BRENDA - Enzyme Database show
show all sequences of 2.7.4.14

Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates

Liou, J.Y.; Dutschman, G.E.; Lam, W.; Jiang, Z.; Cheng, Y.C.; Cancer Res. 62, 1624-1631 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
CTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
dCTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
TTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
UTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
CMP
37°C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.023
-
dCMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.067
-
UMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.069
-
UMP
37°C, 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.204
-
ATP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.211
-
CMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.228
-
beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
37°C
Homo sapiens
0.25
-
L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
37°C, His-tagged UMP/CMP kinase
Homo sapiens
0.272
-
beta-D-2',3'-dideoxy-CMP
37°C
Homo sapiens
0.354
-
dCMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.494
-
L-(-)-2',3'-dideoxy-3'-thia-CMP
37°C
Homo sapiens
0.513
-
dCMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.581
-
gemcitabine monophosphate
37°C
Homo sapiens
0.697
-
beta-L-2',3'-dideoxy-CMP
37°C
Homo sapiens
0.917
-
ara-CMP
37°C
Homo sapiens
1.037
-
beta-D-2',3'-dideoxy-CMP
37°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + CMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + CDP
-
-
?
ATP + dCMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + dCDP
-
-
?
ATP + UMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + UDP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HeLa-S3 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + ara-CMP
-
645161
Homo sapiens
ADP + ara-CDP
-
-
-
?
ATP + beta-D-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + beta-L-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + beta-L-dioxolane-cytidine
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + CMP
-
645161
Homo sapiens
ADP + CDP
-
-
-
?
ATP + CMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + CDP
-
-
-
?
ATP + dCMP
-
645161
Homo sapiens
ADP + dCDP
-
-
-
?
ATP + dCMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + dCDP
-
-
-
?
ATP + gemcitabine monophosphate
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
645161
Homo sapiens
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
?
ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + UMP
-
645161
Homo sapiens
ADP + UDP
-
-
-
?
ATP + UMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + UDP
-
-
-
?
CTP + CMP
-
645161
Homo sapiens
CDP + CDP
-
-
-
?
dATP + CMP
-
645161
Homo sapiens
dADP + CDP
-
-
-
?
dATP + UMP
-
645161
Homo sapiens
dADP + ADP
-
-
-
?
dCTP + dCMP
-
645161
Homo sapiens
dCDP + dCDP
-
-
-
?
dCTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
dGTP + CMP
-
645161
Homo sapiens
dGDP + CDP
-
-
-
?
dGTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
GTP + CMP
-
645161
Homo sapiens
GDP + CDP
-
-
-
?
GTP + dCMP
-
645161
Homo sapiens
GDP + dCDP
-
-
-
?
GTP + UMP
-
645161
Homo sapiens
GDP + UDP
-
-
-
?
TTP + CMP
-
645161
Homo sapiens
TDP + CDP
-
-
-
?
TTP + dCMP
-
645161
Homo sapiens
TDP + dCMP
-
-
-
?
TTP + UMP
-
645161
Homo sapiens
TDP + UDP
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
dCTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
TTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
UTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
CMP
37°C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.023
-
dCMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.067
-
UMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.069
-
UMP
37°C, 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.204
-
ATP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.211
-
CMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.228
-
beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
37°C
Homo sapiens
0.25
-
L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
37°C, His-tagged UMP/CMP kinase
Homo sapiens
0.272
-
beta-D-2',3'-dideoxy-CMP
37°C
Homo sapiens
0.354
-
dCMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.494
-
L-(-)-2',3'-dideoxy-3'-thia-CMP
37°C
Homo sapiens
0.513
-
dCMP
37°C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
0.581
-
gemcitabine monophosphate
37°C
Homo sapiens
0.697
-
beta-L-2',3'-dideoxy-CMP
37°C
Homo sapiens
0.917
-
ara-CMP
37°C
Homo sapiens
1.037
-
beta-D-2',3'-dideoxy-CMP
37°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + CMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + CDP
-
-
?
ATP + dCMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + dCDP
-
-
?
ATP + UMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + UDP
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HeLa-S3 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + ara-CMP
-
645161
Homo sapiens
ADP + ara-CDP
-
-
-
?
ATP + beta-D-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + beta-L-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + beta-L-dioxolane-cytidine
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + CMP
-
645161
Homo sapiens
ADP + CDP
-
-
-
?
ATP + CMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + CDP
-
-
-
?
ATP + dCMP
-
645161
Homo sapiens
ADP + dCDP
-
-
-
?
ATP + dCMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + dCDP
-
-
-
?
ATP + gemcitabine monophosphate
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
645161
Homo sapiens
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
?
ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
ATP + UMP
-
645161
Homo sapiens
ADP + UDP
-
-
-
?
ATP + UMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + UDP
-
-
-
?
CTP + CMP
-
645161
Homo sapiens
CDP + CDP
-
-
-
?
dATP + CMP
-
645161
Homo sapiens
dADP + CDP
-
-
-
?
dATP + UMP
-
645161
Homo sapiens
dADP + ADP
-
-
-
?
dCTP + dCMP
-
645161
Homo sapiens
dCDP + dCDP
-
-
-
?
dCTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
dGTP + CMP
-
645161
Homo sapiens
dGDP + CDP
-
-
-
?
dGTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
GTP + CMP
-
645161
Homo sapiens
GDP + CDP
-
-
-
?
GTP + dCMP
-
645161
Homo sapiens
GDP + dCDP
-
-
-
?
GTP + UMP
-
645161
Homo sapiens
GDP + UDP
-
-
-
?
TTP + CMP
-
645161
Homo sapiens
TDP + CDP
-
-
-
?
TTP + dCMP
-
645161
Homo sapiens
TDP + dCMP
-
-
-
?
TTP + UMP
-
645161
Homo sapiens
TDP + UDP
-
-
-
?
Other publictions for EC 2.7.4.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739215
Topalis
Resistance to the nucleotide a ...
Homo sapiens
Oncotarget
7
10386-10401
2016
-
-
1
-
3
-
-
11
1
1
-
9
-
3
-
-
-
-
-
3
-
-
18
-
-
-
1
11
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
11
1
1
-
9
-
-
-
-
-
3
-
-
18
-
-
-
1
11
-
-
-
-
-
4
4
-
11
11
739700
Liu
Prognostic significance of nuc ...
Homo sapiens
Sci. Rep.
6
32027
2016
-
1
1
-
-
-
-
-
2
1
1
2
-
2
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
2
1
1
2
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
737991
Tsao
The contribution of CMP kinase ...
Homo sapiens
Cell Cycle
14
354-363
2015
-
-
-
-
1
-
-
-
1
1
-
2
-
1
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
702166
Liou
Modulation of human UMP/CMP ki ...
Homo sapiens
Biochem. Pharmacol.
79
381-388
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692040
Kocabas
Gemcitabine pharmacogenomics: ...
Homo sapiens
Drug Metab. Dispos.
36
1951-1959
2008
-
-
1
-
1
-
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693063
Xu
Human UMP-CMP kinase 2, a nove ...
Homo sapiens
J. Biol. Chem.
283
1563-1571
2008
-
-
1
-
2
-
-
4
2
1
-
1
-
2
-
-
1
-
-
6
-
-
5
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
4
2
1
-
1
-
-
-
1
-
6
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
693656
Lorenz
The influence of proline isome ...
Dictyostelium discoideum
J. Mol. Biol.
381
443-455
2008
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673598
Topalis
Nucleotide binding to human UM ...
Homo sapiens
FEBS J.
274
3704-3714
2007
-
1
1
1
-
-
6
5
-
1
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
4
-
6
-
1
1
-
1
-
-
6
6
4
5
-
1
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676227
Alexandre
Enantioselectivity of human AM ...
Homo sapiens
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
-
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Activation of rat liver pyrimi ...
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Gravey
ATP:UMP-CMP phosphotransferase ...
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