Activating Compound | Comment | Organism | Structure |
---|---|---|---|
azide | 50 mM-0.5 M, stimulation of mutant enzyme E451A | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed COS cells and GUSB-deficient fibroblasts from the SV40 late promoter in vector pJC119 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D207A | 4.9% of the wild-type activity is expressed in COS cells | Homo sapiens |
E451A | 0.6% of the wild-type activity is expressed in COS cells, tetrameric enzyme, ratio of turnover number to KM-value is decreased 9100fold as compared to wild-type enzyme. Mutant enzyme E541A is inactivated at a faster rate than the wild-type enzyme, and is completely inactivated within 30 min. 50 mM azide causes 70% loss of activity of wild-type enzyme and mutant enzyme E451A. 50 mM-0.5 M stimulates mutant enzyme E451A. 1 mM inhibits wild-type enzyme and mutant enzyme E451A | Homo sapiens |
E451Q | 5.9% of the wild-type activity is expressed in COS cells | Homo sapiens |
E515A | no activity is expressed in COS cells | Homo sapiens |
E540A | no activity is expressed in COS cells, tetrameric enzyme, ratio of turnover number to KM-value is decreased 33000 fold as compared to wild-type enzyme, optimal pH is 5.0 instead of 4.5 for the wild-type enzyme. Mutant enzyme is inactivated at a faster rate than the wild-type enzyme at 68°C | Homo sapiens |
E540D | no activity is expressed in COS cells | Homo sapiens |
E540Q | 0.3% of the wild-type activity is expressed in COS cells | Homo sapiens |
Y504A | 1.4% of the wild-type activity is expressed in COS cells, tetrameric enzyme, the ratio of turnover number to KM-value is decreased 830fold as compared to wild-type enzyme. Mutant enzyme is as stable as the wild-type enzyme at 68°C | Homo sapiens |
Y504F | 2.3% of the wild-type activity is expressed in COS cells | Homo sapiens |
Y504H | 0.6% of the wild-type activity is expressed in COS cells | Homo sapiens |
Y508A | no activity is expressed in COS cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 50 mM, 70% loss of activity of wild-type enzyme and mutant enzyme E451A. 50 mM-0.5 M, stimulation of mutant enzyme E451A. 1 mM, inhibition of wild-type enzyme and mutant enzyme E451A | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, pH 5.0, mutant enzyme E540A | Homo sapiens | |
1.33 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, pH 4.0-5.0, mutant enzyme E451A | Homo sapiens | |
1.67 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, mutant enzyme Y540A | Homo sapiens | |
2.76 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, pH 4.5, wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucuronide + H2O | - |
Homo sapiens | 4-methylumbelliferone + D-glucuronic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hGUSB | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
pH 7.5, 75 mM NaCL and 5 mg/ml bovine serum albumin, 2 h: less than 40% inactivation of wild-type enzyme, mutant enzyme Y504A is as stable as the wild-type enzyme, mutant enzyme E540A is inactivated at a faster rate than the wild-type enzyme, mutant enzyme E541A is completely inactivated within 30 min | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, pH 5.0, mutant enzyme E540A | Homo sapiens | |
0.015 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, pH 4.0-5.0, mutant enzyme E451A | Homo sapiens | |
0.2 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, mutant enzyme Y540A | Homo sapiens | |
276 | - |
4-methylumbelliferyl-beta-D-glucuronide | 37°C, pH 4.5, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | mutant enzyme E451A | Homo sapiens |
4.5 | - |
wild-type enzyme | Homo sapiens |
5 | - |
mutant enzyme E540A | Homo sapiens |