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Literature summary for 3.2.1.31 extracted from
- Characterization of a thermostable beta-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana (2012), Appl. Microbiol. Biotechnol., 95, 1211-1219 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| butanol | 1%, 1.5fold activation | Thermotoga maritima |  |
| CTAB | 1%, 1.3fold activation | Thermotoga maritima | |
| Dimethylsulfoxide | 1%, 1.3fold activation | Thermotoga maritima | |
| EDTA | 1%, 1.25fold activation | Thermotoga maritima | |
| methanol | 1%, 1.4fold activation | Thermotoga maritima | |
| Triton X-100 | 1%, 1.9fold activation | Thermotoga maritima | |
| Tween-20 | 1%, 1.7fold activation | Thermotoga maritima | |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | use of beta-glucuronidase from Thermotoga maritima as a thermostable marker in higher plants, reporter enzyme in plant genetic research | Thermotoga maritima |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in both Escherichia coli and Arabidopsis thaliana. The enzyme expressed in transformed Escherichia coli cells exhibits maximum hydrolytic activity at 65°C and pH 6.5 and retains more than 80% activity after incubation at 85°C for 30 min. Activity in transgenic Arabidopsis thaliana plants containing TmGUSI is also stable over the temperature range 65-80°C | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| Ca2+ | recombinant enzyme expressed in Escherichia coli is slightly inhibited | Thermotoga maritima | |
| Citric acid | 1%, 42% inhibition | Thermotoga maritima | |
| Cr3+ | 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| Cu2+ | 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| dithiothreitol | 1%, 22% inhibition | Thermotoga maritima | |
| Fe2+ | 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| Fe3+ | 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| Mn2+ | 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| Pb2+ | 10 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
| SDS | 0.1% v/v, complete inhibition | Thermotoga maritima | |
| Urea | 1%, 38% inhibition | Thermotoga maritima | |
| Zn2+ | 1 mM, recombinant enzyme expressed in Escherichia coli is completely inhibited | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | below 0.1 mM, recombinant enzyme expressed in Escherichia coli is activated | Thermotoga maritima | |
| Mg2+ | below 1 mM, recombinant enzyme expressed in Escherichia coli is activated | Thermotoga maritima | |
| Na+ | below 1 mM, recombinant enzyme expressed in Escherichia coli is activated | Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | DQ459484 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-glucuronide + H2O | - |
Thermotoga maritima | 4-methylumbelliferone + D-glucuronic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65672, calculated from sequence | Thermotoga maritima |
| ? | x * 66000, enzyme expressed in Escherichia coli, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TmGUSI | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
enzyme expressed in Escherichia coli | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 80 | 30 min, enzyme expressed in Arabidopsis thaliana is stable | Thermotoga maritima |
| 85 | - |
30 min, enzyme expressed in Escherichia coli retains more than 80% of its activity | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
enzyme expressed in Escherichia coli | Thermotoga maritima |
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