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Literature summary for 3.2.1.31 extracted from

  • Lin, C.W.; Orcutt, M.L.; Fishman, W.H.
    Purification and characterization of mouse kidney beta-glucuronidase (1975), J. Biol. Chem., 250, 4737-4743.
    View publication on PubMed
Search for more literature for 3.2.1.31

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018 3.08 phenolphthalein beta-D-glucuronide
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Mus musculus 5764
-
microsome
-
 Mus musculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000 310000 gradient polyacrylamide electrophoresis Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenolphthalein beta-D-glucuronide + H2O
-
 Mus musculus phenolphthalein + D-glucuronic acid
-
 ?
phenolphthalein-beta-D-glucuronide + H2O
-
 Mus musculus phenolphthalein + D-glucuronic acid
-
 ?

Subunits

Subunits Comment Organism
tetramer mouse, SDS-PAGE Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, inactivation at pH 5.0, not at pH 7.0 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
-
 Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11
-
 Mus musculus