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Literature summary for 2.7.4.14 extracted from

  • Xu, Y.; Johansson, M.; Karlsson, A.
    Human UMP-CMP kinase 2, a novel nucleoside monophosphate kinase localized in mitochondria (2008), J. Biol. Chem., 283, 1563-1571.
    View publication on PubMed
Search for more literature for 2.7.4.14

Cloned(Commentary)

Cloned (Comment) Organism
UMP-CMPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His6-tagged mutant UMP-CMPK2DELTA21, lacking the mitochondrial targeting sequence, in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, expression of GFP-tagged wild-type and E448G mutant enzymes in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E448G site-directed mutagenesis Homo sapiens
additional information construction of a UMP-CMPK2DELTA21 mutant lacking the mitochondrial targeting sequence Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 dUMP pH 8.0, 37°C, wild-type enzyme Homo sapiens
1.31
-
 dCMP pH 8.0, 37°C, wild-type enzyme Homo sapiens
3.09
-
 CMP pH 8.0, 37°C, wild-type enzyme Homo sapiens
6.3
-
 UMP pH 8.0, 37°C, wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion UMP-CMPK2 contains an N-terminal mitochondrial targeting sequence Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5EBM0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged mutant UMP-CMPK2DELTA21 from Spodoptera frugiperda Sf9 cells by affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
K-562 cell chronic myelogenous leukemia cell line Homo sapiens
-
leukemia cell high expression Homo sapiens
-
macrophage
-
 Homo sapiens
-
MOLT-4 cell lymphoblastic leukemia cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CMP low activity Homo sapiens ADP + CDP
-
 ?
ATP + dCMP high activity Homo sapiens ADP + dCDP
-
 ?
ATP + dUMP best substrate Homo sapiens ADP + dUDP
-
 ?
ATP + UMP low activity Homo sapiens ADP + UDP
-
 ?
additional information UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
More mitochondrial UMP-CMPK2 belongs to a distinct nucleoside monophosphate kinase family, closer to thymidylate kinase than to cytosolic UMP-CMP kinase Homo sapiens
nucleoside monophosphate kinase
-
 Homo sapiens
UMP-CMP kinase 2
-
 Homo sapiens
UMP-CMPK2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens