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Literature summary for 2.7.4.14 extracted from
- Pyrimidine nucleoside monophosphate kinase from rat liver and rat Novikoff ascites hepatoma (EC 2.7.4.14) (1978), Methods Enzymol., 51, 321-331.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CMP | above 0.13 mM, substrate inhibition | Rattus norvegicus | |
| F- | complete inhibition at 25 mM | Rattus norvegicus | |
| additional information | no substrate inhibition with 3 mM dCMP and 1 mM UMP | Rattus norvegicus | |
| NaClO | 250 mM | Rattus norvegicus | |
| NaSCN | 250 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0053 | - |
CMP | reaction with ATP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
| 0.027 | - |
dCMP | reaction with dATP, liver enzyme | Rattus norvegicus | |
| 0.03 | - |
CMP | reaction with ATP, liver enzyme | Rattus norvegicus | |
| 0.04 | - |
UMP | reaction with ATP, liver enzyme | Rattus norvegicus | |
| 0.043 | - |
UMP | reaction with ATP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
| 0.053 | - |
UMP | reaction with dATP, liver enzyme | Rattus norvegicus | |
| 0.074 | - |
dATP | reaction with CMP, liver enzyme | Rattus norvegicus | |
| 0.134 | - |
ATP | reaction with dCMP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
| 0.32 | - |
ATP | reaction with CMP, liver enzyme | Rattus norvegicus | |
| 0.42 | - |
dATP | reaction with UMP, liver enzyme | Rattus norvegicus | |
| 0.58 | - |
ATP | reaction with UMP, liver enzyme | Rattus norvegicus | |
| 0.61 | - |
dATP | reaction with dCMP, liver enzyme | Rattus norvegicus | |
| 0.68 | - |
ATP | reaction with dCMP, liver enzyme | Rattus norvegicus | |
| 0.715 | - |
dCMP | reaction with ATP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
| 0.82 | - |
dCTP | reaction with CMP, liver enzyme | Rattus norvegicus | |
| 0.98 | - |
CMP | reaction with dCTP, liver enzyme | Rattus norvegicus | |
| 1.1 | - |
dCMP | reaction with dATP, liver enzyme | Rattus norvegicus | |
| 2.77 | - |
dCMP | reaction with ATP, liver enzyme | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
| Mg2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
| Mn2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
| Na2SO4 | 250 mM, stimulates | Rattus norvegicus | |
| NaCl | 250 mM, stimulates | Rattus norvegicus | |
| Ni2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| Novikoff ascites hepatoma cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
reaction with CMP and ATP | Rattus norvegicus |
| 4.98 | - |
reaction with dCMP and ATP | Rattus norvegicus |
| 8.76 | - |
reaction with UMP and dCMP | Rattus norvegicus |
| 30 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 25 mM Tris-acetate buffer, pH 7.5, 50 mM 2-mercaptoethanol, 50% glycerol, stable for at least 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + CMP | - |
Rattus norvegicus | ADP + CDP | - |
? | |
| ATP + dCMP | - |
Rattus norvegicus | ADP + dCDP | - |
? | |
| ATP + UMP | - |
Rattus norvegicus | ADP + UDP | - |
? | |
| dATP + CMP | - |
Rattus norvegicus | dADP + CDP | - |
? | |
| dATP + dCMP | - |
Rattus norvegicus | dADP + dCDP | - |
? | |
| dATP + UMP | - |
Rattus norvegicus | dADP + ADP | - |
? | |
| dCTP + CMP | - |
Rattus norvegicus | dCDP + CDP | - |
? | |
| ITP + UMP | - |
Rattus norvegicus | IDP + UDP | - |
? | |
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