BRENDA - Reference to 2.7.4.14; Id = 645158

G21A

mutant enzyme is degraded during the purification phase

Arabidopsis thaliana

G22A

mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 59% of that of the wild-type enzyme

Arabidopsis thaliana

G24A

mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 48% of that of the wild-type enzyme

Arabidopsis thaliana

G26A

mutant enzyme with decreased turnover-number/KmATP value

Arabidopsis thaliana

G27R

mutant enzyme is degraded during the purification phase. Turnover-number is 45% of that of the wild-type enzyme

Arabidopsis thaliana

K27E

mutant enzyme with 2600fold decreased turnover-number/KmATP value. Turnover-number is 21% of that of the wild-type enzyme

Arabidopsis thaliana

K27M

mutant enzyme with 1000fold decreased turnover-number/KmATP value. Turnover-number is 22% of that of the wild-type enzyme

Arabidopsis thaliana

-

ATP + CMP

-

645158

Arabidopsis thaliana

ADP + CDP

-

?

ATP + UMP

-

645158

Arabidopsis thaliana

ADP + UDP

-

?

G21A

mutant enzyme is degraded during the purification phase

Arabidopsis thaliana

G22A

mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 59% of that of the wild-type enzyme

Arabidopsis thaliana

G24A

mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 48% of that of the wild-type enzyme

Arabidopsis thaliana

G26A

mutant enzyme with decreased turnover-number/KmATP value

Arabidopsis thaliana

G27R

mutant enzyme is degraded during the purification phase. Turnover-number is 45% of that of the wild-type enzyme

Arabidopsis thaliana

K27E

mutant enzyme with 2600fold decreased turnover-number/KmATP value. Turnover-number is 21% of that of the wild-type enzyme

Arabidopsis thaliana

K27M

mutant enzyme with 1000fold decreased turnover-number/KmATP value. Turnover-number is 22% of that of the wild-type enzyme

Arabidopsis thaliana

ATP + CMP

-

645158

Arabidopsis thaliana

ADP + CDP

-

?

ATP + UMP

-

645158

Arabidopsis thaliana

ADP + UDP

-

?

739215

Topalis

Resistance to the nucleotide a ...

Homo sapiens

Oncotarget

7

10386-10401

2016

-

1

-

3

-

11

1

-

9

-

3

-

3

-

18

-

1

11

-

1

-

1

-

3

-

11

1

-

9

-

3

-

18

-

1

11

-

4

-

11

739700

Liu

Prognostic significance of nuc ...

Homo sapiens

Sci. Rep.

6

32027

2016

-

1

-

2

1

2

-

2

-

3

-

2

1

-

1

-

1

-

2

1

2

-

3

-

2

1

-

2

-

737991

Tsao

The contribution of CMP kinase ...

Homo sapiens

Cell Cycle

14

354-363

2015

-

1

-

1

-

2

-

1

-

2

-

7

-

1

-

1

-

1

-

1

-

2

-

2

-

7

-

3

-

702166

Liou

Modulation of human UMP/CMP ki ...

Homo sapiens

Biochem. Pharmacol.

79

381-388

2010

-

1

-

2

-

7

-

1

-

7

-

692040

Kocabas

Gemcitabine pharmacogenomics: ...

Homo sapiens

Drug Metab. Dispos.

36

1951-1959

2008

-

1

-

1

-

3

-

1

-

2

-

2

1

-

1

-

1

-

1

-

3

-

1

-

2

1

-

693063

Xu

Human UMP-CMP kinase 2, a nove ...

Homo sapiens

J. Biol. Chem.

283

1563-1571

2008

-

1

-

2

-

4

2

1

-

1

-

2

-

1

-

6

-

5

-

1

-

1

-

1

-

1

-

2

-

4

2

1

-

1

-

1

-

6

-

5

-

1

-

1

-

693656

Lorenz

The influence of proline isome ...

Dictyostelium discoideum

J. Mol. Biol.

381

443-455

2008

-

1

-

3

-

1

-

2

-

1

-

1

-

2

-

673598

Topalis

Nucleotide binding to human UM ...

Homo sapiens

FEBS J.

274

3704-3714

2007

-

1

-

6

5

-

1

-

2

-

1

-

5

-

4

-

6

-

1

-

1

-

6

4

5

-

1

-

1

-

5

-

676227

Alexandre

Enantioselectivity of human AM ...

Homo sapiens

Nucleic Acids Res.

35

4895-4904

2007

-

1

-

1

12

-

2

-

1

-

12

-

1

-

1

-

12

-

1

-

12

-

694478

Topalis

Looking for new pyrimidine acy ...

Homo sapiens

Nucleosides Nucleotides Nucleic Acids

26

1369-1373

2007

-

1

-

3

-

2

-

4

-

1

-

1

-

1

-

3

-

4

-

694479

Alexandre

Enantio-selectivity of human n ...

Homo sapiens

Nucleosides Nucleotides Nucleic Acids

26

1375-1379

2007

-

11

-

1

-

4

-

1

-

12

-

1

-

1

-

1

-

1

-

11

-

1

-

4

-

12

-

1

-

1

-

663161

Hewitt

Effects of phosphate limitatio ...

Arabidopsis thaliana

Plant Physiol. Biochem.

43

91-99

2005

-

1

-

1

-

1

-

645155

Curbo

The Drosophila melanogaster UM ...

Drosophila melanogaster

Biochem. Biophys. Res. Commun.

311

440-445

2003

-

1

-

4

2

-

4

-

4

-

1

-

4

2

-

4

-

645162

Pasti

Reaction of human UMP-CMP kina ...

Dictyostelium sp., Homo sapiens

Eur. J. Biochem.

270

1784-1790

2003

-

1

-

2

16

-

3

-

4

-

12

-

14

-

4

-

1

-

2

4

16

-

3

-

12

-

14

-

645161

Liou

Characterization of human UMP/ ...

Homo sapiens

Cancer Res.

62

1624-1631

2002

-

1

-

4

16

1

-

3

-

3

-

1

-

1

-

27

-

1

-

4

-

16

1

-

3

-

1

-

1

-

27

-

645160

Pearman

Characterization of human UMP- ...

Homo sapiens

Life Sci.

69

2361-2370

2001

-

1

-

2

-

21

-

3

1

-

1

-

21

-

3

1

-

645152

Hutter

Phosphoryl transfer by a conce ...

Dictyostelium discoideum

Protein Sci.

9

2225-2231

2000

-

1

-

1

-

1

-

1

-

645156

Van Rompay

Phosphorylation of deoxycytidi ...

Homo sapiens

Mol. Pharmacol.

56

562-569

1999

-

1

-

5

2

-

3

-

9

-

1

-

5

2

-

9

-

645153

Zhou

Cloning, expression in Escheri ...

Arabidopsis thaliana

Plant Physiol.

117

245-254

1998

-

1

-

1

4

-

1

-

5

-

1

-

2

1

-

1

2

1

-

2

-

1

-

1

2

4

-

1

-

1

-

2

1

-

1

2

1

-

645157

Okajima

Exchange of nucleoside monopho ...

Sus scrofa

J. Biochem.

124

359-367

1998

-

1

-

2

-

3

-

3

-

2

-

3

-

1

-

2

-

3

-

2

-

3

-

645158

Zhou

Site-specific mutations of con ...

Arabidopsis thaliana

Arch. Biochem. Biophys.

358

297-302

1998

-

7

-

2

-

2

-

7

-

2

-

645150

Seagrave

Pyrimidine nucleoside monophos ...

Rattus norvegicus

Arch. Biochem. Biophys.

254

518-525

1987

-

2

4

-

2

-

1

-

3

-

2

-

4

-

3

-

645147

Seagrave

Pyrimidine nucleoside monophos ...

Rattus norvegicus

Arch. Biochem. Biophys.

247

76-83

1986

-

2

5

-

2

-

2

-

3

-

2

-

1

-

1

-

2

-

5

-

2

-

3

-

2

-

1

-

1

-

209853

Neale

Enzymes of pyrimidine deoxyrib ...

Mycoplasma mycoides

J. Bacteriol.

156

1001-1005

1983

-

1

-

3

-

3

-

645149

Kohno

Purification and properties of ...

Saccharomyces cerevisiae

Agric. Biol. Chem.

47

19-24

1983

1

-

5

4

-

3

1

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

5

-

4

-

3

1

-

1

-

1

-

3

-

1

-

1

-

645141

Scott

Kinetics and equilibria of pyr ...

Homo sapiens

Biochim. Biophys. Acta

571

45-54

1979

-

2

-

1

-

2

-

1

-

2

1

5

-

1

-

2

-

2

-

1

-

1

-

2

1

5

-

1

-

2

-

645139

Orengo

Pyrimidine nucleoside monophos ...

Rattus norvegicus

Methods Enzymol.

51

321-331

1978

1

-

5

18

-

6

-

2

-

1

-

4

1

8

-

1

-

5

-

18

-

6

-

1

-

4

1

8

-

645140

Anderson

UMP-CMP kinase from Tetrahymen ...

Tetrahymena pyriformis

Methods Enzymol.

51

331-337

1978

-

1

3

-

2

-

1

-

1

-

4

-

1

-

1

-

1

-

3

-

1

-

1

-

4

-

1

-

645144

Kobayashi

NADPH activation of deoxycytid ...

Rattus norvegicus

Biochem. Biophys. Res. Commun.

74

1249-1255

1977

1

-

1

-

1

-

1

-

1

-

645142

Maness

Activation of rat liver pyrimi ...

Rattus norvegicus

Biochim. Biophys. Acta

429

182-190

1976

2

-

1

-

2

-

2

-

2

-

1

-

1

-

2

-

645146

Teng

Human erythrocyte pyrimidine n ...

Homo sapiens

J. Biol. Chem.

251

4179-4183

1976

-

4

6

-

2

-

1

-

2

-

4

-

4

-

4

-

6

-

1

-

2

-

4

-

4

-

645143

Maness

A pyrimidine nucleoside monoph ...

Rattus norvegicus

Biochemistry

14

1484-1489

1975

6

-

2

6

-

2

-

2

-

1

-

2

1

-

16

-

6

-

2

-

6

-

2

-

1

-

2

1

-

16

-

645148

Gravey

ATP:UMP-CMP phosphotransferase ...

Tetrahymena pyriformis

Biochim. Biophys. Acta

302

38-49

1973

-

1

-

2

-

1

-

1

-

645137

Ruffner

Adenosine triphosphate: uridin ...

Tetrahymena pyriformis

J. Biol. Chem.

244

5994-6002

1969

-

2

5

-

1

-

1

-

1

3

-

1

-

2

-

5

-

1

-

1

3

-

1

-

645138

Hurwitz

The enzymatic incorporation of ...

Escherichia coli, Escherichia coli B / ATCC 11303

J. Biol. Chem.

234

2351-2358

1959

-

177

-

1

-

1

4

-

1

-

1

4

-

Site-specific mutations of conserved residues in the phosphate-binding loop of the Arabidopsis UMP/CMP kinase alter ATP and UMP binding

Data extracted from this reference: