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Literature summary for 3.2.1.31 extracted from
- Effects of low-shear modeled microgravity on the characterization of recombinant beta-D-glucuronidase expressed in Pichia pastoris (2011), Appl. Biochem. Biotechnol., 163, 162-172.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| beta-D-glucuronidase gene derived from Penicillium purpurogenum Li-3 is expressed in Pichia pastoris GS115 in two different environments of low-shear modeled microgravity (LSMMG) and normal gravity (NG) | Talaromyces purpureogenus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Talaromyces purpureogenus |  |
| Cu2+ | - |
Talaromyces purpureogenus | |
| EDTA | - |
Talaromyces purpureogenus | |
| Zn2+ | - |
Talaromyces purpureogenus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.72 | - |
glycyrrhizin | pH 5, 55°C, low-shear modeled microgravity | Talaromyces purpureogenus | |
| 7.72 | - |
glycyrrhizin | pH 5, 55°C, normal gravity | Talaromyces purpureogenus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activity enhanced | Talaromyces purpureogenus | |
| Fe2+ | activity enhanced | Talaromyces purpureogenus | |
| K+ | activity enhanced | Talaromyces purpureogenus | |
| Mg2+ | activity enhanced | Talaromyces purpureogenus | |
| Mn2+ | activity enhanced | Talaromyces purpureogenus | |
| additional information | the catalytic activity of recombinant PGUS expressed under low-shear modeled microgravity is less affected by metal ions and EDTA as compared with that of normal gravity | Talaromyces purpureogenus | |
| Na+ | activity enhanced | Talaromyces purpureogenus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces purpureogenus | A7XS03 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycyrrhizin + H2O | - |
Talaromyces purpureogenus | 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-D-glucuronidase | - |
Talaromyces purpureogenus |
| PGUS | - |
Talaromyces purpureogenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Talaromyces purpureogenus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | - |
Talaromyces purpureogenus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.3 | - |
glycyrrhizin | pH 5, 55°C, low-shear modeled microgravity | Talaromyces purpureogenus | |
| 27.84 | - |
glycyrrhizin | pH 5, 55°C, normal gravity | Talaromyces purpureogenus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Talaromyces purpureogenus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Talaromyces purpureogenus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.61 | - |
glycyrrhizin | pH 5, 55°C, normal gravity | Talaromyces purpureogenus | |
| 13.55 | - |
glycyrrhizin | pH 5, 55°C, low-shear modeled microgravity | Talaromyces purpureogenus | |
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