Any feedback?
Information on EC 2.7.4.14 - UMP/CMP kinase
for references in articles please use BRENDA:EC2.7.4.14Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.4.14 UMP/CMP kinaseIUBMB Comments
This eukaryotic enzyme is a bifunctional enzyme that catalyses the phosphorylation of both CMP and UMP with similar efficiency. dCMP can also act as acceptor. Different from the monofunctional prokaryotic enzymes EC 2.7.4.25, (d)CMP kinase and EC 2.7.4.22, UMP kinase.
Specify your search results
Word Map
- 2.7.4.14
- dcmp
- deoxycytidine
- gemcitabine
- cidofovir
-
nmp-binding
- umpks
- medicine
- diagnostics
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
cmpk2, cmpk, cmp kinase, ump-cmp kinase, cmpk1, ump/cmp kinase, cytidylate kinase, pyrimidine nucleoside monophosphate kinase, cytidine monophosphate kinase, cytidine/uridine monophosphate kinase 2, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ATP:UMP-CMP phosphotransferase
-
-
-
-
CMP kinase
CMPK
CTP:CMP phosphotransferase
-
-
-
-
cytidine monophosphate kinase
cytidylate kinase
EC 2.7.4.5
-
-
formerly
-
kinase, cytidylate (phosphorylating)
-
-
-
-
MssA protein
-
-
-
-
nucleoside monophosphate kinase
P25
-
-
-
-
pyrimidine nucleoside monophosphate kinase
-
-
-
-
UCK
UMP-CMP kinase
UMP/CMP kinase
UMPK
additional information
CMP kinase
-
-
-
-
CMPK
-
-
-
-
cytidine monophosphate kinase
-
-
-
-
cytidylate kinase
-
-
-
-
UCK
-
-
-
-
UMP-CMP kinase
-
-
-
-
UMP/CMP kinase
-
-
-
-
UMPK
-
-
-
-
additional information
the enzyme belongs to the family of nucleoside monophosphate, NMP, kinases. These enzymes not only show high sequence and structure similarities but also share the alpha/beta-fold, a very common protein topology
additional information
mitochondrial UMP-CMPK2 belongs to a distinct nucleoside monophosphate kinase family, closer to thymidylate kinase than to cytosolic UMP-CMP kinase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + UMP = ADP + UDP
-
-
-
-
ATP + UMP = ADP + UDP
reaction mechanism is sequential and nonequilibrium in nature, substrates bind to the enzyme in a random order, substrate binding is cooperative
-
ATP + UMP = ADP + UDP
the mechanism is analogous to the phosphoryl transfer mechanism in cAMP-dependent protein kinase that phosphorylates the hydroxyl groups of serine residues
-
ATP + UMP = ADP + UDP
formation of a ternary complex, addition of substrates is random
-
ATP + UMP = ADP + UDP
reaction proceeds by a sequential mechanism, a ternary complex of the enzyme with both substrates is formed as the central intermediate in the reaction
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
ATP:CMP(UMP) phosphotransferase
This eukaryotic enzyme is a bifunctional enzyme that catalyses the phosphorylation of both CMP and UMP with similar efficiency. dCMP can also act as acceptor. Different from the monofunctional prokaryotic enzymes EC 2.7.4.25, (d)CMP kinase and EC 2.7.4.22, UMP kinase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37278-21-0
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine
ADP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine
Cidofovir
-
-
?
ATP + 2'-azido-CMP
ADP + 2'-azido-CDP
-
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
-
-
?
ATP + 2'-azido-dCMP
ADP + 2'-azido-dCDP
-
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
-
-
?
ATP + 2'-azido-dUMP
ADP + 2'-azido-dUDP
-
similar activity with 2'-azido-UMP and 2'-azido-dUMP
-
-
?
ATP + 2'-azido-UMP
ADP + 2'-azido-UDP
-
similar activity with 2'-azido-UMP and 2'-azido-dUMP
-
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate
-
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine monophosphate
activity of DCK
i.e. 2',2'-difluorodeoxycytidine 5'-monophosphate
-
?
ATP + gemcitabine phosphate
ADP + gemcitabine diphosphate
activity of CMPK
i.e. 2',2'-difluorodeoxycytidine 5'-diphosphate
-
?
ATP + 5-aza-cidofovir
ADP + phospho-5-aza-cidofovir
wild-type enzyme
-
-
?
ATP + cidofovir
ADP + phospho-cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
-
-
?
ATP + cidofovir
ADP + phospho-cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid, wild-type enzyme
-
-
?
ATP + CMP
ADP + CDP
-
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
-
-
?
ATP + CMP
ADP + CDP
-
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
-
-
?
ATP + cytarabine
ADP + phospho-cytarabine
anticancer drug, phosphorylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
-
-
?
ATP + cytarabine
ADP + phospho-cytarabine
i.e. araC, wild-type enzyme and mutant P64T
-
-
?
ATP + dCMP
ADP + dCDP
-
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
-
-
?
ATP + dCMP
ADP + dCDP
-
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
-
-
?
ATP + dCMP
ADP + dCDP
-
higher activity with the D-enantiomer compared to the L-enantiomer
-
-
?
ATP + dCMP
ADP + dCDP
-
no evidence for reversal of the reaction
-
?, ir
ATP + dUMP
ADP + dUDP
-
higher activity with the D-enantiomer compared to the L-enantiomer
-
-
?
ATP + gemcitabine
ADP + phospho-gemcitabine
i.e. 2',2'-difluorodeoxycytidine
-
-
?
ATP + gemcitabine
ADP + phospho-gemcitabine
i.e. 2',2'-difluorodeoxycytidine, wild-type enzyme
-
-
?
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
-
?
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
-
?
ATP + L-dCMP
ADP + L-dCDP
slower phosphorylation than the D-enantiomers
-
-
?
ATP + L-dUMP
ADP + L-dUDP
slower phosphorylation than the D-enantiomers
-
-
?
ATP + UMP
ADP + UDP
-
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
-
-
?
ATP + UMP
ADP + UDP
-
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
-
-
?
additional information
?
-
-
formation of a ternary complex, addition of substrates is random
-
-
?
additional information
?
-
-
the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site
-
-
?
additional information
?
-
the enzyme is involved in the phosphorylation of nucleic acid precursors, and is critical in the ribo- and deoxyribonucleoside salvage pathway, as well as the anabolic phosphorylation of nucleoside analogues used in viral and anticancer therapies, e.g. cidofovir, which is activated by the enzyme, overview
-
-
?
additional information
?
-
-
the enzyme is involved in the phosphorylation of nucleic acid precursors, and is critical in the ribo- and deoxyribonucleoside salvage pathway, as well as the anabolic phosphorylation of nucleoside analogues used in viral and anticancer therapies, e.g. cidofovir, which is activated by the enzyme, overview
-
-
?
additional information
?
-
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
-
-
?
additional information
?
-
-
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
-
-
?
additional information
?
-
substrate/product binding site determination and kinetics using MABA-CDP in a competition assay, overview
-
-
?
additional information
?
-
-
substrate/product binding site determination and kinetics using MABA-CDP in a competition assay, overview
-
-
?
additional information
?
-
-
the enzyme shows enantioselectivity, acceptor-binding site structure of human UMP-CMP kinase in the closed form or in ligand-bound forms, overview
-
-
?
additional information
?
-
hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview
-
-
?
additional information
?
-
CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP
-
-
?
additional information
?
-
mutants P64T/R134M and R134M show no activity with all substrates
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + cidofovir
ADP + phospho-cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
-
-
?
ATP + cytarabine
ADP + phospho-cytarabine
anticancer drug, phosphorylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
-
-
?
ATP + gemcitabine
ADP + phospho-gemcitabine
i.e. 2',2'-difluorodeoxycytidine
-
-
?