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Literature summary for 3.2.1.31 extracted from

  • Kuroyama, H.; Tsutsui, N.; Hashimoto, Y.; Tsumuraya, Y.
    Purification and characterization of a beta-glucuronidase from Aspergillus niger (2001), Carbohydr. Res., 333, 27-39.
    View publication on PubMed
Search for more literature for 3.2.1.31

Inhibitors

Inhibitors Comment Organism Structure
D-glucaric acid 1,4-lactone 5 mM, 30% inhibition Aspergillus niger
Hg2+ 1 mM, 37°C, 10 min, 23% inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 p-nitrophenyl-beta-D-glucuronide pH 4.6, 37°C Aspergillus niger
0.53
-
 beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal pH 4.6, 37°C Aspergillus niger
0.71
-
 beta-GlcA-(1-6)-Gal pH 4.6, 37°C Aspergillus niger
1.4
-
 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal pH 4.6, 37°C Aspergillus niger
1.9
-
 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal pH 4.6, 37°C Aspergillus niger
3.6
-
 4-Me-beta-GlcA-(1-6)-Gal pH 4.6, 37°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 1 * 68000, SDS-PAGE Aspergillus niger
71000
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Pectinex Ultra SP-L, Lot No. G 770 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.6
-
 reaction with p-nitrophenyl-beta-D-glucuronide Aspergillus niger
29.3
-
 reaction with beta-GlcA-(1-6)-Gal Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
4-Me-beta-GlcA-(1-6)-Gal + H2O rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
alpha-L-arabinosidase-treated-arabinogalactan-protein + H2O
-
 Aspergillus niger ?
-
 ?
beta-GlcA-(1-3)-Gal + H2O rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ?
-
 ?
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger beta-Gal-(1-6)-Gal + beta-D-glucuronic acid
-
 ?
beta-GlcA-(1-6)-Gal + H2O rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger D-galactose + beta-D-glucuronic acid
-
 ?
additional information transglycosylation activity Aspergillus niger ?
-
 ?
p-nitrophenyl-beta-D-glucuronide + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-glucuronic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 4.6, 20 min, stable Aspergillus niger
75
-
 pH 4.6, 20 min, complete inactivation Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 reaction with beta-GlcA-(1-6)-Gal and p-nitrophenyl-beta-D-glucuronide Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 4°C, 24 h, stable Aspergillus niger
4.5 9 37°C, 3 h, stable Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing, pH-range: 3.5-10
-
 5