BRENDA - Enzyme Database show
show all sequences of 2.7.4.14

Characterization of human UMP-CMP kinase enzymatic activity and 5' untranslated region

Pearman, A.T.; Castro-Faria-Neto, H.C.; McIntyre, T.M.; Prescott, S.M.; Stafforini, D.M.; Life Sci. 69, 2361-2370 (2001)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
strong requirement, maximal activity at 3 mM
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22222
-
x * 22222, calculation from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + CMP
Homo sapiens
the enzyme is required during rapid cell growth
ADP + CDP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
A-549 cell
lung carcinoma
Homo sapiens
-
bone marrow
-
Homo sapiens
-
Burkitt lymphoma cell
-
Homo sapiens
-
fetus
-
Homo sapiens
-
G-361 cell
melanoma cell
Homo sapiens
-
HeLa-S3 cell
-
Homo sapiens
-
K-562 cell
chronic myelogenous leukemia cell
Homo sapiens
-
leukocyte
-
Homo sapiens
-
liver
-
Homo sapiens
-
lymph node
-
Homo sapiens
-
macrophage
-
Homo sapiens
-
MOLT-4 cell
lymphoblastic leukemia cell
Homo sapiens
-
additional information
two mRNA species are expressed in all immune tissues examined, an all cases the 3.4 kb form is the more prominent RNA species
Homo sapiens
-
NCI-H660 cell
promyelocytic leukemia
Homo sapiens
-
peripheral blood mononuclear cell
-
Homo sapiens
-
RAJI cell
-
Homo sapiens
-
spleen
-
Homo sapiens
-
SW-480 cell
colorectal adenocarcinoma cell
Homo sapiens
-
thymus
-
Homo sapiens
-
vermiform appendix
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + CMP
-
645160
Homo sapiens
ADP + CDP
-
-
-
?
ATP + CMP
the enzyme is required during rapid cell growth
645160
Homo sapiens
ADP + CDP
-
-
-
?
ATP + UMP
-
645160
Homo sapiens
ADP + UDP
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 22222, calculation from nucleotide sequence
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
strong requirement, maximal activity at 3 mM
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22222
-
x * 22222, calculation from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + CMP
Homo sapiens
the enzyme is required during rapid cell growth
ADP + CDP
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
A-549 cell
lung carcinoma
Homo sapiens
-
bone marrow
-
Homo sapiens
-
Burkitt lymphoma cell
-
Homo sapiens
-
fetus
-
Homo sapiens
-
G-361 cell
melanoma cell
Homo sapiens
-
HeLa-S3 cell
-
Homo sapiens
-
K-562 cell
chronic myelogenous leukemia cell
Homo sapiens
-
leukocyte
-
Homo sapiens
-
liver
-
Homo sapiens
-
lymph node
-
Homo sapiens
-
macrophage
-
Homo sapiens
-
MOLT-4 cell
lymphoblastic leukemia cell
Homo sapiens
-
additional information
two mRNA species are expressed in all immune tissues examined, an all cases the 3.4 kb form is the more prominent RNA species
Homo sapiens
-
NCI-H660 cell
promyelocytic leukemia
Homo sapiens
-
peripheral blood mononuclear cell
-
Homo sapiens
-
RAJI cell
-
Homo sapiens
-
spleen
-
Homo sapiens
-
SW-480 cell
colorectal adenocarcinoma cell
Homo sapiens
-
thymus
-
Homo sapiens
-
vermiform appendix
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + CMP
-
645160
Homo sapiens
ADP + CDP
-
-
-
?
ATP + CMP
the enzyme is required during rapid cell growth
645160
Homo sapiens
ADP + CDP
-
-
-
?
ATP + UMP
-
645160
Homo sapiens
ADP + UDP
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 22222, calculation from nucleotide sequence
Homo sapiens
Other publictions for EC 2.7.4.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739215
Topalis
Resistance to the nucleotide a ...
Homo sapiens
Oncotarget
7
10386-10401
2016
-
-
1
-
3
-
-
11
1
1
-
9
-
3
-
-
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-
-
3
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-
18
-
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-
1
11
-
-
-
1
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-
1
1
-
3
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11
1
1
-
9
-
-
-
-
-
3
-
-
18
-
-
-
1
11
-
-
-
-
-
4
4
-
11
11
739700
Liu
Prognostic significance of nuc ...
Homo sapiens
Sci. Rep.
6
32027
2016
-
1
1
-
-
-
-
-
2
1
1
2
-
2
-
-
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-
-
3
-
-
2
1
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-
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-
-
-
-
1
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1
1
1
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-
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2
1
1
2
-
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-
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3
-
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2
1
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-
-
-
-
-
-
-
-
2
2
-
-
-
737991
Tsao
The contribution of CMP kinase ...
Homo sapiens
Cell Cycle
14
354-363
2015
-
-
-
-
1
-
-
-
1
1
-
2
-
1
-
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-
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2
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7
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1
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1
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1
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1
1
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2
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2
-
-
7
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
702166
Liou
Modulation of human UMP/CMP ki ...
Homo sapiens
Biochem. Pharmacol.
79
381-388
2010
-
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1
-
-
-
-
-
-
-
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2
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-
7
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1
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7
-
-
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-
-
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-
-
-
-
692040
Kocabas
Gemcitabine pharmacogenomics: ...
Homo sapiens
Drug Metab. Dispos.
36
1951-1959
2008
-
-
1
-
1
-
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
1
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-
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1
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1
1
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1
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-
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3
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1
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-
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693063
Xu
Human UMP-CMP kinase 2, a nove ...
Homo sapiens
J. Biol. Chem.
283
1563-1571
2008
-
-
1
-
2
-
-
4
2
1
-
1
-
2
-
-
1
-
-
6
-
-
5
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
4
2
1
-
1
-
-
-
1
-
6
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
693656
Lorenz
The influence of proline isome ...
Dictyostelium discoideum
J. Mol. Biol.
381
443-455
2008
-
-
-
-
-
-
-
-
-
-
1
-
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3
-
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1
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2
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-
-
-
-
-
-
673598
Topalis
Nucleotide binding to human UM ...
Homo sapiens
FEBS J.
274
3704-3714
2007
-
1
1
1
-
-
6
5
-
1
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
4
-
6
-
1
1
-
1
-
-
6
6
4
5
-
1
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676227
Alexandre
Enantioselectivity of human AM ...
Homo sapiens
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
-
-
-
1
12
-
-
-
-
-
2
-
-
1
-
-
-
-
-
12
-
-
-
-
-
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-
-
-
-
-
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-
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1
-
-
-
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1
-
12
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694478
Topalis
Looking for new pyrimidine acy ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
26
1369-1373
2007
-
-
-
-
-
-
-
-
1
1
-
3
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
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-
1
-
-
-
-
-
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-
1
1
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3
-
-
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-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694479
Alexandre
Enantio-selectivity of human n ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
26
1375-1379
2007
-
-
-
-
-
-
-
11
-
1
-
4
-
1
-
-
-
-
-
-
-
-
12
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
1
-
4
-
-
-
-
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
663161
Hewitt
Effects of phosphate limitatio ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
91-99
2005
-
-
-
-
-
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-
-
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1
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1
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1
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-
645155
Curbo
The Drosophila melanogaster UM ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
311
440-445
2003
-
-
1
-
-
-
-
4
2
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-
-
-
4
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4
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1
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4
2
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-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645162
Pasti
Reaction of human UMP-CMP kina ...
Dictyostelium sp., Homo sapiens
Eur. J. Biochem.
270
1784-1790
2003
-
-
1
-
-
-
2
16
-
-
-
3
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
14
-
-
-
-
4
-
-
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-
1
-
-
-
-
-
2
4
16
-
-
-
3
-
-
-
-
-
-
-
-
12
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
645161
Liou
Characterization of human UMP/ ...
Homo sapiens
Cancer Res.
62
1624-1631
2002
-
-
1
-
-
-
4
16
1
-
-
3
-
3
-
-
1
-
-
1
-
-
27
-
-
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-
-
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1
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4
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16
1
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3
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1
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1
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27
-
-
-
-
-
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-
-
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-
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645160
Pearman
Characterization of human UMP- ...
Homo sapiens
Life Sci.
69
2361-2370
2001
-
-
-
-
-
-
-
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-
1
1
1
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2
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21
-
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3
1
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1
1
1
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21
-
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3
1
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-
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645152
Hutter
Phosphoryl transfer by a conce ...
Dictyostelium discoideum
Protein Sci.
9
2225-2231
2000
-
-
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-
-
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1
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1
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1
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645156
Van Rompay
Phosphorylation of deoxycytidi ...
Homo sapiens
Mol. Pharmacol.
56
562-569
1999
-
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1
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5
2
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3
-
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9
-
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1
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5
2
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9
-
-
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-
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-
-
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-
-
645153
Zhou
Cloning, expression in Escheri ...
Arabidopsis thaliana
Plant Physiol.
117
245-254
1998
-
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1
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1
4
-
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1
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5
-
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1
1
-
-
-
-
2
1
-
-
1
2
1
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
2
4
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
1
2
1
-
-
-
-
-
-
-
-
-
645157
Okajima
Exchange of nucleoside monopho ...
Sus scrofa
J. Biochem.
124
359-367
1998
-
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1
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2
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3
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3
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2
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3
3
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1
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2
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3
-
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2
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3
3
-
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-
-
-
-
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-
-
645158
Zhou
Site-specific mutations of con ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
358
297-302
1998
-
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7
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2
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2
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7
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Seagrave
Pyrimidine nucleoside monophos ...
Rattus norvegicus
Arch. Biochem. Biophys.
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645147
Seagrave
Pyrimidine nucleoside monophos ...
Rattus norvegicus
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1986
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209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
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1001-1005
1983
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645149
Kohno
Purification and properties of ...
Saccharomyces cerevisiae
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1983
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645141
Scott
Kinetics and equilibria of pyr ...
Homo sapiens
Biochim. Biophys. Acta
571
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1979
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645139
Orengo
Pyrimidine nucleoside monophos ...
Rattus norvegicus
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645140
Anderson
UMP-CMP kinase from Tetrahymen ...
Tetrahymena pyriformis
Methods Enzymol.
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331-337
1978
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Kobayashi
NADPH activation of deoxycytid ...
Rattus norvegicus
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645142
Maness
Activation of rat liver pyrimi ...
Rattus norvegicus
Biochim. Biophys. Acta
429
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1976
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Teng
Human erythrocyte pyrimidine n ...
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645143
Maness
A pyrimidine nucleoside monoph ...
Rattus norvegicus
Biochemistry
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1975
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645148
Gravey
ATP:UMP-CMP phosphotransferase ...
Tetrahymena pyriformis
Biochim. Biophys. Acta
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38-49
1973
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Ruffner
Adenosine triphosphate: uridin ...
Tetrahymena pyriformis
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1969
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Hurwitz
The enzymatic incorporation of ...
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